公开(公告)号：US20110053139A1

公开(公告)日：2011-03-03

申请号：US12069284

申请日：2008-02-08

申请人： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

发明人： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

IPC分类号： C12Q1/70 ,  G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

摘要： Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

公开(公告)号：US20140249042A1

公开(公告)日：2014-09-04

申请号：US14172429

申请日：2014-02-04

申请人： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

发明人： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

IPC分类号： G01N33/543 ,  C12Q1/68

CPC分类号： G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

摘要： Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

公开(公告)号：US20080108057A1

公开(公告)日：2008-05-08

申请号：US11158653

申请日：2005-06-22

申请人： Jeffrey Griffith ,  Christopher Heaphy ,  Marco Bisoffi ,  William Hines

发明人： Jeffrey Griffith ,  Christopher Heaphy ,  Marco Bisoffi ,  William Hines

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/16

摘要： Methods for assessing the extent of allelic imbalance in a genomic nucleic acid sample. Methods for diagnosing cancer and determining the prognosis of a patient with cancer, including breast or prostate cancer, by assessing the extent of allelic imbalance in a genomic nucleic acid sample.

摘要翻译： 评估基因组核酸样品中等位基因不平衡程度的方法。 通过评估基因组核酸样品中等位基因不平衡的程度来诊断癌症的方法和确定癌症患者（包括乳腺癌或前列腺癌）的预后。

公开(公告)号：US08709791B2

公开(公告)日：2014-04-29

申请号：US12069284

申请日：2008-02-08

申请人： Richard S Larson ,  Brian Hjelle ,  Pam R Hall ,  David C Brown ,  Marco Bisoffi ,  Susan M Brozik ,  Darren W Branch ,  Thayne L Edwards ,  David Wheeler

发明人： Richard S Larson ,  Brian Hjelle ,  Pam R Hall ,  David C Brown ,  Marco Bisoffi ,  Susan M Brozik ,  Darren W Branch ,  Thayne L Edwards ,  David Wheeler

IPC分类号： G01N33/543

CPC分类号： G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

摘要： A biosensor combining the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In a preferred embodiment, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV). Rapid detection of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, and the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1 The biosensor was able to delect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS).

摘要翻译： 将在325MHz频率产生的表面声波（SAW）的灵敏度与由抗体和其他配体提供的特异性检测病毒剂的灵敏度组合的生物传感器。 在优选实施例中，使用具有三个测试和一个参考延迟线的具有二氧化硅波导传感器平台的基于钽酸锂的SAW换能器来吸附针对柯萨奇病毒B4或负链A类生物剂Sin Nombre病毒（SNV）的抗体。 病毒颗粒增加浓度的快速检测在两种病毒的数量级范围内是线性的，并且传感器对其靶标的选择性没有被混合的单纯疱疹病毒1型的存在所影响。生物传感器能够以剂量剔除SNV 低于患有汉坦病毒心肺综合征（HCPS）的人类患者中通常发现的病毒的负载。