公开(公告)号：US11566256B2

公开(公告)日：2023-01-31

申请号：US16365323

申请日：2019-03-26

申请人： MENDEL BIOTECHNOLOGY, INC.

发明人： Jose Luis Riechmann ,  Robert Creelman ,  Oliver Ratcliffe ,  T. Lynne Reuber ,  Neal I. Gutterson

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N15/82 ,  C12N15/11 ,  C07K14/415 ,  A01H1/02

摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.

公开(公告)号：US10982218B2

公开(公告)日：2021-04-20

申请号：US16196181

申请日：2018-11-20

申请人： Mendel Biotechnology, Inc.

发明人： Roger Canales ,  Shiv Tiwari ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver J. Ratcliffe

IPC分类号： C12N15/82 ,  C07K14/415

摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

公开(公告)号：US10167480B2

公开(公告)日：2019-01-01

申请号：US14076550

申请日：2013-11-11

申请人： Mendel Biotechnology, Inc.

发明人： Roger Canales ,  Shiv Tiwari ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver J. Ratcliffe

IPC分类号： C12N15/82 ,  C07K14/415

摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

公开(公告)号：US10093942B2

公开(公告)日：2018-10-09

申请号：US15239811

申请日：2016-08-17

申请人： Mendel Biotechnology, Inc.

发明人： Jacqueline E. Heard ,  Jose Luis Riechmann ,  Oliver J. Ratcliffe ,  Omaira Pineda ,  T. Lynne Reuber ,  James Z. Zhang ,  Gregory Nadzan

IPC分类号： C12N15/82 ,  C07K14/415

摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference or control plant, including increased plant size, seed size, increased leaf size, lignification, water deprivation tolerance, cold tolerance, or altered flowering time. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.

公开(公告)号：US09856297B2

公开(公告)日：2018-01-02

申请号：US14229574

申请日：2014-03-28

申请人： Mendel Biotechnology, Inc.

发明人： Jacqueline E Heard ,  Jose Luis Riechmann ,  Robert A Creelman ,  Oliver J Ratcliffe ,  Roger D Canales ,  Peter P Repetti ,  Roderick W Kumimoto ,  Neal I Gutterson ,  T. Lynne Reuber ,  Omaira Pineda ,  Cai-Zhong Jiang ,  Karen S Century ,  Luc Adam ,  James Z Zhang ,  Frederick D Hempel ,  Jeffrey M Libby

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8247 ,  C12N15/825 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8275 ,  C12N15/8281 ,  C12N15/8282

摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having improved tolerance to drought, shade, and low nitrogen conditions, as compared to wild-type or reference plants.

公开(公告)号：US20170226528A1

公开(公告)日：2017-08-10

申请号：US15438520

申请日：2017-02-21

申请人： Mendel Biotechnology, Inc.

发明人： Oliver J. Ratcliffe ,  Peter P. Repetti ,  Neal I. Gutterson ,  Robert A. Creelman

IPC分类号： C12N15/82 ,  A01H1/02

CPC分类号： C12N15/8261 ,  A01H1/02 ,  C07K14/415 ,  C12N15/8262 ,  Y02A40/146

摘要： Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention regulate transcription in these plants and have been shown to confer at least one regulatory activity that results in increased size, biomass, growth rate, and/or yield as compared to a control plant.

公开(公告)号：US20170067073A1

公开(公告)日：2017-03-09

申请号：US15239811

申请日：2016-08-17

申请人： Mendel Biotechnology, Inc.

发明人： Jacqueline E. Heard ,  Jose Luis Riechmann ,  Oliver J. Ratcliffe ,  Omaira Pineda ,  T. Lynne Reuber ,  James Z. Zhang ,  Gregory Nadzan

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N15/8214 ,  C12N15/8241 ,  C12N15/8255 ,  C12N15/8267 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8273 ,  Y02A40/146

摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference or control plant, including increased plant size, seed size, increased leaf size, lignification, water deprivation tolerance, cold tolerance, or altered flowering time. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.

摘要翻译： 本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同系物，以及使用多核苷酸和多肽产生与参考或对照植物相比具有有利性质的转基因植物的方法，包括增加的植物大小， 种子大小，叶片大小，木质化，剥水耐受性，耐寒性或开花时间的改变。 与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法，并且也被公开。

公开(公告)号：US10787677B2

公开(公告)日：2020-09-29

申请号：US15973074

申请日：2018-05-07

申请人： Mendel Biotechnology, Inc. ,  Monsanto Technology LLC

发明人： Robert A. Creelman ,  Neal I. Gutterson ,  Oliver J. Ratcliffe ,  T. Lynne Reuber ,  Richard Eric Cerny ,  Kimberly Faye Zobrist Duff ,  Susanne Kjemtrup-Lovelace ,  Robert J. Meister ,  Marie E. Petracek ,  Thomas Ruff ,  Qingzhang Xu

IPC分类号： C12N15/82 ,  C12N15/00 ,  C07K14/415

摘要： Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, greater early season growth, greater canopy coverage, greater stem diameter, greater late season vigor, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C/N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.

公开(公告)号：US20200010844A1

公开(公告)日：2020-01-09

申请号：US16513497

申请日：2019-07-16

申请人： MENDEL BIOTECHNOLOGY, INC.

发明人： Robert A. Creelman ,  Marjorie R. Lundgren ,  Suqin Cai ,  Hans E. Holtan ,  Graham J. Hymus ,  Oliver J. Ratcliffe ,  T. Lynne Reuber ,  Amanda J. Burek ,  Shiv B. Tiwari ,  Luc J. Adam ,  Colleen M. Marion ,  Megan Lau ,  Bonnie R. Brayton ,  Yu Shen

IPC分类号： C12N15/82 ,  C07K14/415

摘要： The application describes producing polynucleotide variants of the AtHB 17 clade members and introducing the mutant variants into plants to improve plant traits. The mutant polynucleotides encode polypeptides that comprise mutations in the EAR motifs.

公开(公告)号：US10273497B2

公开(公告)日：2019-04-30

申请号：US15713497

申请日：2017-09-22

申请人： Mendel Biotechnology, Inc. ,  Monsanto Technology LLC

发明人： Jose Luis Riechmann ,  Oliver J. Ratcliffe ,  T. Lynne Reuber ,  Katherine Krolikowski ,  Jacqueline E. Heard ,  Omaira Pineda ,  Cai-Zhong Jiang ,  Robert A. Creelman ,  Roderick W. Kumimoto ,  Paul S. Chomet

IPC分类号： C12N15/82 ,  C07K14/415

摘要： Polynucleotides incorporated into nucleic acid constructs have been introduced into plants and were ectopically expressed. The encoded polypeptides of the invention have been shown to confer at least one regulatory activity and confer earlier flowering, longer floral organ retention, increased cold tolerance, greater tolerance to water deprivation, altered carbon-nitrogen balance sensing, increased low nitrogen tolerance, and/or increased tolerance to hyperosmotic stress as compared to a control plant.