公开(公告)号：US20110020938A1

公开(公告)日：2011-01-27

申请号：US12811573

申请日：2008-11-26

申请人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

发明人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

IPC分类号： C12N15/75 ,  C12N15/63 ,  C12N15/70 ,  C12N1/21 ,  C12N9/88

CPC分类号： C12N9/88 ,  C12Y401/01002

摘要： An object is to provide a means of highly producing an oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains an α-amylase promoter belonging to the genus Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed with this vector to prepare an oxalate decarboxylase producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium and then recovering the oxalate decarboxylase thus produced.

摘要翻译： 目的是提供一种高产生源于微生物的草酸脱羧酶的方法。 构建了包含属于芽孢杆菌属的α-淀粉酶启动子和源自在启动子控制下提供的微生物的草酸脱羧酶基因的重组表达质粒载体。 用该载体转化宿主细菌以制备产生草酸脱羧酶的细菌。 通过培养产生细菌然后回收由此产生的草酸脱羧酶产生重组草酸脱羧酶。

公开(公告)号：US08518669B2

公开(公告)日：2013-08-27

申请号：US12811573

申请日：2008-11-26

申请人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

发明人： Takahumi Koyama ,  Yuzo Kojima ,  Kenji Kojima ,  Masashi Minoda

IPC分类号： C12P21/02 ,  C12N15/00 ,  C12N9/88

CPC分类号： C12N9/88 ,  C12Y401/01002

摘要： An object is to provide a means of highly producing an oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains an α-amylase promoter belonging to the genus Bacillus and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the promoter, is constructed. A host bacterium is transformed with this vector to prepare an oxalate decarboxylase producing bacterium. A recombinant oxalate decarboxylase is produced by culturing the producing bacterium and then recovering the oxalate decarboxylase thus produced.

摘要翻译： 目的是提供一种高产生源于微生物的草酸脱羧酶的方法。 构建了包含属于芽孢杆菌属的α-淀粉酶启动子和源自在启动子控制下提供的微生物的草酸脱羧酶基因的重组表达质粒载体。 用该载体转化宿主细菌以制备产生草酸脱羧酶的细菌。 通过培养产生细菌然后回收由此产生的草酸脱羧酶产生重组草酸脱羧酶。

公开(公告)号：US06699496B1

公开(公告)日：2004-03-02

申请号：US09453487

申请日：1999-12-03

申请人： Yuzo Kojima ,  Hiroyasu Andoh

发明人： Yuzo Kojima ,  Hiroyasu Andoh

IPC分类号： A61K4700

CPC分类号： A61K38/465 ,  A23L29/06 ,  A23L33/20 ,  A61K9/2018 ,  A61K9/2054 ,  A61K36/064 ,  C12Y301/01003

摘要： An enzyme in a dosage form for oral use in mammals is provided, wherein the enzyme has an activity of converting fat in a food material into 1,3-diglyceride and/or 1-monoglyceride. The enzyme has never been used conventionally for oral dosing to mammals. The enzyme in an oral dosage form can effectively decrease the body fat and/or internal fat in mammals in the course of general dietary life.

摘要翻译： 提供了用于哺乳动物口服使用的剂型的酶，其中该酶具有将食物中的脂肪转化为1,3-二甘油酯和/或1-单甘油酯的活性。 该酶从未用于哺乳动物的口服给药。 口服剂型中的酶可以在一般膳食生活过程中有效降低哺乳动物体内脂肪和/或内部脂肪。