公开(公告)号：US07138496B2

公开(公告)日：2006-11-21

申请号：US10360828

申请日：2003-02-07

申请人： Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

发明人： Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

IPC分类号： C07K16/00 ,  C12P21/08

CPC分类号： C07K16/2866 ,  A61K39/39541 ,  A61K2039/505 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2319/00 ,  A61K2300/00

摘要： Compositions are provided that comprise antibody against membrane proteins such as chemokine receptors. In particular, monoclonal human antibodies against human CXCR4 are provided that are capable of inhibiting HIV infection and chemotaxis in human breast cancer cells. The antibodies can be used as prophylactics or therapeutics to prevent and treat HIV infection and cancer, for screening drugs, and for diagnosing diseases or conditions associated with interactions with chemokine receptors.

摘要翻译： 提供了包含针对膜蛋白如趋化因子受体的抗体的组合物。 特别地，提供能够抑制人乳腺癌细胞中的HIV感染和趋化性的针对人CXCR4的单克隆抗体。 抗体可用作预防或治疗以预防和治疗HIV感染和癌症，用于筛选药物，以及用于诊断与趋化因子受体相互作用相关的疾病或病症。

公开(公告)号：USRE43823E1

公开(公告)日：2012-11-20

申请号：US13300340

申请日：2011-11-18

申请人： Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

发明人： Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

IPC分类号： C12P21/08 ,  C07K16/00 ,  C12P21/04 ,  C12N5/07 ,  C12N5/16 ,  C12N15/00 ,  A61K39/00 ,  A61K39/395

摘要： Compositions are provided that comprise antibody against coreceptors for human immunodeficiency virus such as CCR5 and CXCR4. In particular, monoclonal human antibodies against human CCR5 are provided that bind to CCR5 with high affinity and are capable of inhibiting HIV infection at low concentrations. The antibodies can be used as prophylactics or therapeutics to prevent and treat HIV infection, for screening drugs, and for diagnosing diseases or conditions associated with interactions with HIV coreceptors.

公开(公告)号：US20050123973A1

公开(公告)日：2005-06-09

申请号：US10988485

申请日：2004-11-12

申请人： Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

发明人： Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07K16/28 ,  C12Q1/68 ,  C07K14/705 ,  G01N33/53 ,  G01N33/543

CPC分类号： C07K16/2866 ,  A61K39/39541 ,  A61K2039/505 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2319/00 ,  A61K2300/00

摘要： Methods are provided for selecting proteins, in particular antibodies, against a wide variety of membrane proteins. In one aspect, a method is provided for eliciting monoclonal antibody in a non-human animal administered with a target membrane protein by using a fusion protein as an immunogen that contains a peptide fragment derived from the target membrane protein. The fusion protein can also be used as a ligand for screening a library of proteins for protein-ligand binding pairs in vitro by using mRNA, ribosome, phage, or cell display. The selected proteins can be used for therapeutics and diagnostics targeting the membrane proteins.

摘要翻译： 提供了针对各种膜蛋白质选择蛋白质，特别是抗体的方法。 一方面，提供了通过使用融合蛋白作为包含衍生自靶膜蛋白的肽片段的免疫原，在施用靶膜蛋白的非人动物中引发单克隆抗体的方法。 融合蛋白也可用作通过使用mRNA，核糖体，噬菌体或细胞显示体外筛选蛋白质 - 配体结合对的蛋白质文库的配体。 所选择的蛋白质可用于靶向膜蛋白的治疗和诊断。