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    Glucan lyase producing 1,5-anhydrofructose
    2.
    发明授权
    Glucan lyase producing 1,5-anhydrofructose 失效
    葡聚糖裂解酶产生1,5-脱水果糖

    公开(公告)号：US5695970A

    公开(公告)日：1997-12-09

    申请号：US416709

    申请日：1995-04-18

    申请人： Shukun Yu ,  Marianne Pedersen ,  Lennart Kenne

    发明人： Shukun Yu ,  Marianne Pedersen ,  Lennart Kenne

    IPC分类号： C07H3/10 ,  C12N9/88 ,  C12P19/02 ,  C12N1/20

    CPC分类号： C12P19/02 ,  C07H3/10 ,  C12N9/88

    摘要： The invention relates to an enzyme, exo-.alpha.-1,4-glucan lyase, capable of successively cleaving the terminal .alpha.-1,4-D-glucosidic bonds from the non-reducing ends of an .alpha.-1,4-glucan. The enzyme is selected from the group consisting of .alpha.-1,4-glucan lyase isolated from an alga and functional derivatives and analogs derived from a nucleotide sequence related to an alga .alpha.-1,4-glucan lyase. Further, a method of enzymatically cleaving the terminal .alpha.-1,4-D-glucosidic bonds from the non-reducing ends of an .alpha.-1,4-glucan, and the degradation product 1,5-anhydrofructose for use as an oxygen radical scavenger or anti-oxidant and/or as a sugar substitute, are disclosed. Additionally, a method of producing an enzyme capable of successively cleaving the terminal .alpha.-1,4-glucosidic bonds from the non-reducing ends of an .alpha.-1,4-glucan; an antibody which binds to the amino acid sequence of the enzyme, and a DNA or RNA probe which recognizes a nucleotide sequence coding for the enzyme, are described.

    摘要翻译： PCT No.PCT / SE93 / 00854 Sec。 371日期1995年04月18日 102（e）1995年4月18日PCT PCT 1993年10月19日PCT公布。 出版物WO94 / 09122 日期1994年04月28日本发明涉及能够从α-1的非还原末端连续切割末端α-1,4-D-糖苷键的酶，外-α-1,4-葡聚糖裂解酶 ，4-葡聚糖。 该酶选自由藻类分离的α-1,4-葡聚糖裂解酶和功能性衍生物以及衍生自与藻类α-1,4-葡聚糖裂解酶相关的核苷酸序列的类似物。 此外，从α-1,4-葡聚糖的非还原末端酶切地切割末端α-1,4- D - 糖苷键的方法，以及用作氧自由基的降解产物1,5-脱水果糖 清除剂或抗氧化剂和/或作为糖代用品。 另外，生产能够从α-1,4-葡聚糖的非还原性末端依次切断末端α-1,4-糖苷键的酶的方法; 描述了结合酶的氨基酸序列的抗体和识别编码该酶的核苷酸序列的DNA或RNA探针。