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公开(公告)号：US20070190526A1
公开(公告)日：2007-08-16
申请号：US11706547
申请日：2007-02-15
申请人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
发明人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
CPC分类号： C12Q1/6806
摘要： Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.
摘要翻译： 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。
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公开(公告)号：US20050106576A1
公开(公告)日：2005-05-19
申请号：US10714763
申请日：2003-11-17
申请人： Hashem Akhavan-Tafti , Renuka de Silva , Nicole Cilli , William Cripps , Richard Handley , Elizabeth O'Connor , Lekkala Reddy , Sarada Siripurapu
发明人： Hashem Akhavan-Tafti , Renuka de Silva , Nicole Cilli , William Cripps , Richard Handley , Elizabeth O'Connor , Lekkala Reddy , Sarada Siripurapu
CPC分类号： C07H21/04 , C07B2200/11
摘要： Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.
摘要翻译： 公开了用于结合核酸的固相材料及其使用方法。 这些材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 固相材料包括固体支持部分，其包含选自二氧化硅，玻璃，不溶性合成聚合物的基质和与核酸结合部分连接以吸附和结合核酸的不溶性多糖，所述核酸结合部分（NAB）为 通过可切割的连接部分连接到固体支持部分。 优选的核酸结合部分包含三元或季铵基团。 材料可以是微粒，纤维，珠，膜，试管或微孔的形式，并且还可以包括磁芯部分。 在分离或纯化核酸的方法中公开了使用可切割固体支持物结合核酸的方法。
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3.发明申请Compositions and methods for releasing nucleic acids from solid phase binding materials 审中-公开用于从固相结合材料释放核酸的组合物和方法公开(公告)号：US20050106589A1
公开(公告)日：2005-05-19
申请号：US10891880
申请日：2004-07-15
CPC分类号： C12N15/1006 , C12N15/101 , C12Q1/6823
摘要： Methods of isolating nucleic acids are disclosed comprising binding the nucleic acid to solid phase binding materials and releasing the bound nucleic acid from the solid phase by elution with a novel reagent composition. Compositions feature a high ionic strength buffer or an added hydrophilic organic co-solvent or both. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.
摘要翻译： 公开了分离核酸的方法，其包括将核酸与固相结合材料结合，并通过用新的试剂组合物洗脱从固相中释放结合的核酸。 组合物具有高离子强度缓冲液或添加的亲水性有机共溶剂或两者。 与本发明的方法和组合物一起使用的优选固相材料包含季鎓核酸结合部分。
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公开(公告)号：US07560556B2
公开(公告)日：2009-07-14
申请号：US11072457
申请日：2005-03-04
IPC分类号： C07D221/06 , G01N33/535 , C12Q1/28
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C07D409/04 , C07D409/06 , C07D411/04 , C07D411/06 , C07F9/64 , C07F9/65586 , C12Q1/28 , G01N33/581 , G01N2333/908
摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。
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公开(公告)号：US07247726B2
公开(公告)日：2007-07-24
申请号：US10468257
申请日：2002-12-18
IPC分类号： C02F9/06 , C07D219/02
CPC分类号： C07D409/04 , C07D219/02 , C07D219/04 , C07D219/06 , C07D411/04 , C07F9/64 , C12Q1/28 , G01N33/535 , Y10S435/968
摘要： Novel compounds comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence rapidly by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了包含在一个碳上被两个含硫原子的基团取代的C-C双键的新型化合物。 该化合物可用于通过与过氧化物酶和过氧化物的反应快速产生化学发光的方法和组合物。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。
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公开(公告)号：US06897036B2
公开(公告)日：2005-05-24
申请号：US10163750
申请日：2002-06-06
IPC分类号： C12Q1/28 , G01N33/533 , C12Q1/00 , G01N33/53
CPC分类号： C12Q1/28 , G01N33/533 , Y10S435/968
摘要： Methods of producing fluorescence from fluorogenic substrates reactive with a peroxidase enzyme are disclosed. Use of the methods in assays for peroxidase enzymes, peroxidase-labeled analytes are provided. Fluorogenic compounds, compositions and kits for reaction with peroxidase enzymes are described. Two modes of producing fluorescent compounds are described.
摘要翻译： 公开了从与过氧化物酶反应的荧光底物产生荧光的方法。 提供了在过氧化物酶，过氧化物酶标记的分析物的测定中使用该方法。 描述了与过氧化物酶反应的荧光化合物，组合物和试剂盒。 描述了产生荧光化合物的两种模式。
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公开(公告)号：US08106203B2
公开(公告)日：2012-01-31
申请号：US12476668
申请日：2009-06-02
IPC分类号： C07D219/00 , C12Q1/28
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C07D409/04 , C07D409/06 , C07D411/04 , C07D411/06 , C07F9/64 , C07F9/65586 , C12Q1/28 , G01N33/581 , G01N2333/908
摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。
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公开(公告)号：US07682839B2
公开(公告)日：2010-03-23
申请号：US11724727
申请日：2007-03-16
IPC分类号： G01N33/533 , G01N21/76 , C07K1/10 , C12Q1/28
CPC分类号： G01N33/582 , C07D219/02 , C07D409/04 , G01N33/53 , Y10S435/968 , Y10T436/13 , Y10T436/143333 , Y10T436/145555
摘要： Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
摘要翻译： 提供了使用化学发光标记化合物和化学发光标记的缀合物的方法。 该化合物包含带有环外烯酮二硫缩醛基团的吖啶环，并且还含有允许附着于感兴趣的化合物的标记取代基。 新型化学发光化合物和标记的共轭物方便制备，高度稳定，并根据需要快速生成化学发光。 化合物和缀合物可用于样品中分析物的测定和使用标记的特异性结合对的测定。
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公开(公告)号：US20090246819A1
公开(公告)日：2009-10-01
申请号：US12476668
申请日：2009-06-02
IPC分类号： C12Q1/28
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C07D409/04 , C07D409/06 , C07D411/04 , C07D411/06 , C07F9/64 , C07F9/65586 , C12Q1/28 , G01N33/581 , G01N2333/908
摘要： Methods for generating chemiluminescence rapidly by reaction of at least one compound comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups with a peroxidase enzyme and a peroxide are disclosed. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. Use of the methods provides rapid signal generation, achieving a plateau intensity in under one minute. The substrate can be provided in a composition which demonstrates unexpectedly long storage stability.
摘要翻译： 公开了通过至少一种包含在一个碳上取代的C-C双键的化合物与两个含硫原子的基团与过氧化物酶和过氧化物反应快速产生化学发光的方法。 由此产生的化学发光可用作过氧化物酶或产生过氧化物酶的测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。 该方法的使用提供了快速的信号发生，在一分钟内达到了高原强度。 基材可以提供在显示出意想不到的长的储存稳定性的组合物中。
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公开(公告)号：US20100190271A1
公开(公告)日：2010-07-29
申请号：US12727651
申请日：2010-03-19
IPC分类号： G01N33/58
CPC分类号： G01N33/582 , C07D219/02 , C07D409/04 , G01N33/53 , Y10S435/968 , Y10T436/13 , Y10T436/143333 , Y10T436/145555
摘要： Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
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