公开(公告)号：US5149633A

公开(公告)日：1992-09-22

申请号：US249381

申请日：1988-09-26

申请人： Bernd Vogt ,  Liselotte Schellong ,  Joachim Siedel ,  Joachim Ziegenhorn

发明人： Bernd Vogt ,  Liselotte Schellong ,  Joachim Siedel ,  Joachim Ziegenhorn

IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/30 ,  C12Q1/34 ,  G01N33/66 ,  G01N33/96

CPC分类号： C12Q1/26 ,  C12Q1/34 ,  G01N33/66 ,  G01N33/96 ,  C12Q2326/92 ,  Y10S436/904 ,  Y10T436/144444 ,  Y10T436/173845 ,  Y10T436/25 ,  Y10T436/25125

摘要： Process for the specific determination of the serum fructosamine content in blood or in samples obtained from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, in which before the color reaction sample components with a non-specific reducing action and/or causing turbidity are removed and subsequently the color reagent is added at a pH value of from 10 to 12. The sample components are removed by treatment at approximately neutral pH value with a reagent composition comprising at least one enzymatic oxidation agent, optionally together with peroxidase and/or catalase and/or lipase, as well as with at least one SH group-blocking substance. A kit for the specific determination of the serum fructosamine content in blood or samples obtained from blood, comprises said reagent composition, a rebuffering reagent with a buffer which has an alkaline pH value and a color reagent for the detection of fructosamine.

摘要翻译： 通过与适当的着色剂反应，测定血液中或血液中得到的样品中的血清果糖胺含量的特异性测定方法，由此产生颜色变化，其中在着色反应之前，具有非特异性还原作用的样品成分 和/或引起浊度被除去，随后在10至12的pH值下加入显色试剂。通过用大约中性pH值处理的样品组分除去，试剂组合物包含至少一种酶氧化剂，任选地一起 与过氧化物酶和/或过氧化氢酶和/或脂肪酶，以及至少一种SH基团阻断物质。 用于特异性测定血液或血液中的血清果糖胺含量的试剂盒包括所述试剂组合物，具有碱性pH值的缓冲液的再吸附剂和用于检测果糖胺的显色剂。