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1.发明授权公开(公告)号：US08880353B2
公开(公告)日：2014-11-04
申请号：US12988037
申请日：2009-04-17
申请人： Hiroshi Nakayama , Misaki Akiyama , Toshiaki Isobe , Masato Taoka , Yoshio Yamauchi , Nobuhiro Takahashi , Hideaki Ishikawa
发明人： Hiroshi Nakayama , Misaki Akiyama , Toshiaki Isobe , Masato Taoka , Yoshio Yamauchi , Nobuhiro Takahashi , Hideaki Ishikawa
CPC分类号： C12Q1/6872 , H01J49/00 , C12Q2521/307
摘要： Disclosed are an apparatus for the identification of a ribonucleic acid, whereby not only the molecular weights of digestion products, from which nothing but the nucleic acid residue composition can be understood, but also nucleic acid residue sequence data is obtained from a product ion mass set and thus the identification reliability for the individual digestion products can be remarkably improved; a method for the identification of a ribonucleic acid; and a program and system for the identification of a ribonucleic acid. The method as described above comprises: searching for nucleic acid sequence database by using a fragment mass set indicating the molecular weights and inner structural data of individual oligonucleotides that are obtained by tandem mass spectrometry on digested oligonucleotides formed by chemically or enzymatically cleaving a ribonucleic acid; scoring candidate sequences; among candidate sequences showing the highest scores, referring those showing scores exceeding the threshold as identified sequences; and using these identified sequences in mapping on nucleic acid sequence database including genomic sequences.
摘要翻译： 公开了用于鉴定核糖核酸的装置，其不仅可以理解除了核酸残基组成之外的消化产物的分子量，还可以从核酸残基序列数据获得产物离子质量组 从而可以显着提高个体消化产品的鉴定可靠性; 用于鉴定核糖核酸的方法; 以及用于鉴定核糖核酸的程序和系统。 如上所述的方法包括：通过使用表示通过串联质谱法获得的各种寡核苷酸的分子量和内部结构数据的片段质谱组来搜索核酸序列数据库对通过化学或酶切割核糖核酸形成的消化的寡核苷酸; 得分候选序列; 在显示最高分数的候选序列中，将显示分数超过阈值的那些指定为鉴定序列; 并使用这些鉴定的序列在包括基因组序列的核酸序列数据库上进行测绘。
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2.发明申请公开(公告)号：US20110161273A1
公开(公告)日：2011-06-30
申请号：US12988037
申请日：2009-04-17
申请人： Hiroshi Nakayama , Misaki Akiyama , Toshiaki Isobe , Masato Taoka , Yoshio Yamauchi , Nobuhiro Takahashi , Hideaki Ishikawa
发明人： Hiroshi Nakayama , Misaki Akiyama , Toshiaki Isobe , Masato Taoka , Yoshio Yamauchi , Nobuhiro Takahashi , Hideaki Ishikawa
IPC分类号： G06N5/02
CPC分类号： C12Q1/6872 , H01J49/00 , C12Q2521/307
摘要： Disclosed are an apparatus for the identification of a ribonucleic acid, whereby not only the molecular weights of digestion products, from which nothing but the nucleic acid residue composition can be understood, but also nucleic acid residue sequence data is obtained from a product ion mass set and thus the identification reliability for the individual digestion products can be remarkably improved; a method for the identification of a ribonucleic acid; and a program and system for the identification of a ribonucleic acid. The method as described above comprises: searching for nucleic acid sequence database by using a fragment mass set indicating the molecular weights and inner structural data of individual oligonucleotides that are obtained by tandem mass spectrometry on digested oligonucleotides formed by chemically or enzymatically cleaving a ribonucleic acid; scoring candidate sequences; among candidate sequences showing the highest scores, referring those showing scores exceeding the threshold as identified sequences; and using these identified sequences in mapping on nucleic acid sequence database including genomic sequences.
