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    Strong activation domain
    3.
    发明授权
    Strong activation domain 有权

    公开(公告)号：US10982218B2

    公开(公告)日：2021-04-20

    申请号：US16196181

    申请日：2018-11-20

    申请人： Mendel Biotechnology, Inc.

    发明人： Roger Canales ,  Shiv Tiwari ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver J. Ratcliffe

    IPC分类号： C12N15/82 ,  C07K14/415

    摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

    Strong activation domain
    4.
    发明授权
    Strong activation domain 有权

    公开(公告)号：US10167480B2

    公开(公告)日：2019-01-01

    申请号：US14076550

    申请日：2013-11-11

    申请人： Mendel Biotechnology, Inc.

    发明人： Roger Canales ,  Shiv Tiwari ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver J. Ratcliffe

    IPC分类号： C12N15/82 ,  C07K14/415

    摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

    STRONG ACTIVATION DOMAIN
    5.
    发明申请
    STRONG ACTIVATION DOMAIN 审中-公开

    公开(公告)号：US20190153457A1

    公开(公告)日：2019-05-23

    申请号：US16196181

    申请日：2018-11-20

    申请人： Mendel Biotechnology, Inc.

    发明人： Roger Canales ,  Shiv Tiwari ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver J. Ratcliffe

    IPC分类号： C12N15/82 ,  C07K14/415

    摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

    STRONG ACTIVATION DOMAIN
    7.
    发明申请
    STRONG ACTIVATION DOMAIN 审中-公开
    强活动域

    公开(公告)号：US20140068821A1

    公开(公告)日：2014-03-06

    申请号：US14076550

    申请日：2013-11-11

    申请人： Mendel Biotechnology, Inc.

    发明人： Roger Canales ,  Shiv Tiwari ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver J. Ratcliffe

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8216 ,  C07K14/415 ,  C07K2319/00 ,  C12N15/8251 ,  C12N15/8261 ,  Y02A40/146

    摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

    摘要翻译： 从拟南芥蛋白质乙烯响应因子98（AtERF98）中鉴定出新的和强的转录激活结构域。 该领域已经被指定为“EDLL结构域”，并且具有许多高度保守的氨基酸残基，其在来自植物的AtERF98家族的整个成员中发现，包括单子叶植物和雌蕊直系同源物。 当EDLL结构域与植物和酵母的转录因子融合时，显示其具有高度的活性，并且还显示具有与广泛使用的衍生自单纯疱疹的VP16活化区域相当的活化潜力。 当通过序列特异性DNA结合蛋白或通过蛋白质 - 蛋白质相互作用靶向基因启动子时，EDLL结构域也是活性的。 与其他已知的活化结构域（如VP16和GAL4）不同，EDLL结构域的大小相对较小，并且是植物来源的，因此作为在转基因食品作物中应用的强转录激活工具是有利的。