公开(公告)号：US07928236B2

公开(公告)日：2011-04-19

申请号：US11916061

申请日：2006-06-08

申请人： Masumi Taki ,  Masahiko Sisido

发明人： Masumi Taki ,  Masahiko Sisido

IPC分类号： C07D221/18 ,  A61K38/02

CPC分类号： C09B15/00 ,  C07K14/005 ,  C09B5/36 ,  C12N2740/16322 ,  Y02P20/55

摘要： The subject of the present invention is to provide a fluorescent substance excitable under visible light, having higher photostability and a long fluorescence lifetime. Another subject is to provide a fluorescent substance consists of a non-natural amino acid applicable to peptide synthesis systems.Searching fluorescent substances, which are fluorescent amino acids and excitable under visible light, having the lowest possible molecular weight for a high photostability resulted in forming a condensed polycyclic aromatic compound by subjecting a compound having an acridone structure to substitution with an amino acid and further condensation with a benzene ring. Thus, the subject is achieved by the fluorescent substance consisting of amino acid-substituted benzoacridone derivative excitable under visible light.

摘要翻译： 本发明的主题是提供在可见光下可激发的荧光物质，具有较高的光稳定性和较长的荧光寿命。 另一个主题是提供由适用于肽合成系统的非天然氨基酸组成的荧光物质。 在具有高光稳定性的情况下，以具有最低可能分子量的荧光氨基酸和可见光激发的荧光物质搜索导致通过使具有吖啶酮结构的化合物用氨基酸取代并进一步缩合形成稠合多环芳族化合物 与苯环。 因此，通过由可见光可激发的氨基酸取代的苯并吖啶酮衍生物构成的荧光物质来实现。

公开(公告)号：US07847098B2

公开(公告)日：2010-12-07

申请号：US12306784

申请日：2007-07-03

申请人： Masumi Taki ,  Masahiko Sisido

发明人： Masumi Taki ,  Masahiko Sisido

IPC分类号： C07D213/00 ,  A61K38/00

CPC分类号： C07D219/06 ,  C09K11/06 ,  C09K2211/1029

摘要： The present invention provides a fluorescent amino acid derivative which can be synthesized by simple steps, can be excited particularly by a blue laser ray region of visible light, and has an improved light stability. These objects can be achieved by a fluorescent amino acid derivative which is an acridone derivative substituted with an amino acid to comprise an electrophilic substituent group between the amino acid and the acridone derivative. Instead of a conventional strategy that aminophenylalanine is used as a starting material to form a fluorescent group through a coupling reaction and an intramolecular cyclization reaction, a fluorescent acridone derivative is used as a starting material to furnish the material to a reactive group by a position-specific electrophilic substitution reaction, and then the acridone derivative having the reactive group is allowed to couple with an amino acid derivative.

摘要翻译： 本发明提供了可以通过简单的步骤合成的荧光氨基酸衍生物，特别可以通过可见光的蓝色激光射线区域被激发，并且具有改善的光稳定性。 这些目的可以通过荧光氨基酸衍生物来实现，其是由氨基酸取代的吖啶酮衍生物，其在氨基酸和吖啶酮衍生物之间包含亲电取代基。 代替使用氨基苯丙氨酸作为通过偶联反应和分子内环化反应形成荧光基团的起始材料的常规策略，使用荧光吖啶酮衍生物作为原料，通过位置 - 取代基将该材料提供给反应性基团， 特异性亲电取代反应，然后使具有反应性基团的吖啶酮衍生物与氨基酸衍生物偶联。

公开(公告)号：US20100152417A1

公开(公告)日：2010-06-17

申请号：US11916061

申请日：2006-06-08

申请人： Masumi Taki ,  Masahiko Sisido

发明人： Masumi Taki ,  Masahiko Sisido

IPC分类号： C07K2/00 ,  C07D221/18 ,  C07K14/00

CPC分类号： C09B15/00 ,  C07K14/005 ,  C09B5/36 ,  C12N2740/16322 ,  Y02P20/55

摘要： The subject of the present invention is to provide a fluorescent substance excitable under visible light, having higher photostability and a long fluorescence lifetime. Another subject is to provide a fluorescent substance consists of a non-natural amino acid applicable to peptide synthesis systems.Searching fluorescent substances, which are fluorescent amino acids and excitable under visible light, having the lowest possible molecular weight for a high photostability resulted in forming a condensed polycyclic aromatic compound by subjecting a compound having an acridone structure to substitution with an amino acid and further condensation with a benzene ring. Thus, the subject is achieved by the fluorescent substance consisting of amino acid-substituted benzoacridone derivative excitable under visible light.

