公开(公告)号：US5679536A

公开(公告)日：1997-10-21

申请号：US504036

申请日：1995-07-19

申请人： Takashi Hayashi ,  Riko Iwata ,  Mitsuo Yamaki

发明人： Takashi Hayashi ,  Riko Iwata ,  Mitsuo Yamaki

IPC分类号： C12Q1/28 ,  G01N33/58 ,  G01N33/53

CPC分类号： C12Q1/28 ,  G01N33/581 ,  Y10S435/962 ,  Y10S436/825 ,  Y10S436/826

摘要： A chemiluminescent method is provided for the analysis of a target. A peroxidase is first bound as a labelling substance to the target by using a target specific binding reagent. The target so labelled is isolated and then reacted with luminol, hydrogen peroxide and an enhancer in an aqueous solvent. The luminescence of light is detected and measured. The aqueous solvent contains at least one protein selected from the group consisting of skim milk and egg albumin. Preferably, the aqueous solvent also contains a non-ionic surfactant such as a polyoxyethylene ether and/or a sugar alcohol such as mannitol.

摘要翻译： 提供化学发光方法用于目标分析。 首先通过使用目标特异性结合试剂将过氧化物酶作为标记物质结合到靶标上。 将如此标记的靶分离，然后在水溶剂中与鲁米诺，过氧化氢和增强剂反应。 检测并测量光的发光。 含水溶剂含有至少一种选自脱脂乳和蛋白清蛋白的蛋白质。 优选地，水性溶剂还含有非离子表面活性剂如聚氧乙烯醚和/或糖醇如甘露糖醇。

公开(公告)号：US5534430A

公开(公告)日：1996-07-09

申请号：US294996

申请日：1994-08-24

申请人： Kenzo Takada ,  Akiko Tochikura ,  Mitsuo Yamaki

发明人： Kenzo Takada ,  Akiko Tochikura ,  Mitsuo Yamaki

IPC分类号： C12N5/08 ,  C12N7/02 ,  C12N5/00 ,  C12N7/01

CPC分类号： C12N7/00 ,  C12N2710/16251

摘要： The present invention relates to a cell strain capable of separating and multiplying an EB virus and derived from human lymphocyte cancer cells and a method of separating and multiplying the EB virus by means of said cell strain. The present cell strain can be applied to the separation and multiplication of the wild type virus present in various places, as well as to the separation and multiplication of the virus produced by homologous recombination etc. The virus newly separated and multiplied can be applied to vaccine etc. For the more efficient separation and multiplication of the virus, several markers can be inserted into the present cell strain.

摘要翻译： 本发明涉及能够分离和繁殖衍生自人淋巴细胞癌细胞的EB病毒的细胞株和通过所述细胞株分离和倍增EB病毒的方法。 本发明的细胞株可用于各种存在的野生型病毒的分离和繁殖，以及通过同源重组等产生的病毒的分离和繁殖等。新分离和繁殖的病毒可以应用于疫苗 为了更有效地分离和增殖病毒，可以将几种标记物插入到本细胞株中。

公开(公告)号：US6146839A

公开(公告)日：2000-11-14

申请号：US930775

申请日：1997-09-26

申请人： Toshihide Morikawa ,  Kiyotaka Kawagoe ,  Hiroshi Izutsu ,  Akifumi Iguchi ,  Mitsuo Yamaki

发明人： Toshihide Morikawa ,  Kiyotaka Kawagoe ,  Hiroshi Izutsu ,  Akifumi Iguchi ,  Mitsuo Yamaki

IPC分类号： G01N33/569 ,  G01N33/571 ,  A61K35/14 ,  A61K39/118 ,  C12Q1/00

CPC分类号： G01N33/56927

摘要： The present invention relates to a Chlamydia pneumoniae antigen comprising protein derived from the outer membrane of Chlamydia pneumoniae, a method for producing a Chlamydia pneumoniae antigen which comprises solubilizing the cytosol and the cytoplasmic membrane of Chlamydia pneumoniae elementary body with an ionic detergent, and then removing the solubilized portion to obtain the residue, a method for measuring an anti-Chlamydia pneumoniae antibody which comprises using the Chlamydia pneumoniae antigen, and a reagent for measuring anti-Chlamydia pneumoniae antibody, said reagent comprising the Chlamydia pneumoniae antigen.In accordance with the present invention, there are provided a Chlamydia pneumoniae antigen which has a high species-specificity, very few clinically problematic false negatives, and few false positives, a method for producing said antigen, a method for measuring an anti-Chlamydia pneumoniae antibody, and a reagent for measuring an anti-Chlamydia pneumoniae antibody.

摘要翻译： PCT No.PCT / JP96 / 00827 Sec。 371日期：1997年9月29日 102（e）1997年9月29日PCT PCT 1996年3月28日PCT公布。 WO96 / 30763 PCT出版物 日期：1996年10月3日本发明涉及包含肺炎衣原体外膜衍生物的肺炎衣原体抗原，本发明涉及肺炎衣原体抗原的制备方法，其特征在于，将肺炎衣原体的细胞溶质和胞质膜与离子性 洗涤剂，然后除去溶解部分以获得残留物，测定包含使用肺炎衣原体抗原的抗肺炎衣原体抗体的方法和用于测量抗肺炎衣原体抗体的试剂，所述试剂包含肺炎衣原体抗原。 根据本发明，提供了肺炎衣原体抗原，其具有高物种特异性，非常少的临床有问题的假阴性，少量假阳性，产生所述抗原的方法，测定抗肺炎衣原体的方法 抗体和用于测量抗肺炎衣原体抗体的试剂。

公开(公告)号：US4468548A

公开(公告)日：1984-08-28

申请号：US341172

申请日：1982-01-20

申请人： Mitsuo Yamaki

发明人： Mitsuo Yamaki

IPC分类号： F24C1/06 ,  F24C15/10 ,  H05B6/06 ,  H05B6/12 ,  H05B9/02

CPC分类号： H05B6/062 ,  F24C1/06 ,  F24C15/101 ,  F24C15/102 ,  H05B6/1263

摘要： A first casing provided with an induction coil and a top plate as a first heater at the top thereof and a second casing provided with an electric heater as a second heater at the top thereof are hinged by a shaft and hinges such that the second casing can be opened and closed with respect to the first casing and can be held upright when the casing is opened. The power supply to the induction coil and the electric heater is commonly performed by a power unit provided in the first casing.

摘要翻译： 第一壳体，其设置有感应线圈和顶板作为其顶部的第一加热器，并且在其顶部设置有作为第二加热器的电加热器的第二壳体由轴铰接并且铰链，使得第二壳体可 相对于第一壳体打开和关闭，并且当壳体打开时可以竖直地保持。 感应线圈和电加热器的电源通常由设置在第一壳体中的动力单元进行。