摘要翻译： 公开了用于鉴定核糖核酸的装置，其不仅可以理解除了核酸残基组成之外的消化产物的分子量，还可以从核酸残基序列数据获得产物离子质量组 从而可以显着提高个体消化产品的鉴定可靠性; 用于鉴定核糖核酸的方法; 以及用于鉴定核糖核酸的程序和系统。 如上所述的方法包括：通过使用表示通过串联质谱法获得的各种寡核苷酸的分子量和内部结构数据的片段质谱组来搜索核酸序列数据库对通过化学或酶切割核糖核酸形成的消化的寡核苷酸; 得分候选序列; 在显示最高分数的候选序列中，将显示分数超过阈值的那些指定为鉴定序列; 并使用这些鉴定的序列在包括基因组序列的核酸序列数据库上进行测绘。
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公开(公告)号：US20110089319A1
公开(公告)日：2011-04-21
申请号：US12999434
申请日：2010-06-02
申请人： Toshiaki Isobe , Yoshio Yamauchi
发明人： Toshiaki Isobe , Yoshio Yamauchi
CPC分类号： H01J49/10 , G01N30/7266
摘要： Nano-electrospray ionization techniques include the introduction of a separation solvent containing a sample to a column-integrated needle having a column filled with a resin for liquid chromatography. The separated sample components are sprayed from the tip of the column-integrated needle toward a sample introduction orifice of a mass spectrometer. An organic solvent is simultaneously introduced to a solvent-supplying needle. The organic solvent is supplied from the tip of the solvent-supplying needle to the tip of the column-integrated needle.
摘要翻译： 纳米电喷雾电离技术包括将含有样品的分离溶剂引入具有填充有用于液相色谱的树脂的柱的柱一体化针。 将分离的样品组分从柱集成针的尖端喷射到质谱仪的样品引入孔。 将有机溶剂同时引入溶剂供应针。 有机溶剂从溶剂供应针的尖端供应到列一体化针的末端。
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公开(公告)号：US20050016263A1
公开(公告)日：2005-01-27
申请号：US10479412
申请日：2001-04-08
申请人： Yoshio Yamauchi , Tohru Natsume , Toshiaki Isobe
发明人： Yoshio Yamauchi , Tohru Natsume , Toshiaki Isobe
CPC分类号： G01N30/34 , G01N30/7233 , G01N2030/8411
摘要： The present invention aims to provide a liquid chromatograph, an analysis system, and a gradient elution method, which are designed to permit a gradient change of eluent with higher precision and also permit a high sensitive detection or analysis. For attaining the object, there are provided: a liquid chromatograph having at least a liquid-feeding means 1 for flowing an eluent E at a predetermined flow rate, a sample-pouring means 9 for pouring a sample Sm into the eluent E, and a separation-column means 13 for separating a solute So contained in the sample Sm into components, comprising an eluent-selecting means 8 for forming branched flow paths 7 having predetermined volumes by dividing a flow path 3 of the eluent E fed from the liquid-feeding means 1, temporally storing eluents E1, E2, and soon in the branched flow paths 7, and pouring the sample Sm into the selected eluents E1, E2, and so on, and means for carrying out a gradient elution of a solute So; an analysis system comprising a mass spectrometer connected to the liquid chromatograph, and a gradient-elution method using the liquid chromatograph.
摘要翻译： 本发明旨在提供一种液相色谱仪，分析系统和梯度洗脱方法，其被设计为允许更高精度的洗脱液梯度改变，并且还允许高灵敏度的检测或分析。 为了达到上述目的，提供了一种液相色谱仪，其至少具有用于以预定流量流动洗脱液E的液体供给装置1，用于将样品Sm倾倒入洗脱液E的样品注入装置9，以及 用于将包含在样品Sm中的溶质So分离成分量的分离柱装置13，包括用于形成具有预定体积的分支流路7的洗脱液选择装置8，其中通过将从进液 方法1，在分支流路7中临时储存洗脱液E1，E2并且快速地将样品Sm注入到选定的洗脱液E1，E2等中，以及用于进行溶质So的梯度洗脱的装置; 包括与液相色谱仪连接的质谱仪的分析系统和使用液相色谱仪的梯度洗脱方法。
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公开(公告)号：US06979817B2
公开(公告)日：2005-12-27
申请号：US10450608
申请日：2002-04-08
申请人： Yoshio Yamauchi , Tohru Natsume , Toshiaki Isobe
发明人： Yoshio Yamauchi , Tohru Natsume , Toshiaki Isobe
CPC分类号： H01J49/0013 , H01J49/167
摘要： A problem is to provide a microspray column capable of increasing the ionization efficiency, and a high sensitive mass spectrometer and a mass spectrometric method using such a microspray column. For solving this problem, we provide a column for introducing a sample in an ionization source of a mass spectrometer (ESI/MS) being designed to ionize a sample molecule with electrospray and introduce it into an analyzer, has structural features of: (1) 0.5 μm or less in an inner diameter of a tip opening of the column; (2) 0.5 μm or more and 5 μm or less in a particle size of a column filler; and (3) fritless, a microspray column having the above (1) to (3), a mass spectrometer having such a microspray column in an ionization source, and a mass spectrometric method capable of performing a nonoflow electrospray with the microspray column.
