Process for the microbial production of alcoholoxidase
    2.
    发明授权
    Process for the microbial production of alcoholoxidase 失效
    藻酸酶微生物生产工艺

    公开(公告)号:US4250261A

    公开(公告)日:1981-02-10

    申请号:US55533

    申请日:1979-07-09

    IPC分类号: C12N9/04 C12R1/78

    CPC分类号: C12N9/0006 Y10S435/93

    摘要: A method of producing alcohol oxidase wherein Hansenula polymorpha yeast is cultivated in a nutrient broth containing inorganic nutrient salts of which either the phosphate, nitrate or potassium is present in a cell-growth-limiting concentration at a temperature of 25.degree. to 45.degree. C. and a pH of 4.0 to 6.0 with oxygen being supplied as air for a mixture of air and oxygen. According to the invention, an organic substrate consisting of glycerin, sorbite or xylose, noncatabolite-repressive to alcohol-oxidase is added to the broth in a concentration of 0.1 to 1.0% in a nutrient solution at a rate of 0.03 to 0.15 .sup.-1.

    摘要翻译: 一种生产醇氧化酶的方法,其中将多形汉逊酵母(Hansenula polymorpha yeast)培养在含有无机营养盐的营养肉汤中,其中磷酸盐,硝酸盐或钾以25至45℃的温度存在于细胞生长限制浓度中。 pH为4.0至6.0,氧气作为空气和氧气的混合物供应。 根据本发明,在营养液中以0.03-0.15-1的比例,在肉汤中以0.1至1.0%的浓度加入由甘油,山梨糖醇或木糖组成的有机底物,对醇醇氧化酶具有抑制作用。

    Method of extracting uranium from sea water
    3.
    发明授权
    Method of extracting uranium from sea water 失效
    从海水中提取铀的方法

    公开(公告)号:US4263403A

    公开(公告)日:1981-04-21

    申请号:US24957

    申请日:1979-03-29

    摘要: A method of producing a matrix of micro-organisms for extracting and gaining uranium from sea water, according to which the matrix is made of micro-organisms occurring in nature that are exposed to x-ray irradiations. These predetermined micro-organisms accordingly form colonies or threads and are filtered easily and cultivated. These micro-organisms accordingly form colonies or threads and are filtered easily and cultivated. These micro-organisms have a ratio of surface to volume that is relatively high. Subsequently, the thus irradiated micro-organisms are placed on a nutrient medium rich in uranium and are left there until colonies are formed. Thereupon, the surviving colonies of mutants are inoculated in a nutrient solution, and are then cultivated to produce the matrix.

    摘要翻译: 一种生产用于从海水中提取和获得铀的微生物基质的方法,根据该方法,基质由暴露于x射线照射的大自然中发生的微生物制成。 这些预定的微生物相应地形成菌落或丝线,并被容易地过滤并培养。 这些微生物相应地形成菌落或丝线,并被容易地过滤并培养。 这些微生物的表面与体积的比例相对较高。 随后,将如此照射的微生物置于富含铀的营养培养基上,并留在此处,直到菌落形成。 因此,将存活的突变体菌落接种在营养液中,然后培养以产生基质。