公开(公告)号：US5334531A

公开(公告)日：1994-08-02

申请号：US837659

申请日：1992-02-14

申请人： Marina Del Bue ,  Paola Cosmina ,  Elisabetta Franchi ,  Guido Grandi

发明人： Marina Del Bue ,  Paola Cosmina ,  Elisabetta Franchi ,  Guido Grandi

IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/61 ,  C12N1/21 ,  C12N15/18 ,  C12N15/70 ,  C12N15/75 ,  C12P21/02 ,  C12R1/07 ,  C12R1/125 ,  G01N33/50

CPC分类号： C12N15/70 ,  C07K14/61 ,  C12N15/75 ,  A61K38/00 ,  Y10S435/832 ,  Y10S435/839

摘要： The description relates to a plasmid vector expression in Bacillus and used for cloning the structural gene which codes for the human growth hormone, a recombinant DNA molecule comprising plasmid vector and the structural gene of the human growth hormone and a method of preparing the hormone comprising inserting the recombinant DNA molecule into strains of Bacillus and cultivating the resulting transformed strains in a suitable culture environment and recovering the thus-synthesized hormone from the cells.

摘要翻译： 描述涉及在芽孢杆菌中的质粒载体表达，用于克隆编码人生长激素的结构基因，包含质粒载体和人生长激素的结构基因的重组DNA分子和制备激素的方法，包括插入 将重组DNA分子转化到芽孢杆菌菌株中，并在合适的培养环境中培养所得的转化菌株，并从细胞中回收合成的激素。

公开(公告)号：US5021340A

公开(公告)日：1991-06-04

申请号：US395173

申请日：1989-08-17

申请人： Salvatore Toma ,  Marina Del Bue ,  Antonio Mele ,  Guido Grandi

发明人： Salvatore Toma ,  Marina Del Bue ,  Antonio Mele ,  Guido Grandi

IPC分类号： C12N15/09 ,  C07K14/585 ,  C12N1/20 ,  C12N1/21 ,  C12N9/54 ,  C12N15/00 ,  C12N15/75 ,  C12P21/02 ,  C12R1/125

CPC分类号： C07K14/585 ,  C12N15/75 ,  C12N9/54 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/61 ,  C07K2319/75 ,  Y10S435/839

摘要： A description is given of a cloning vector useful for the expression and secretion of heterologous proteins in Bacillus subtilis, molecules of recombinant DNA formed by the said cloning vector, expediently cut at a restriction site located within the neutral protease gene, and by the sequence of heterologous DNA which codes for the protein of interest, Bacillus subtilis cells transformed with the said molecules and capable of expressing the sequence of heterologous DNA and of producing and secreting the protein coded by the said sequence of heterologous DNA in high yields.The proteins obtained in this manner are useful in the fields of pharmaceuticals and foods, or as compounds for the production of substances which are normally obtained by chemical synthesis.

摘要翻译： 给出了用于在枯草芽孢杆菌中表达和分泌异源蛋白质的克隆载体的描述，由所述克隆载体形成的重组DNA的分子，有利地在位于中性蛋白酶基因内的限制性位点切割，以及序列 编码感兴趣的蛋白质的异源DNA，用所述分子转化的能够表达异源DNA序列的枯草芽孢杆菌细胞，并以高产率产生和分泌由所述异源DNA序列编码的蛋白质。 以这种方式获得的蛋白质可用于药物和食品领域，或用作生产通常通过化学合成获得的物质的化合物。