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公开(公告)号：US20050121608A1
公开(公告)日：2005-06-09
申请号：US10450608
申请日：2002-04-08
申请人： Yoshio Yamauchi , Tohru Natsume , Toshiaki Isobe
发明人： Yoshio Yamauchi , Tohru Natsume , Toshiaki Isobe
CPC分类号： H01J49/0013 , H01J49/167
摘要： A problem is to provide a microspray column capable of increasing the ionization efficiency, and a high sensitive mass spectrometer and a mass spectrometric method using such a microspray column. For solving this problem, we provide a column for introducing a sample in an ionization source of a mass spectrometer (ESI/MS) being designed to ionize a sample molecule with electrospray and introduce it into an analyzer, has structural features of: (1) 0.5 μm or less in an inner diameter of a tip opening of the column; (2) 0.5 μm or more and 5 μm or less in a particle size of a column filler; and (3) fritless, a microspray column having the above (1) to (3), a mass spectrometer having such a microspray column in an ionization source, and a mass spectrometric method capable of performing a nonoflow electrospray with the microspray column.
摘要翻译： 问题在于提供能够提高电离效率的微阵列，以及使用这种微球柱的高灵敏度质谱仪和质谱法。 为了解决这个问题，我们提供了一个用于在质谱仪（ESI / MS）的电离源中引入样品的色谱柱，该样品被设计为用电喷雾电离样品分子并将其引入分析仪，具有以下结构特征：（1） 柱的顶端开口的内径为0.5μm或更小; （2）柱填料的粒径为0.5μm以上且5μm以下; 和（3）无油玻璃，具有上述（1）〜（3）的微滴柱，在电离源中具有这样的微阵列的质谱仪，以及能够使用微喷射柱进行非离子电喷雾的质谱法。
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公开(公告)号：US08507851B2
公开(公告)日：2013-08-13
申请号：US13590033
申请日：2012-08-20
申请人： Toshiaki Isobe , Yoshio Yamauchi
发明人： Toshiaki Isobe , Yoshio Yamauchi
IPC分类号： H01J49/10
CPC分类号： H01J49/10 , G01N30/7266
摘要： Nano-electrospray ionization techniques include the introduction of a separation solvent containing a sample to a column-integrated needle having a column filled with a resin for liquid chromatography. The separated sample components are sprayed from the tip of the column-integrated needle toward a sample introduction orifice of a mass spectrometer. An organic solvent is simultaneously introduced to a solvent-supplying needle. The organic solvent is supplied from the tip of the solvent-supplying needle to the tip of the column-integrated needle.
摘要翻译： 纳米电喷雾电离技术包括将含有样品的分离溶剂引入具有填充有用于液相色谱的树脂的柱的柱一体化针。 将分离的样品组分从柱集成针的尖端喷射到质谱仪的样品引入孔。 将有机溶剂同时引入溶剂供应针。 有机溶剂从溶剂供应针的尖端供应到列一体化针的末端。
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公开(公告)号：US08269165B2
公开(公告)日：2012-09-18
申请号：US12999434
申请日：2010-06-02
申请人： Toshiaki Isobe , Yoshio Yamauchi
发明人： Toshiaki Isobe , Yoshio Yamauchi
IPC分类号： H01J49/10
CPC分类号： H01J49/10 , G01N30/7266
摘要： Nano-electrospray ionization techniques include the introduction of a separation solvent containing a sample to a column-integrated needle having a column filled with a resin for liquid chromatography. The separated sample components are sprayed from the tip of the column-integrated needle toward a sample introduction orifice of a mass spectrometer. An organic solvent is simultaneously introduced to a solvent-supplying needle. The organic solvent is supplied from the tip of the solvent-supplying needle to the tip of the column-integrated needle.
