公开(公告)号：US06897036B2

公开(公告)日：2005-05-24

申请号：US10163750

申请日：2002-06-06

Applicant: Hashem Akhavan-Tafti ,  Renuka de Silva ,  Mark Sandison ,  Richard Handley

Inventor： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Mark Sandison ,  Richard Handley

IPC: C12Q1/28 ,  G01N33/533 ,  C12Q1/00 ,  G01N33/53

CPC classification number: C12Q1/28 ,  G01N33/533 ,  Y10S435/968

Abstract: Methods of producing fluorescence from fluorogenic substrates reactive with a peroxidase enzyme are disclosed. Use of the methods in assays for peroxidase enzymes, peroxidase-labeled analytes are provided. Fluorogenic compounds, compositions and kits for reaction with peroxidase enzymes are described. Two modes of producing fluorescent compounds are described.

Abstract translation: 公开了从与过氧化物酶反应的荧光底物产生荧光的方法。 提供了在过氧化物酶，过氧化物酶标记的分析物的测定中使用该方法。 描述了与过氧化物酶反应的荧光化合物，组合物和试剂盒。 描述了产生荧光化合物的两种模式。

公开(公告)号：US5446197A

公开(公告)日：1995-08-29

申请号：US78993

申请日：1993-06-17

Applicant: Mark Sandison ,  Ron Eva ,  Lawrence E. James

Inventor： Mark Sandison ,  Ron Eva ,  Lawrence E. James

IPC: C07C41/40 ,  C07C43/257 ,  C07C43/275 ,  C07C43/285 ,  C07C43/29 ,  C07C201/16 ,  C07C205/59 ,  C07C205/00

CPC classification number: C07C201/16

Abstract: A method of purifying acifluorfen to achieve a purity of greater than about 85% by weight involves heating to dissolution a solution of an acifluorfen crude wet cake and at least one solvent from the group of 1,2-dichlorobenzene, monochlorobenzene, o-xylene and p-xylene, with the solution having from about 10 to about 30 weight percent of acifluorfen crude wet cake. The-solution is then cooled until crystals of purified acifluorfen form. The crystals are then filtered at a temperature within the range of from about 0.degree. to about 30.degree. C. The filtered crystals are then recovered.

Abstract translation: 净化三氟果霜以达到大于约85重量％的纯度的方法涉及加热溶解氟锁草醚粗湿滤饼和至少一种来自1,2-二氯苯，一氯苯，邻二甲苯和 对二甲苯，溶液具有约10至约30重量％的三氟果霜粗湿饼。 然后将溶液冷却至纯化的三氟果霜形成晶体。 然后将晶体在约0℃至约30℃的温度范围内过滤。然后回收过滤的晶体。

公开(公告)号：US20050106577A1

公开(公告)日：2005-05-19

申请号：US10715284

申请日：2003-11-17

Applicant: Hashem Akhavan-Tafti ,  Renuka Silva ,  Robert Eickholt ,  C. Gundlach ,  Richard Handley ,  Kenneth Lauwers ,  Mark Sandison ,  Wenhua Xie

Inventor： Hashem Akhavan-Tafti ,  Renuka Silva ,  Robert Eickholt ,  C. Gundlach ,  Richard Handley ,  Kenneth Lauwers ,  Mark Sandison ,  Wenhua Xie

IPC: C07H21/04 ,  C12M1/34 ,  C12Q1/68

CPC classification number: C07H21/04

Abstract: Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

Abstract translation: 公开了用于结合核酸的固相材料及其使用方法。 这些材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 固相材料包括固体支持部分，其包含选自二氧化硅，玻璃，不溶性合成聚合物的基质和与核酸结合部分连接以吸附和结合核酸的不溶性多糖，所述核酸结合部分（NAB）为 通过可切割的连接部分连接到固体支持部分。 优选的核酸结合部分包含三元或四元鎓基团。 材料可以是微粒，纤维，珠，膜，试管或微孔的形式，并且还可以包括磁芯部分。 在分离或纯化核酸的方法中公开了使用可切割固体支持物结合核酸的方法。

公开(公告)号：US5847130A

公开(公告)日：1998-12-08

申请号：US905578

申请日：1997-08-04

Applicant: Mark Sandison

Inventor： Mark Sandison

IPC: C07D295/24

CPC classification number: C07D295/24

Abstract: Aqueous solutions of N-methylmorpholine N-oxide having reduced nitrosamine concentrations are produced by adding catalytic amounts of inorganic carbonate or bicarbonate or carboxylic acid or carboxylate salt.

Abstract translation: 通过加入催化量的无机碳酸盐或碳酸氢盐或羧酸或羧酸盐制备具有降低的亚硝胺浓度的N-甲基吗啉N-氧化物的水溶液。

公开(公告)号：US5693796A

公开(公告)日：1997-12-02

申请号：US702415

申请日：1996-08-14

Applicant: Mark Sandison

Inventor： Mark Sandison

IPC: C07D295/24

CPC classification number: C07D295/24

Abstract: Aqueous solutions of N-methylmorpholine N-oxide having reduced nitrosamine concentrations are produced by adding catalytic amounts of inorganic carbonate or bicarbonate or carboxylic acid or carboxylate salt.

Abstract translation: 通过加入催化量的无机碳酸盐或碳酸氢盐或羧酸或羧酸盐制备具有降低的亚硝胺浓度的N-甲基吗啉N-氧化物的水溶液。