摘要翻译： 本发明的主题是提供在可见光下可激发的荧光物质，具有较高的光稳定性和较长的荧光寿命。 另一个主题是提供由适用于肽合成系统的非天然氨基酸组成的荧光物质。 在具有高光稳定性的情况下，以具有最低可能分子量的荧光氨基酸和可见光激发的荧光物质搜索导致通过使具有吖啶酮结构的化合物用氨基酸取代并进一步缩合形成稠合多环芳族化合物 与苯环。 因此，通过由可见光可激发的氨基酸取代的苯并吖啶酮衍生物构成的荧光物质来实现。

公开(公告)号：US20080220477A1

公开(公告)日：2008-09-11

申请号：US12107435

申请日：2008-04-22

申请人： Takahiro Hohsaka ,  Masahiko Sisido

发明人： Takahiro Hohsaka ,  Masahiko Sisido

IPC分类号： C07K16/40 ,  C12N15/13 ,  C07C229/52 ,  C12P21/06 ,  C07K14/435

CPC分类号： C07B59/001 ,  C07F5/022 ,  C07F5/027 ,  C09B57/00 ,  C09B57/10 ,  C12P21/02

摘要： This invention relates to: a labeled amino acid that can be introduced into a protein with the aid of a protein synthesis system; a functional protein having functions derived from a label compound; a labeled amino acid comprising an aromatic ring bound to an amino acid side chain and a label compound bound thereto via the aromatic ring; a functional protein to which the labeled amino acid has been introduced; and a novel method for effectively obtaining a labeled amino acid-tRNA complex.

摘要翻译： 本发明涉及：可以借助蛋白质合成系统引入蛋白质的标记氨基酸; 具有衍生自标记化合物的功能的功能性蛋白质; 包含与氨基酸侧链结合的芳环和经由芳环结合的标记化合物的标记氨基酸; 已经引入了标记的氨基酸的功能性蛋白质; 以及有效获得标记的氨基酸-TRNA复合物的新方法。

公开(公告)号：US20080096259A1

公开(公告)日：2008-04-24

申请号：US11907430

申请日：2007-10-12

申请人： Masahiko Sisido ,  Keiko Ninomiya

发明人： Masahiko Sisido ,  Keiko Ninomiya

IPC分类号： C12P19/34

CPC分类号： C07H21/02

摘要： It is intended to provide a method of chemically synthesizing an aminoacyl tRNA which is completely different from the existing methods, namely, a highly efficient and practically usable method for synthesizing an aminoacyl tRNA whereby any nonnatural amino acid can be conveniently aminoacylated without a need for genetic engineering techniques and detected without resort to a radioactive isotope. The above-described aminoacylation method comprises enclosing a tRNA and an amino acid in the vicinity of the micelle-water interface and bringing them close to each other to thereby react the same, or providing between them a peptide nucleic acid specifically binding to the tRNA as an antisense molecule and bringing them close to each other to thereby react the same.

摘要翻译： 旨在提供一种化学合成氨基tRNA的方法，其与现有方法完全不同，即用于合成氨酰tRNA的高效和实际可用的方法，由此任何非天然氨基酸可以方便地氨基酰化而不需要遗传 检测到工程技术无法获得放射性同位素。 上述氨基酰化方法包括在胶束 - 水界面附近包围tRNA和氨基酸并使它们彼此接近以使其反应，或在它们之间提供与tRNA特异性结合的肽核酸作为 反义分子并使它们彼此接近，从而使其反应。

公开(公告)号：US20060020111A1

公开(公告)日：2006-01-26

申请号：US10527156

申请日：2003-09-05

申请人： Masahiko Sisido ,  Keiko Ninomiya

发明人： Masahiko Sisido ,  Keiko Ninomiya

IPC分类号： C07H21/02 ,  C07K1/02

CPC分类号： C07H21/02

摘要： It is intended to provide a method of chemically synthesizing an aminoacyl tRNA which is completely different from the existing methods, namely, a highly efficient and practically usable method for synthesizing an aminoacyl tRNA whereby any nonnatural amino acid can be conveniently aminoacylated without a need for genetic engineering techniques and detected without resort to a radioactive isotope. The above-described aminoacylation method comprises enclosing a tRNA and an amino acid in the vicinity of the micelle-water interface and bringing them close to each other to thereby react the same, or providing between them a peptide nucleic acid specifically binding to the tRNA as an antisense molecule and bringing them close to each other to thereby react the same.

