公开(公告)号：US20110237647A1

公开(公告)日：2011-09-29

申请号：US13062673

申请日：2008-09-08

申请人： Senji Shirasawa ,  Takahiro Fujimoto ,  Toshiyuki Tsunoda ,  Keiko Doi ,  Midori Koyanagi

发明人： Senji Shirasawa ,  Takahiro Fujimoto ,  Toshiyuki Tsunoda ,  Keiko Doi ,  Midori Koyanagi

IPC分类号： A61K31/713 ,  C12N15/63 ,  C12N5/071 ,  C07H21/02 ,  A61P35/00 ,  A61P37/06

CPC分类号： A61K31/713 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

摘要： The inhibitory RNA for inhibiting the expression of ZFAT gene according to this invention is a siRNA comprising a sense RNA having a base sequence of contiguous 20 to 20 bases, preferably 23 to 27 bases, of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA or a shRNA comprising a double-stranded RNA (dsRNA) with the sense RNA connected to the anti-sense RNA via a loop sequence.The inhibitory RNA for inhibiting the expression of ZFAT gene according to this invention decreases a rate of cell proliferation of cancer cells, etc., induces apoptosis of cells including cancer cells, or inhibits an immunoresponse by inhibiting the expression of the ZFAT gene. Therefore, the inhibitory RNA of this invention is useful for development of molecular target agents particularly for cancer cells or immunosuppressive agents.

摘要翻译： 用于抑制本发明的ZFAT基因表达的抑制性RNA是包含具有ZFAT mRNA连续20至20个碱基，优选23至27个碱基的碱基序列的正义RNA和具有碱基的反义RNA的siRNA 与有义RNA的碱基序列互补的序列或包含通过环序列连接到反义RNA的有义RNA的双链RNA（dsRNA）的shRNA。 根据本发明抑制ZFAT基因表达的抑制性RNA降低了癌细胞等的细胞增殖速度，诱导包括癌细胞在内的细胞的凋亡，或通过抑制ZFAT基因的表达来抑制免疫应答。 因此，本发明的抑制性RNA可用于开发特别用于癌细胞或免疫抑制剂的分子靶因子。

公开(公告)号：US08580562B2

公开(公告)日：2013-11-12

申请号：US13062673

申请日：2008-09-08

申请人： Senji Shirasawa ,  Takahiro Fujimoto ,  Toshiyuki Tsunoda ,  Keiko Doi ,  Midori Koyanagi

发明人： Senji Shirasawa ,  Takahiro Fujimoto ,  Toshiyuki Tsunoda ,  Keiko Doi ,  Midori Koyanagi

IPC分类号： C12N15/85 ,  C12N15/63 ,  C07H21/02 ,  C07H21/04

CPC分类号： A61K31/713 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

摘要： The inhibitory RNA for inhibiting the expression of ZFAT gene according to this invention is a siRNA comprising a sense RNA having a base sequence of contiguous 20 to 20 bases, preferably 23 to 27 bases, of ZFAT mRNA and an anti-sense RNA having a base sequence complementary to the base sequence of the sense RNA or a shRNA comprising a double-stranded RNA (dsRNA) with the sense RNA connected to the anti-sense RNA via a loop sequence.The inhibitory RNA for inhibiting the expression of ZFAT gene according to this invention decreases a rate of cell proliferation of cancer cells, etc., induces apoptosis of cells including cancer cells, or inhibits an immunoresponse by inhibiting the expression of the ZFAT gene. Therefore, the inhibitory RNA of this invention is useful for development of molecular target agents particularly for cancer cells or immunosuppressive agents.

摘要翻译： 用于抑制本发明的ZFAT基因表达的抑制性RNA是包含具有ZFAT mRNA连续20至20个碱基，优选23至27个碱基的碱基序列的正义RNA和具有碱基的反义RNA的siRNA 与有义RNA的碱基序列互补的序列或包含通过环序列连接到反义RNA的有义RNA的双链RNA（dsRNA）的shRNA。 根据本发明抑制ZFAT基因表达的抑制性RNA降低了癌细胞等的细胞增殖速度，诱导包括癌细胞在内的细胞的凋亡，或通过抑制ZFAT基因的表达来抑制免疫应答。 因此，本发明的抑制性RNA可用于开发特别用于癌细胞或免疫抑制剂的分子靶因子。