公开(公告)号：US08809626B2

公开(公告)日：2014-08-19

申请号：US12809695

申请日：2008-12-16

申请人： Petronella Martina Bleeker ,  Christianus Cornelis Nicolaas Van Schie ,  Paul Johan Diergaarde ,  Robert Cornelis Schuurink ,  Michel Albertus Haring ,  Michiel Theodoor Jan De Both

发明人： Petronella Martina Bleeker ,  Christianus Cornelis Nicolaas Van Schie ,  Paul Johan Diergaarde ,  Robert Cornelis Schuurink ,  Michel Albertus Haring ,  Michiel Theodoor Jan De Both

IPC分类号： C12N15/82 ,  C12N5/04 ,  C12N5/14 ,  C12N15/00 ,  C07H21/04 ,  A01H5/00

CPC分类号： C12N15/8223

摘要： Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising an trichome specific promoter and methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.

摘要翻译： 本文提供了Trichome特异性植物启动子。 还提供了转基因细胞和生物体，特别是包含毛状体特异性启动子的植物细胞和植物，以及使用毛发特异性启动子在细胞和生物体中表达核酸序列的方法。

公开(公告)号：US20080127368A1

公开(公告)日：2008-05-29

申请号：US11661758

申请日：2005-09-01

申请人： Michel Albertus Haring ,  Robert Cornelis Schuurink ,  Julian Cornelis Verdonk ,  Arjen J. VanTunen

发明人： Michel Albertus Haring ,  Robert Cornelis Schuurink ,  Julian Cornelis Verdonk ,  Arjen J. VanTunen

IPC分类号： C12N15/82 ,  C12P21/04 ,  C07K16/18 ,  C07K14/00 ,  C12N15/11 ,  C12N15/00 ,  C12Q1/68 ,  C12N5/06 ,  C12N5/04 ,  C12N1/20 ,  C12N1/00 ,  A01H5/00

CPC分类号： C12N15/1135 ,  C07K14/415 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8254 ,  C12N2310/111 ,  C12N2310/14

摘要： The present invention relates to a polypeptide which belongs to the R3R3-type MYB family and which regulates the shikimate pathway towards the production of benzenoids. The shikimate pathway is a biosynthesis pathway through which the three essential aromatic amino acids tyrosine, phenylalanine and tryptophan are synthesized in plants, bacteria and fungi. The present invention provides for the first time a regulatory protein in the shikimate pathway and a means to regulate the biosynthesis of these three essential amino acids which cannot be produced by mammals. At the same time, it opens up the way for the regulation of the biosynthesis of aromatic and non-aromatic compounds which are derived from these essential amino acids. A polypeptide or polynucleotide of the invention may be used in a method for manipulating the transcript levels of the genes of the shikimate pathway towards benzenoids for instance the genes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS), 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), L-phenylalanine ammonia-lyase (PAL) and chorismate mutase (CM).

摘要翻译： 本发明涉及一种属于R3R3型MYB家族的多肽，其调节脐橙通路生产苯并芘。 莽草酸通路是一种生物合成途径，三种必需芳香族氨基酸酪氨酸，苯丙氨酸和色氨酸在植物，细菌和真菌中合成。 本发明首次提供了莽草酸通路中的调节蛋白和调节哺乳动物不能产生的这三种必需氨基酸的生物合成的方法。 同时，它开辟了调控由这些必需氨基酸衍生的芳族和非芳族化合物的生物合成的方式。 本发明的多肽或多核苷酸可用于操作萜品酸通路基因转录成水平的方法，例如3-脱氧-D-阿拉伯 - 庚酮酸7-磷酸合酶（DAHPS）的基因， 5-烯醇 - 丙酮酸莽草酸-3-磷酸合成酶（EPSPS），L-苯丙氨酸氨裂解酶（PAL）和草酸盐变位酶（CM）。