公开(公告)号：US20110020938A1

公开(公告)日：2011-01-27

申请号：US12811573

申请日：2008-11-26

申请人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

发明人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

IPC分类号： C12N15/75 ,  C12N15/63 ,  C12N15/70 ,  C12N1/21 ,  C12N9/88

CPC分类号： C12N9/88 ,  C12Y401/01002

摘要： An object is to provide a means of highly producing an oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains an α-amylase promoter belonging to the genus Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed with this vector to prepare an oxalate decarboxylase producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium and then recovering the oxalate decarboxylase thus produced.

摘要翻译： 目的是提供一种高产生源于微生物的草酸脱羧酶的方法。 构建了包含属于芽孢杆菌属的α-淀粉酶启动子和源自在启动子控制下提供的微生物的草酸脱羧酶基因的重组表达质粒载体。 用该载体转化宿主细菌以制备产生草酸脱羧酶的细菌。 通过培养产生细菌然后回收由此产生的草酸脱羧酶产生重组草酸脱羧酶。

公开(公告)号：US20120135468A1

公开(公告)日：2012-05-31

申请号：US13375860

申请日：2010-04-23

申请人： Toru Katase ,  Yukiko Hoshi ,  Miho Nagaya ,  Shotaro Yamaguchi ,  Masashi Minoda ,  Kazuhiro Nakanishi

发明人： Toru Katase ,  Yukiko Hoshi ,  Miho Nagaya ,  Shotaro Yamaguchi ,  Masashi Minoda ,  Kazuhiro Nakanishi

IPC分类号： C12N9/38 ,  C12N15/63 ,  C12N1/21 ,  A23C9/12 ,  C12N1/15 ,  C12P19/00 ,  C07H3/06 ,  C12N15/56 ,  C12N1/19

CPC分类号： A23C9/1206 ,  A23L29/06 ,  A61K38/47 ,  C12N9/2471 ,  C12Y302/01023

摘要： Disclosed is a novel β-galactosidase. Specifically disclosed are a β-galactosidase derived from Bacillus circulans and a gene for the β-galactosidase. The β-galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.

摘要翻译： 公开了一种新型的半乳糖苷酶。 具体公开的是衍生自环状芽孢杆菌的β-半乳糖苷酶和用于β-半乳糖苷酶的基因。 β-半乳糖苷酶可用于例如生产牛奶，乳制品，发酵乳制品，低聚半乳糖或食品补充剂。

公开(公告)号：US09516888B2

公开(公告)日：2016-12-13

申请号：US13375860

申请日：2010-04-23

申请人： Toru Katase ,  Yukiko Hoshi ,  Miho Nagaya ,  Shotaro Yamaguchi ,  Masashi Minoda ,  Kazuhiro Nakanishi

发明人： Toru Katase ,  Yukiko Hoshi ,  Miho Nagaya ,  Shotaro Yamaguchi ,  Masashi Minoda ,  Kazuhiro Nakanishi

IPC分类号： C12P21/02 ,  A23C9/12 ,  A23L1/03 ,  A61K38/47 ,  C12N9/38

CPC分类号： A23C9/1206 ,  A23L29/06 ,  A61K38/47 ,  C12N9/2471 ,  C12Y302/01023

摘要： Disclosed is a novel β-galactosidase. Specifically disclosed are a β-galactosidase derived from Bacillus circulans and a gene for the β-galactosidase. The β-galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.

摘要翻译： 公开了一种新型β-半乳糖苷酶。 具体公开了衍生自环状芽孢杆菌的β-半乳糖苷酶和β-半乳糖苷酶的基因。 β-半乳糖苷酶可用于例如生产牛奶，乳制品，发酵乳制品，低聚半乳糖或食品补充剂。

公开(公告)号：US08518669B2

公开(公告)日：2013-08-27

申请号：US12811573

申请日：2008-11-26

申请人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

发明人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

IPC分类号： C12P21/02 ,  C12N15/00 ,  C12N9/88

CPC分类号： C12N9/88 ,  C12Y401/01002

摘要： An object is to provide a means of highly producing an oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains an α-amylase promoter belonging to the genus Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed with this vector to prepare an oxalate decarboxylase producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium and then recovering the oxalate decarboxylase thus produced.

摘要翻译： 目的是提供一种高产生源于微生物的草酸脱羧酶的方法。 构建了包含属于芽孢杆菌属的α-淀粉酶启动子和源自在启动子控制下提供的微生物的草酸脱羧酶基因的重组表达质粒载体。 用该载体转化宿主细菌以制备产生草酸脱羧酶的细菌。 通过培养产生细菌然后回收由此产生的草酸脱羧酶产生重组草酸脱羧酶。