公开(公告)号：US08880353B2

公开(公告)日：2014-11-04

申请号：US12988037

申请日：2009-04-17

申请人： Hiroshi Nakayama ,  Misaki Akiyama ,  Toshiaki Isobe ,  Masato Taoka ,  Yoshio Yamauchi ,  Nobuhiro Takahashi ,  Hideaki Ishikawa

发明人： Hiroshi Nakayama ,  Misaki Akiyama ,  Toshiaki Isobe ,  Masato Taoka ,  Yoshio Yamauchi ,  Nobuhiro Takahashi ,  Hideaki Ishikawa

IPC分类号： G01N33/48 ,  C12Q1/68 ,  H01J49/00

CPC分类号： C12Q1/6872 ,  H01J49/00 ,  C12Q2521/307

摘要： Disclosed are an apparatus for the identification of a ribonucleic acid, whereby not only the molecular weights of digestion products, from which nothing but the nucleic acid residue composition can be understood, but also nucleic acid residue sequence data is obtained from a product ion mass set and thus the identification reliability for the individual digestion products can be remarkably improved; a method for the identification of a ribonucleic acid; and a program and system for the identification of a ribonucleic acid. The method as described above comprises: searching for nucleic acid sequence database by using a fragment mass set indicating the molecular weights and inner structural data of individual oligonucleotides that are obtained by tandem mass spectrometry on digested oligonucleotides formed by chemically or enzymatically cleaving a ribonucleic acid; scoring candidate sequences; among candidate sequences showing the highest scores, referring those showing scores exceeding the threshold as identified sequences; and using these identified sequences in mapping on nucleic acid sequence database including genomic sequences.

摘要翻译： 公开了用于鉴定核糖核酸的装置，其不仅可以理解除了核酸残基组成之外的消化产物的分子量，还可以从核酸残基序列数据获得产物离子质量组 从而可以显着提高个体消化产品的鉴定可靠性; 用于鉴定核糖核酸的方法; 以及用于鉴定核糖核酸的程序和系统。 如上所述的方法包括：通过使用表示通过串联质谱法获得的各种寡核苷酸的分子量和内部结构数据的片段质谱组来搜索核酸序列数据库对通过化学或酶切割核糖核酸形成的消化的寡核苷酸; 得分候选序列; 在显示最高分数的候选序列中，将显示分数超过阈值的那些指定为鉴定序列; 并使用这些鉴定的序列在包括基因组序列的核酸序列数据库上进行测绘。

公开(公告)号：US20110161273A1

公开(公告)日：2011-06-30

申请号：US12988037

申请日：2009-04-17

申请人： Hiroshi Nakayama ,  Misaki Akiyama ,  Toshiaki Isobe ,  Masato Taoka ,  Yoshio Yamauchi ,  Nobuhiro Takahashi ,  Hideaki Ishikawa

发明人： Hiroshi Nakayama ,  Misaki Akiyama ,  Toshiaki Isobe ,  Masato Taoka ,  Yoshio Yamauchi ,  Nobuhiro Takahashi ,  Hideaki Ishikawa

IPC分类号： G06N5/02

CPC分类号： C12Q1/6872 ,  H01J49/00 ,  C12Q2521/307

摘要： Disclosed are an apparatus for the identification of a ribonucleic acid, whereby not only the molecular weights of digestion products, from which nothing but the nucleic acid residue composition can be understood, but also nucleic acid residue sequence data is obtained from a product ion mass set and thus the identification reliability for the individual digestion products can be remarkably improved; a method for the identification of a ribonucleic acid; and a program and system for the identification of a ribonucleic acid. The method as described above comprises: searching for nucleic acid sequence database by using a fragment mass set indicating the molecular weights and inner structural data of individual oligonucleotides that are obtained by tandem mass spectrometry on digested oligonucleotides formed by chemically or enzymatically cleaving a ribonucleic acid; scoring candidate sequences; among candidate sequences showing the highest scores, referring those showing scores exceeding the threshold as identified sequences; and using these identified sequences in mapping on nucleic acid sequence database including genomic sequences.

摘要翻译： 公开了用于鉴定核糖核酸的装置，其不仅可以理解除了核酸残基组成之外的消化产物的分子量，还可以从核酸残基序列数据获得产物离子质量组 从而可以显着提高个体消化产品的鉴定可靠性; 用于鉴定核糖核酸的方法; 以及用于鉴定核糖核酸的程序和系统。 如上所述的方法包括：通过使用表示通过串联质谱法获得的各种寡核苷酸的分子量和内部结构数据的片段质谱组来搜索核酸序列数据库对通过化学或酶切割核糖核酸形成的消化的寡核苷酸; 得分候选序列; 在显示最高分数的候选序列中，将显示分数超过阈值的那些指定为鉴定序列; 并使用这些鉴定的序列在包括基因组序列的核酸序列数据库上进行测绘。