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公开(公告)号：US11851705B2
公开(公告)日：2023-12-26
申请号：US16819564
申请日：2020-03-16
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874
摘要： Provided is a method for a two-step multiplex DNA amplification reaction which allows bacterial or fungal DNA analysis without first extracting DNA from the sample, nor without need to enrich microbes by laboratory culture prior to analysis. Without additional DNA purification or analysis, the PCR amplified DNA is administered directly to a microarray designed to interrogate a large panel of meaningful bacteria or fungi as a single multiplex test. Microarray analysis is then performed at ambient temperature, thus enabling substantial simplification of the testing process. It is contemplated that analysis may be conducted on unprocessed and processed leaf wash and similar surface sampling of plant material, cannabis, vegetables, fruit, nuts, spices, grains, other agriculture samples, food samples, or water samples, so as to detect bacterial, yeast, mold or viral, plant or human pathogen contamination.
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公开(公告)号：US11542498B2
公开(公告)日：2023-01-03
申请号：US15916062
申请日：2018-03-08
IPC分类号： C40B60/04 , C12N15/10 , C12Q1/6837 , G01N33/53
摘要： Provided herein is a 3-dimensional lattice microarray system for DNA sequence detection and analysis. The system has a plurality of bifunctional polymer linkers, on one end of which are attached nucleic acid probes where each have a sequence complementary to signature nucleotide sequences in pathogens, plants or animals. The other end of the bifunctional polymer linker is attached to a solid support by non-covalent or covalent means. Each of the nucleic acid probes have terminal thymidine bases at the 5′ and 3′ ends that permit attachment of the probes to the bifunctional polymer linkers. Also provided is a method for fabricating the microarray system by first attaching the bifunctional polymer linkers to the solid support, followed by photochemical coupling of the nucleic probes to the microarray. A customizable microarray kit is provided that contains the solid support, linkers, probes, solvent mixture and instructions to use the kit.
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公开(公告)号：US20190085414A1
公开(公告)日：2019-03-21
申请号：US16158181
申请日：2018-10-11
IPC分类号： C12Q1/6895 , C12Q1/686 , C12Q1/689 , C12Q1/6809 , C12Q1/6853 , C12Q1/6893 , C12Q1/6837 , C12Q1/6806
摘要： Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay.
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公开(公告)号：US11512307B2
公开(公告)日：2022-11-29
申请号：US16157375
申请日：2018-10-11
IPC分类号： C40B60/04 , C12Q1/6837 , C12N15/10 , G01N33/53
摘要： Provided herein is a 3-dimensional lattice microarray system for DNA sequence detection and analysis. The system has a plurality of bifunctional polymer linkers, on one end of which are attached nucleic acid probes where each have a sequence complementary to signature nucleotide sequences in pathogens, plants or animals. The other end of the bifunctional polymer linker is attached to a solid support by non-covalent or covalent means. Each of the nucleic acid probes have terminal thymidine bases at the 5′ and 3′ ends that permit attachment of the probes to the bifunctional polymer linkers. Also provided is a customizable microarray kit is provided that contains the solid support, linkers, probes, solvent mixture and instructions to use the kit.
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公开(公告)号：US20200017925A1
公开(公告)日：2020-01-16
申请号：US16581054
申请日：2019-09-24
IPC分类号： C12Q1/6895 , C12Q1/689 , C12Q1/6893 , C12Q1/686 , C12Q1/6874 , C12Q1/6844 , C12Q1/682 , C12Q1/6837 , C12Q1/6853 , G16B30/00
摘要： Provided herein is an internal standard method for determining copy number of a pathogen DNA in an unpurified nucleic acid sample by using a known copy number of synthetic DNA that shares a consensus region sequence with the pathogen. The sample is subject to two amplification steps using locus-specific primers and fluorescent primers respectively to obtain fluorescent amplicons for the pathogen and synthetic DNA. These are hybridized with immobilized pathogen-specific and synthetic DNA-specific nucleic acid probes and imaged to obtain fluorescent signals for pathogen-specific and synthetic DNA-specific amplicons. Signal intensities are correlated with the known copy number of synthetic DNA to determine copy number of pathogen DNA in the plant. Also described herein is a method to simultaneously quantitate using the above method, copy numbers of both pathogen and plant DNA in a sample.
