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公开(公告)号：US20100213074A1
公开(公告)日：2010-08-26
申请号：US12379704
申请日：2009-02-26
申请人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
发明人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
CPC分类号： B01L3/502761 , B01L3/502753 , B01L3/502784 , B01L3/502792 , B01L2300/087 , B01L2300/089 , B01L2400/0415 , G01N1/4055 , G01N33/74 , G01N2001/4061
摘要： A method of extracting hormones from a biological sample using digital microfluidic arrays is provided. Biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in an extraction step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with excellent precision and recovery.
摘要翻译： 提供了一种使用数字微流体阵列从生物样品中提取激素的方法。 将含有激素的生物样品在数字微流体阵列上干燥，通过溶解溶剂裂解，干燥，随后溶解在极性溶剂中，并在萃取步骤中进一步纯化，其中液滴通过一定体积的非极性溶剂输送。 本文公开的方法提供了自动化样品制备方法的显着优点，其能够以优异的精度和恢复从低样品体积中提取激素。
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公开(公告)号：US20180120335A1
公开(公告)日：2018-05-03
申请号：US15853515
申请日：2017-12-22
申请人： Noha Ahmed MOUSA , Mais J. JEBRAIL , Mohamed Omar ABDELGAWAD , Aaron R. WHEELER , Robert Fredric Joseph CASPER
发明人： Noha Ahmed MOUSA , Mais J. JEBRAIL , Mohamed Omar ABDELGAWAD , Aaron R. WHEELER , Robert Fredric Joseph CASPER
CPC分类号： G01N33/74 , B01L3/502761 , B01L3/502784 , B01L2300/087 , B01L2300/089 , B01L2400/0415 , G01N1/4055 , G01N2001/4061
摘要： Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.
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公开(公告)号：US08202736B2
公开(公告)日：2012-06-19
申请号：US12379704
申请日：2009-02-26
申请人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
发明人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
IPC分类号： G01N33/536
CPC分类号： B01L3/502761 , B01L3/502753 , B01L3/502784 , B01L3/502792 , B01L2300/087 , B01L2300/089 , B01L2400/0415 , G01N1/4055 , G01N33/74 , G01N2001/4061
摘要： A method of extracting hormones from a biological sample using digital microfluidic arrays is provided. Biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in an extraction step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with excellent precision and recovery.
摘要翻译： 提供了一种使用数字微流体阵列从生物样品中提取激素的方法。 将含有激素的生物样品在数字微流体阵列上干燥，通过溶解溶剂裂解，干燥，随后溶解在极性溶剂中，并在萃取步骤中进一步纯化，其中液滴通过一定体积的非极性溶剂输送。 本文公开的方法提供了自动化样品制备方法的显着优点，其能够以优异的精度和恢复从低样品体积中提取激素。
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公开(公告)号：US09851365B2
公开(公告)日：2017-12-26
申请号：US13203701
申请日：2010-02-26
申请人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
发明人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
IPC分类号： G01N33/536 , G01N33/74 , B01L3/00 , G01N1/40
CPC分类号： G01N33/74 , B01L3/502761 , B01L3/502784 , B01L2300/087 , B01L2300/089 , B01L2400/0415 , G01N1/4055 , G01N2001/4061
摘要： Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.
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5.发明申请公开(公告)号：US20120085645A1
公开(公告)日：2012-04-12
申请号：US13203701
申请日：2010-02-26
申请人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
发明人： Noha Ahmed Mousa , Mais J. Jebrail , Mohamed Omar Abdelgawad , Aaron R. Wheeler , Robert Fredric Joseph Casper
IPC分类号： G01N33/53 , G01N33/74 , B81B1/00 , G01N27/447
CPC分类号： G01N33/74 , B01L3/502761 , B01L3/502784 , B01L2300/087 , B01L2300/089 , B01L2400/0415 , G01N1/4055 , G01N2001/4061
摘要： Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.
摘要翻译： 提供了使用数字微流体阵列进行液 - 液萃取的方法和装置。 将极性液滴输送到包含基本上非极性溶剂的分离区域，其中非极性杂质可以从极性液滴中提取，同时保持不同的相分离。 在优选的实施方案中，含有激素的生物样品在数字微流体阵列上干燥，通过裂解溶剂裂解，干燥，随后溶解在极性溶剂中，并在分离步骤中进一步纯化，其中液滴通过一定量的非 - 极性溶剂。 本文公开的方法提供了能够以高精度和恢复从低样品体积提取激素的自动化样品制备方法的显着优点。
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