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    Detection of bioagents using a shear horizontal surface acoustic wave biosensor
    2.
    发明授权
    Detection of bioagents using a shear horizontal surface acoustic wave biosensor 有权
    使用剪切水平表面声波生物传感器检测生物标签

    公开(公告)号：US08709791B2

    公开(公告)日：2014-04-29

    申请号：US12069284

    申请日：2008-02-08

    Applicant: Richard S Larson ,  Brian Hjelle ,  Pam R Hall ,  David C Brown ,  Marco Bisoffi ,  Susan M Brozik ,  Darren W Branch ,  Thayne L Edwards ,  David Wheeler

    Inventor： Richard S Larson ,  Brian Hjelle ,  Pam R Hall ,  David C Brown ,  Marco Bisoffi ,  Susan M Brozik ,  Darren W Branch ,  Thayne L Edwards ,  David Wheeler

    IPC: G01N33/543

    CPC classification number: G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

    Abstract: A biosensor combining the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In a preferred embodiment, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV). Rapid detection of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, and the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1 The biosensor was able to delect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS).

    Abstract translation: 将在325MHz频率产生的表面声波（SAW）的灵敏度与由抗体和其他配体提供的特异性检测病毒剂的灵敏度组合的生物传感器。 在优选实施例中，使用具有三个测试和一个参考延迟线的具有二氧化硅波导传感器平台的基于钽酸锂的SAW换能器来吸附针对柯萨奇病毒B4或负链A类生物剂Sin Nombre病毒（SNV）的抗体。 病毒颗粒增加浓度的快速检测在两种病毒的数量级范围内是线性的，并且传感器对其靶标的选择性没有被混合的单纯疱疹病毒1型的存在所影响。生物传感器能够以剂量剔除SNV 低于患有汉坦病毒心肺综合征（HCPS）的人类患者中通常发现的病毒的负载。

    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR
    3.
    发明申请
    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR 审中-公开

    公开(公告)号：US20140249042A1

    公开(公告)日：2014-09-04

    申请号：US14172429

    申请日：2014-02-04

    Applicant: Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    Inventor： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC: G01N33/543 ,  C12Q1/68

    CPC classification number: G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

    Abstract: Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

    Detection of bioagents using a shear horizontal surface acoustic wave biosensor
    4.
    发明申请
    Detection of bioagents using a shear horizontal surface acoustic wave biosensor 有权

    公开(公告)号：US20110053139A1

    公开(公告)日：2011-03-03

    申请号：US12069284

    申请日：2008-02-08

    Applicant: Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    Inventor： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC: C12Q1/70 ,  G01N33/53 ,  C12M1/34

    CPC classification number: G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

    Abstract: Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

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