摘要翻译： 旨在提供一种化学合成氨基tRNA的方法，其与现有方法完全不同，即用于合成氨酰tRNA的高效和实际可用的方法，由此任何非天然氨基酸可以方便地氨基酰化而不需要遗传 检测到工程技术无法获得放射性同位素。 上述氨基酰化方法包括在胶束 - 水界面附近包围tRNA和氨基酸并使它们彼此接近以使其反应，或在它们之间提供与tRNA特异性结合的肽核酸作为 反义分子并使它们彼此接近，从而使其反应。

公开(公告)号：US20090240026A1

公开(公告)日：2009-09-24

申请号：US12306784

申请日：2007-07-03

申请人： Masumi Taki ,  Masahiko Sisido

发明人： Masumi Taki ,  Masahiko Sisido

IPC分类号： C07K2/00 ,  C07D219/00

CPC分类号： C07D219/06 ,  C09K11/06 ,  C09K2211/1029

摘要： An object of the present invention is to provide a novel fluorescent amino acid derivative which can be synthesized by simple steps, can be excited particularly by a blue laser ray region of visible light, and has an improved light stability. Another object is to provide a production method of the fluorescent amino acid derivative. These objects can be achieved by a fluorescent amino acid derivative which is an acridone derivative substituted with an amino acid to comprise an electrophilic substituent group between the amino acid and the acridone derivative. Instead of a conventional strategy that aminophenylalanine is used as a starting material to form a fluorescent group through a coupling reaction and an intramolecular cyclization reaction, a fluorescent acridone derivative is used as a starting material to furnish the material to a reactive group by a position-specific electrophilic substitution reaction, and then the acridone derivative having the reactive group is allowed to couple with an amino acid derivative.

摘要翻译： 本发明的目的是提供可以通过简单的步骤合成的新的荧光氨基酸衍生物，特别可以通过可见光的蓝色激光射线区域被激发，并且具有改善的光稳定性。 另一个目的是提供荧光氨基酸衍生物的制备方法。 这些目的可以通过荧光氨基酸衍生物来实现，其是由氨基酸取代的吖啶酮衍生物，其在氨基酸和吖啶酮衍生物之间包含亲电取代基。 代替使用氨基苯丙氨酸作为通过偶联反应和分子内环化反应形成荧光基团的起始材料的常规策略，使用荧光吖啶酮衍生物作为原料，通过位置 - 取代基将该材料提供给反应性基团， 特异性亲电取代反应，然后使具有反应性基团的吖啶酮衍生物与氨基酸衍生物偶联。

公开(公告)号：US07385038B2

公开(公告)日：2008-06-10

申请号：US10490508

申请日：2003-07-15

申请人： Takahiro Hohsaka ,  Masahiko Sisido

发明人： Takahiro Hohsaka ,  Masahiko Sisido

IPC分类号： C07K16/00

CPC分类号： C07B59/001 ,  C07F5/022 ,  C07F5/027 ,  C09B57/00 ,  C09B57/10 ,  C12P21/02

摘要： This invention relates to: a labeled amino acid that can be introduced into a protein with the aid of a protein synthesis system; a functional protein having functions derived from a label compound; a labeled amino acid comprising an aromatic ring bound to an amino acid side chain and a label compound bound thereto via the aromatic ring; a functional protein to which the labeled amino acid has been introduced; and a novel method for effectively obtaining a labeled amino acid-tRNA complex.

摘要翻译： 本发明涉及：可以借助蛋白质合成系统引入蛋白质的标记氨基酸; 具有衍生自标记化合物的功能的功能性蛋白质; 包含与氨基酸侧链结合的芳环和经由芳环结合的标记化合物的标记氨基酸; 已经引入了标记的氨基酸的功能性蛋白质; 以及有效获得标记的氨基酸-TRNA复合物的新方法。

公开(公告)号：US20070117181A1

公开(公告)日：2007-05-24

申请号：US10490508

申请日：2003-07-15

申请人： Takahiro Hohsaka ,  Masahiko Sisido

发明人： Takahiro Hohsaka ,  Masahiko Sisido

IPC分类号： C07K16/40 ,  C07F5/02 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

CPC分类号： C07B59/001 ,  C07F5/022 ,  C07F5/027 ,  C09B57/00 ,  C09B57/10 ,  C12P21/02

摘要： This invention relates to: a labeled amino acid that can be introduced into a protein with the aid of a protein synthesis system; a functional protein having functions derived from a label compound; a labeled amino acid comprising an aromatic ring bound to an amino acid side chain and a label compound bound thereto via the aromatic ring; a functional protein to which the labeled amino acid has been introduced; and a novel method for effectively obtaining a labeled amino acid-tRNA complex.

摘要翻译： 本发明涉及：可以借助蛋白质合成系统引入蛋白质的标记氨基酸; 具有衍生自标记化合物的功能的功能性蛋白质; 包含与氨基酸侧链结合的芳环和经由芳环结合的标记化合物的标记氨基酸; 已经引入了标记的氨基酸的功能性蛋白质; 以及有效获得标记的氨基酸-TRNA复合物的新方法。