公开(公告)号：US4430433A

公开(公告)日：1984-02-07

申请号：US326275

申请日：1981-12-01

Applicant: Peter M. Hammond ,  Christopher P. Price ,  Michael D. Scawen

Inventor： Peter M. Hammond ,  Christopher P. Price ,  Michael D. Scawen

IPC: C12N9/80 ,  C12R1/39 ,  C12R1/40 ,  C12N1/20

CPC classification number: C12R1/39 ,  C12N9/80 ,  C12R1/40 ,  Y10S435/815 ,  Y10S435/876 ,  Y10S435/877

Abstract: A process for the production of an aryl acylamidase enzyme involves culturing bacteria of one of the strains Pseudomonas fluorescens ATCC 39005 (or suitable mutants or variants thereof) or Pseudomonas putida ATCC 39004 (or suitable mutants or variants thereof) in a culture medium in which the bacterial strains produce aryl acylamidase and collecting the enzyme containing material, generally the cell material. Preferably the resulting cells are then disrupted, especially by enzymatic treatment, and the aryl acylamidase is separated from the other unwanted substances, generally the other cell constituents.Preferably the culture medium contains N-acylaniline, especially N-acetyl aniline. The N-acylaniline may form part of a complex or defined salts medium.

Abstract translation: 制备芳基酰胺酶的方法涉及培养荧光假单胞菌ATCC 39005（或其合适的突变体或变体）或恶臭假单胞菌ATCC39004（或其合适的突变体或变体）之一的细菌，其中培养基中 细菌菌株产生芳基酰胺酶并收集含酶材料，一般为细胞材料。 优选地，所得细胞特别是通过酶处理被破坏，并且将芳基酰基酰胺酶与其它不需要的物质（通常是其它细胞成分）分离。 培养基优选含有N-酰基苯胺，特别是N-乙酰苯胺。 N-酰基苯胺可以形成复合物或定义的盐介质的一部分。

公开(公告)号：US5670333A

公开(公告)日：1997-09-23

申请号：US211682

申请日：1994-07-28

Applicant: Richard M. Alldread ,  David J. Nicholls ,  Michael D. Scawen ,  Tony Atkinson

Inventor： Richard M. Alldread ,  David J. Nicholls ,  Michael D. Scawen ,  Tony Atkinson

IPC: C12N9/04 ,  C12N15/70 ,  C12R1/19 ,  C12R1/645 ,  C12P21/00 ,  C12N15/64 ,  C12N15/67

CPC classification number: C12N9/0006 ,  C12N15/70

Abstract: Expression vector for expressing the E. coli a polypeptide other than E. coli malate dehydrogenase coded for by a DNA coding sequence. The vector includes a DNA coding for the polypeptide and also includes an initiation codon wherein the DNA sequence is operatively linked to an upstream sequence located upstream of the initiation codon and which is capable of controlling expression of the polypeptide. The upstream sequence consists on the 285 base pair sequence defined by SEQ ID NO:3. A process for expressing a polypeptide by culturing a host strain of E. coli transformed with an expression vector of the invention is also provided.

Abstract translation: PCT No.PCT / GB92 / 01897 Sec。 371日期：1994年7月28日 102（e）日期1994年7月28日PCT提交1992年10月16日PCT公布。 出版物WO93 / 08277 日期1994年4月29日用于表达大肠杆菌的表达载体是由DNA编码序列编码的大肠杆菌苹果酸脱氢酶以外的多肽。 载体包括编码多肽的DNA，还包括起始密码子，其中DNA序列可操作地连接到位于起始密码子上游的上游序列，并能够控制多肽的表达。 上游序列由SEQ ID NO：3定义的285碱基对序列组成。 还提供了通过培养用本发明的表达载体转化的大肠杆菌的宿主菌株来表达多肽的方法。

公开(公告)号：US4767670A

公开(公告)日：1988-08-30

申请号：US011328

申请日：1987-01-21

Applicant: Geoffrey B. Cox ,  Anthony Atkinson ,  Peter A. D. Edwardson ,  Michael D. Scawen

Inventor： Geoffrey B. Cox ,  Anthony Atkinson ,  Peter A. D. Edwardson ,  Michael D. Scawen

