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公开(公告)号：US08481275B2
公开(公告)日：2013-07-09
申请号：US13509797
申请日：2011-01-17
申请人： Yuka Kobayashi , Ken Kusama , Masugu Kamei
发明人： Yuka Kobayashi , Ken Kusama , Masugu Kamei
IPC分类号： G01N33/53
CPC分类号： G01N33/57438 , G01N2333/375 , G01N2400/00
摘要： To provide a method of accurately detecting pathological haptoglobin using a lectin having strong affinity and high specificity for fucose. The method of the present invention for detecting pancreatic cancer is characterized in that a fucose α1→6 specific lectin is allowed to act on pathological haptoglobin contained in a sample obtained from a living body, said lectin: (1) being extractede from basidiomycetes, (2) having a molecular weight of 4,000 to 40,000 as determined by the SDS polyacrylamide gel electrophoresis, and (3) having affinity for a fucose α1→6 sugar chain with a binding constant of 1.0×104 M−1 or more at 25° C.
摘要翻译： 提供使用对岩藻糖具有强亲和力和高特异性的凝集素来准确检测病理性触珠蛋白的方法。 本发明的检测胰腺癌的方法的特征在于，使岩藻糖α1-6特异性凝集素作用于从活体获得的样品中所含的病理性触珠蛋白，所述凝集素：（1）从担子菌中提取， （2）通过SDS聚丙烯酰胺凝胶电泳测定的分子量为4,000〜40,000，和（3）对于25的结合常数为1.0×10 4 M -1以上的岩藻糖α1-> 6糖链具有亲和力 C。
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2.发明申请公开(公告)号：US20090117599A1
公开(公告)日：2009-05-07
申请号：US12095013
申请日：2006-12-14
CPC分类号： C12Q1/48 , C08B37/00 , G01N33/53 , G01N2333/4724 , G01N2333/91091 , G01N2400/00
摘要： It is intended to provide a method by which sugar chains having GlcNAc transferred by GnT-V can be accurately detected, screened and purified. In this detection method, two kinds of lectins differing in detailed GlcNAc-specificity are used together. As shown in FIG. 1(I), for example, a sample containing sugar chains having GlcNAc transferred by GnT-V is screened by using a lectin GSL-II, which recognizes sugar chains having GlcNAc transferred by GnT-V, and then the screened sample is screened again by using another lectin BLL, which does not recognizes sugar chains having GlcNAc transferred by GnT-V, to thereby detect, screen and purify the sugar chains having GlcNAc transferred by GnT-V alone.
摘要翻译： 旨在提供可以精确地检测，筛选和纯化由GnT-V转移的具有GlcNAc的糖链的方法。 在该检测方法中，两种不同的GlcNAc特异性的凝集素一起使用。 如图所示。 如图1（I）所示，通过使用凝集素GSL-II筛选含有通过GnT-V转移的GlcNAc的糖链的样品，凝集素GSL-II识别通过GnT-V转移的具有GlcNAc的糖链，然后再次筛选筛选的样品 通过使用不识别由GnT-V转移的GlcNAc的糖链的另一种凝集素BLL，从而检测，筛选和纯化仅由GnT-V转移的GlcNAc的糖链。
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公开(公告)号：US20120244563A1
公开(公告)日：2012-09-27
申请号：US13509797
申请日：2011-01-17
申请人： Yuka Kobayashi , Ken Kusama , Masugu Kamei
发明人： Yuka Kobayashi , Ken Kusama , Masugu Kamei
IPC分类号： G01N33/574
CPC分类号： G01N33/57438 , G01N2333/375 , G01N2400/00
摘要： To provide a method of accurately detecting pathological haptoglobin using a lectin having strong affinity and high specificity for fucose. The method of the present invention for detecting pancreatic cancer is characterized in that a fucose α1→6 specific lectin is allowed to act on pathological haptoglobin contained in a sample obtained from a living body, said lectin: (1) being extractede from basidiomycetes, (2) having a molecular weight of 4,000 to 40,000 as determined by the SDS polyacrylamide gel electrophoresis, and (3) having affinity for a fucose α1→6 sugar chain with a binding constant of 1.0×104 M−1 or more at 25° C.
摘要翻译： 提供使用对岩藻糖具有强亲和力和高特异性的凝集素来准确检测病理性触珠蛋白的方法。 本发明的胰腺癌检测方法的特征在于，使岩藻糖α1→6特异性凝集素作用于从生物体获得的样品中所含的病理性触珠蛋白，所述凝集素：（1）从担子菌中提取（ 2）通过SDS聚丙烯酰胺凝胶电泳测定的分子量为4,000〜40,000，（3）对25℃的结合常数为1.0×10 4 M -1以上的岩藻糖α1→6糖链具有亲和性 。
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公开(公告)号：US06319680B1
公开(公告)日：2001-11-20
申请号：US09556682
申请日：2000-04-21
申请人： Shoichi Yasuno , Masugu Kamei , Junko Arai , Keiko Saito
发明人： Shoichi Yasuno , Masugu Kamei , Junko Arai , Keiko Saito
IPC分类号： C12Q134
CPC分类号： C12Q1/34
摘要： A method for the determination of monosaccharides in a sugar composition by simultaneous quantitative analysis of monosaccharides constituting the sugar composition, comprising the steps of: (1) liberating sialic acid from the sugar composition with sialidase or an acid, (2) converting the released sialic acid into N-acylmannosamine with sialic acid aldolase, and (3) acid hydrolyzing the N-acylmannosamine and a sugar residue. The method allows pretreatment in a single reactor and the sugar composition including sialic acid can be obtained by a single analysis by HPLC, etc.
摘要翻译： 一种通过同时定量分析构成糖组合物的单糖来确定糖组合物中单糖的方法，包括以下步骤：（1）用唾液酸酶或酸从糖组合物中释放唾液酸，（2）将释放的唾液酸 用唾液酸醛缩酶将酸酸化成N-酰基甘露糖胺，和（3）酸性水解N-酰基甘露糖胺和糖残基。 该方法允许在单个反应器中进行预处理，并且可以通过HPLC等的单次分析来获得包含唾液酸的糖组合物。
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