摘要翻译： 纳米电喷雾电离技术包括将含有样品的分离溶剂引入具有填充有用于液相色谱的树脂的柱的柱一体化针。 将分离的样品组分从柱集成针的尖端喷射到质谱仪的样品引入孔。 将有机溶剂同时引入溶剂供应针。 有机溶剂从溶剂供应针的尖端供应到列一体化针的末端。
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公开(公告)号：US20080166696A1
公开(公告)日：2008-07-10
申请号：US10583286
申请日：2004-12-17
IPC分类号： C12Q1/02
CPC分类号： G01N33/6848 , G01N33/6818
摘要： A method for analyzing proteins according to which two types of samples containing proteins are compared using a mass spectrometer, so that the proteins which are included in respective samples are identified and the mass ratio of a protein of the same type that is included in the respective samples is analyzed, wherein the method for analyzing proteins is characterized by including the steps of:respectively digestting said two types of samples containing proteins at portions of a certain amino acid using a restriction enzyme so as to prepare samples containing peptides;modifying peptides which are included in said respective samples containing peptides with labeling compounds having different masses due to isotopes, so that peptides of the same type that are included in the respective samples containing peptides have different masses;mixing the samples containing peptides that have been respectively labeled with isotopes, separating and quantifying the mixed sample for each peptide and measuring the MS spectrum, and finding the content ratio of peptides of the same type having different masses due to isotope labeling;selecting a peptide of which the amino acid sequence should be identified from among the peptides in reference to said MS spectrum and qualitatively analyzing the amino acid sequence of selected peptide from the mass spectrum of the product ions which are generated from the peptide;identifying a corresponding protein from known-DNA sequences on the basis of the amino acid sequence of said peptide; andfinding the ratio of the content of said identified protein included in said samples containing respective proteins on the basis of the value obtained from separation quantification using the difference in the mass of said peptides that have been modified with isotopes.
摘要翻译： 一种用于分析蛋白质的方法，根据该方法，使用质谱仪比较含有蛋白质的两种类型的样品，从而鉴定各个样品中包含的蛋白质，并且包含在相应的样品中的相同类型的蛋白质的质量比 分析样品，其中分析蛋白质的方法的特征在于包括以下步骤：使用限制酶分别在某些氨基酸的部分消化含有蛋白质的所述两种样品，以制备含有肽的样品; 包含在含有由于同位素而具有不同质量的标记化合物的肽的所述各个样品中的肽的肽，使得包含在含有肽的各个样品中的相同类型的肽具有不同的质量; 将含有分别用同位素标记的肽的样品混合，分别定量每个肽的混合样品并测量MS谱，并且发现由于同位素标记而具有不同质量的相同类型的肽的含量比; 从所述MS谱选择肽中应鉴定出氨基酸序列的肽，并从肽生成的产物离子的质谱定性分析所选肽的氨基酸序列; 基于所述肽的氨基酸序列从已知DNA序列鉴定相应的蛋白质; 并且使用已经用同位素修饰的所述肽的质量差异，基于从分离定量获得的值，找到包含在含有各种蛋白质的所述样品中的所述鉴定的蛋白质的含量的比例。
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公开(公告)号：US08401320B2
公开(公告)日：2013-03-19
申请号：US12688705
申请日：2010-01-15
申请人： Mariko Watanabe , Toshiaki Isobe
发明人： Mariko Watanabe , Toshiaki Isobe
IPC分类号： G06K9/46
CPC分类号： H04N1/41
摘要： An image forming apparatus for compressing image data, includes: a compression unit that reduces data into a predetermined bit number per band of image data so as to satisfy a condition related to a predetermined compression and compresses the reduced data; a specifying unit that specifies a minimum bit number among the predetermined bit numbers applied to the bands and specifies bands which are not compressed by the minimum bit number; and a re-compression unit that performs re-compression on the specified bands so that the data is compressed by the minimum bit number.
摘要翻译： 一种用于压缩图像数据的图像形成装置，包括：压缩单元，其将数据减少到每个图像数据的每个频带的预定位数，以满足与预定压缩相关的条件，并压缩缩小的数据; 指定单元，其指定施加到所述频带的预定位数中的最小位数，并指定未被所述最小位数压缩的频带; 以及重新压缩单元，其对指定的频带执行重新压缩，使得数据被最小位数压缩。
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