IPC: B01D15/08 ,  B01J20/286 ,  B01J20/32 ,  B01J41/04 ,  B01J41/08 ,  B01J41/14 ,  C07H21/04 ,  G01N30/88 ,  B32B9/00 ,  B01J20/00

CPC classification number: B01J20/288 ,  B01D15/325 ,  B01D15/361 ,  B01J20/28004 ,  B01J20/286 ,  B01J20/3071 ,  B01J20/3078 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3259 ,  B01J20/3263 ,  B01J20/3285 ,  B01J41/046 ,  B01J41/20 ,  B01J2220/54 ,  Y10T428/2991 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T428/2998

Abstract: A chromatographic packing useful for the separation of oligonucleotides is disclosed. The packing includes an insert porous support particle and a silane bonded phase comprising a weak anion exchange group in close proximity to at least one polar non-ionic group.

Abstract translation: 可用于分离寡核苷酸的色谱填料包括惰性的，优选多孔的支持颗粒，其与非离子基团非常接近的具有弱阴离子交换基团的硅烷键合。

公开(公告)号：US4414327A

公开(公告)日：1983-11-08

申请号：US326276

申请日：1981-12-01

Applicant: Peter M. Hammond ,  Christopher P. Price ,  Michael D. Scawen ,  Anthony Atkinson

Inventor： Peter M. Hammond ,  Christopher P. Price ,  Michael D. Scawen ,  Anthony Atkinson

IPC: C12N9/80 ,  C12Q1/34 ,  C12R1/38 ,  C12R1/39 ,  C12R1/40

CPC classification number: C12R1/39 ,  C12N9/80 ,  C12Q1/34 ,  G01N2333/21 ,  G01N2333/98 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/876 ,  Y10S435/877

Abstract: A method for the estimation of an anilide in which the anilide is first hydrolyzed enzymatically to an aniline and then the quantity of the aniline produced is estimated spectrophotometrically preferably colorimetrically.The hydrolysis of the anilide may be catalyzed by any enzyme of the type, EC 3.5.1.13, known as aryl acylamidases. Preferably enzymes isolated from the cells of Pseudomonas fluorescens ATCC 39005 or Pseudomonas putida ATCC 39004 are employed.The aniline may be analyzed, for example, by conversion to an indamine, an indophenol or an indoaniline, followed by colorimetric analysis of the colored quinone-type compound produced. This conversion may take place in the presence of an oxidizing agent, such as a copper (II) salt, and/or a base, such as ammonia.A diagnostic kit to allow the routine use of the above method is also provided. In one embodiment of the kit, it comprises,(a) an aryl acylamidase, in solution or on a solid support,(b) an organic compound suitable for the conversion of the aniline to an indamine, an indophenol or an indoaniline, preferably in solution, and(c) a base, preferably in solution.The kit may further comprise an oxidizing agent, again preferably in solution.The above method of analysis and diagnostic kit may be particularly useful in the estimation of anilide drugs, such as paracetamol, dissolved in biological fluids.

Abstract translation: 一种估计苯胺酰胺的方法，其中首先将苯胺酰胺酶促水解成苯胺，然后以比色法优选分光光度法测定所生产的苯胺的量。 酰苯胺的水解可以由称为芳基酰基酰胺酶的任何类型的EC 3.5.1.13催化。 优选从荧光假单胞菌ATCC 39005或恶臭假单胞菌ATCC 39004的细胞分离的酶。 苯胺可以通过例如转化成吲哚酚，靛酚或吲哚苯胺进行分析，然后对生成的有色醌类化合物进行比色分析。 该转化可以在氧化剂如铜（II）盐和/或碱如氨的存在下进行。 还提供了允许常规使用上述方法的诊断试剂盒。 在试剂盒的一个实施方案中，其包含（a）溶液或固体支持物上的芳基酰基酰胺酶，（b）适于将苯胺转化为吲哚酚，靛酚或吲哚苯胺的有机化合物，优选在 溶液，和（c）碱，优选在溶液中。 试剂盒还可以进一步包含氧化剂，最好在溶液中。 上述分析和诊断试剂盒的方法可能特别适用于溶解在生物体液中的苯胺类药物，如对乙酰氨基酚。