公开(公告)号：US20070190526A1

公开(公告)日：2007-08-16

申请号：US11706547

申请日：2007-02-15

申请人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Robert A. Eickholt ,  Michael E. Mazelis ,  Wenhuas Xie ,  Richard S. Handley ,  Monica A. Bray ,  Michelle L. Mastronardi ,  Elizabeth A. O'Conner ,  Sarada Siripurapu

发明人： Hashem Akhavan-Tafti ,  Renuka de Silva ,  Robert A. Eickholt ,  Michael E. Mazelis ,  Wenhuas Xie ,  Richard S. Handley ,  Monica A. Bray ,  Michelle L. Mastronardi ,  Elizabeth A. O'Conner ,  Sarada Siripurapu

IPC分类号： C12Q1/70 ,  C07H21/02

CPC分类号： C12Q1/6806

摘要： Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.

摘要翻译： 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。