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公开(公告)号：US20160150810A1
公开(公告)日：2016-06-02
申请号：US14904241
申请日：2014-07-10
申请人： Pamela HUME , Melissa ROSE
发明人： Pamela Hume , Melissa Rose
IPC分类号： A23L1/216 , A23L1/0522
CPC分类号： A23P20/12 , A23L5/11 , A23L19/105 , A23L19/18 , A23L29/219 , A23V2002/00
摘要： The present disclosure relates to aqueous compositions for coating food products such as French fries, comprising a hydroxypropylated, cross-linked tapioca starch which is a hydroxypropyl di-starch phosphate, and an acetylated, high amylose maize starch.
摘要翻译： 本公开涉及用于包衣食品例如炸薯条的含水组合物，其包含羟丙基化交联的木薯淀粉，其为羟丙基二淀粉磷酸酯，以及乙酰化的高直链淀粉玉米淀粉。
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公开(公告)号：US20220333099A1
公开(公告)日：2022-10-20
申请号：US17855067
申请日：2022-06-30
IPC分类号： C12N15/10 , C12Q1/6837 , G01N33/53
摘要： Provided herein is a method for manufacturing a microarray system, for example, 3-dimensional lattice microarray system, for DNA sequence detection and analysis. A solid support, such as a plastic substrate, is contacted with a formulation containing a plurality of nucleic acid probes, a plurality of bifunctional polymer linkers, such as oligothymidine linkers, and a solvent mixture of water and a water-miscible liquid. The bifunctional polymer linkers are attached to the solid support and the water is evaporated. Then the nucleic acid probes are attached to the bifunctional polymer linker.
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公开(公告)号：US20200283845A1
公开(公告)日：2020-09-10
申请号：US16819564
申请日：2020-03-16
IPC分类号： C12Q1/6874
摘要： Provided is a method for a two-step multiplex DNA amplification reaction which allows bacterial or fungal DNA analysis without first extracting DNA from the sample, nor without need to enrich microbes by laboratory culture prior to analysis. Without additional DNA purification or analysis, the PCR amplified DNA is administered directly to a microarray designed to interrogatea large panel of meaningful bacteria or fungi as a single multiplex test. Microarray analysis is then performed at ambient temperature, thus enabling substantial simplification of the testing process. It is contemplated that analysis may be conducted on unprocessed and processed leaf wash and similar surface sampling of plant material, cannabis, vegetables, fruit, nuts, spices, grains, other agriculture samples, food samples, or water samples, so as to detect bacterial, yeast, mold or viral, plant or human pathogen contamination.
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公开(公告)号：US20180251822A1
公开(公告)日：2018-09-06
申请号：US15916036
申请日：2018-03-08
IPC分类号： C12Q1/6818
CPC分类号： C12Q1/6818 , C12Q1/6837 , C12Q1/689 , C12Q1/6895 , C12Q2531/113 , C12Q2549/119 , C12Q2563/107 , C12Q2565/549
摘要： Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay.
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公开(公告)号：US20130139112A1
公开(公告)日：2013-05-30
申请号：US13305174
申请日：2011-11-28
IPC分类号： G06F3/048
CPC分类号： G06F3/0482 , G06Q10/06 , G06Q10/20
摘要： This disclosure describes embodiments of systems and methods for performing inspections of an asset. The systems can include an inspection apparatus that executes a menu directed inspection (MDI) protocol to direct an inspector that performs the inspection. The MDI protocol includes, in one example, reference material that is associated with areas of the asset that the inspector will inspect. This reference material can include data and information (e.g., technical manuals, operating manuals, images, etc.). In one embodiment, the method includes one or more steps for building an inspection tree with inspection points that correspond to the inspection areas on the asset. The method can also comprise steps for assigning or associating the reference material to inspection points, which is then available to the inspector on the inspection apparatus during execution of the MDI protocol.
摘要翻译： 本公开描述了用于执行资产检查的系统和方法的实施例。 系统可以包括执行菜单导向检查（MDI）协议以指导执行检查的检查员的检查装置。 在一个示例中，MDI协议包括与检查者将检查的资产的区域相关联的参考资料。 该参考资料可以包括数据和信息（例如，技术手册，操作手册，图像等）。 在一个实施例中，该方法包括一个或多个步骤，用于构建具有对应于资产上的检查区域的检查点的检验树。 该方法还可以包括用于将参考材料分配或关联到检查点的步骤，检查点在执行MDI协议期间对检查者可用于检查装置。
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