公开(公告)号：US08153387B2

公开(公告)日：2012-04-10

申请号：US12490953

申请日：2009-06-24

Applicant: Chun-Cheng Lin ,  Mu-Lin Chen ,  Po-Chiao Lin ,  Yu-Ju Chen ,  Shu-Hua Chen

Inventor： Chun-Cheng Lin ,  Mu-Lin Chen ,  Po-Chiao Lin ,  Yu-Ju Chen ,  Shu-Hua Chen

IPC: G01N33/53

CPC classification number: C07K16/18 ,  C07K17/14 ,  C07K2317/41 ,  C07K2317/52 ,  G01N33/54353

Abstract: A method for immobilizing glycoprotein is disclosed. A surface is provided. Next, a boronic acid is contacted to the surface. The boronic acid is represented as Y—R—B—(OH)2 and includes a boronic acid group (B—(OH)2), a linker R, and a functional group Y, in which the boronic acid is bonded to the surface via the functional group Y. A glycoprotein is contacted to the boronic acid, wherein two spatially adjacent hydroxyl groups of a carbohydrate chain form a boronate ester with the boronic acid group of the boronic acid to immobilize the glycoprotein. The goal of site-specific and covalent protein immobilization without interfering the binding affinity of an antibody to antigen may be thus achieved with the reaction of boronic acid and the carbohydrate chain of Fc region.

Abstract translation: 公开了一种固定糖蛋白的方法。 提供一个表面。 接下来，硼酸与表面接触。 硼酸被表示为Y-R-B-（OH）2，并且包括硼酸基团（B-（OH）2），连接体R和官能团Y，其中硼酸键合到 通过官能团Y表面。糖蛋白与硼酸接触，其中碳水化合物链的两个空间上相邻的羟基与硼酸的硼酸基团形成硼酸酯以固定糖蛋白。 因此可以通过硼酸和Fc区的碳水化合物链的反应来实现位点特异性和共价蛋白固定而不干扰抗体对抗原的结合亲和力的目标。

公开(公告)号：US20100190966A1

公开(公告)日：2010-07-29

申请号：US12490953

申请日：2009-06-24

Applicant: Chun-Cheng LIN ,  Mu-Lin CHEN ,  Po-Chiao LIN ,  Yu-Ju CHEN ,  Shu-Hua CHEN

Inventor： Chun-Cheng LIN ,  Mu-Lin CHEN ,  Po-Chiao LIN ,  Yu-Ju CHEN ,  Shu-Hua CHEN

IPC: C07K16/00 ,  C07K2/00

CPC classification number: C07K16/18 ,  C07K17/14 ,  C07K2317/41 ,  C07K2317/52 ,  G01N33/54353

Abstract: A method for immobilizing glycoprotein is disclosed. A surface is provided. Next, a boronic acid is contacted to the surface. The boronic acid is represented as Y—R—B—(OH)2 and includes a boronic acid group(B—(OH)2), a linker R, and a functional group Y, in which the boronic acid is bonded to the surface via the functional group Y. A glycoprotein is contacted to the boronic acid, wherein two spatially adjacent hydroxyl groups of a carbohydrate chain form a boronate ester with the boronic acid group of the boronic acid to immobilize the glycoprotein. The goal of site-specific and covalent protein immobilization without interfering the binding affinity of an antibody to antigen may be thus achieved with the reaction of boronic acid and the carbohydrate chain of Fc region.

Abstract translation: 公开了一种固定糖蛋白的方法。 提供一个表面。 接下来，硼酸与表面接触。 硼酸被表示为Y-R-B-（OH）2，并且包括硼酸基团（B-（OH）2），连接体R和官能团Y，其中硼酸键合到 通过官能团Y表面。糖蛋白与硼酸接触，其中碳水化合物链的两个空间上相邻的羟基与硼酸的硼酸基团形成硼酸酯以固定糖蛋白。 因此可以通过硼酸和Fc区的碳水化合物链的反应来实现位点特异性和共价蛋白固定而不干扰抗体对抗原的结合亲和力的目标。

公开(公告)号：US07270036B1

公开(公告)日：2007-09-18

申请号：US11486846

申请日：2006-07-17

Applicant: Mu-Lin Chen

Inventor： Mu-Lin Chen

IPC: B25B23/14

CPC classification number: B25B13/463 ,  B25B23/1427

Abstract: This invention relates to a torque ratchet wrench that comprises a wrench body with a handle at one end and a wrench head at the other end, a driving head would be used for connecting with work pieces, a control device being installed inside the wrench head for using to activate the driving head to run with turning of the wrench in one direction and to void the driving head to run with turning of the wrench in the other direction, a mark plate being implemented on the wrench body with notched torque scales, and an indicator being fixed on the wrench head at one end and extending to above the mark plate at the other end. When the wrench body is driving the driving head rotating, the torque value applied on the wrench driving the work piece can be measured from reading the pointing of the indicator to the notched torque scale on the mark plate.

Abstract translation: 本发明涉及一种扭矩棘轮扳手，其包括在一端具有手柄的扳手主体和在另一端的扳手头，驱动头将用于与工件连接，控制装置安装在扳手头内部，用于 使用来使驱动头在一个方向上转动扳手而行驶，并且使驱动头在另一方向上转动扳手而使驱动头无效运行，标记板在扳手主体上以切口扭矩标尺形成，并且 指示器在一端固定在扳手头上，并在另一端延伸到标记板的上方。 当扳手主体驱动驱动头旋转时，可以从驱动工件的扳手上施加的扭矩值从指示器的指示读取到标记板上的切口扭矩标尺。

公开(公告)号：US06415691B1

公开(公告)日：2002-07-09

申请号：US09941209

申请日：2001-08-29

Applicant: Mu Lin Chen

Inventor： Mu Lin Chen

IPC: B25B1346

CPC classification number: B25B13/463

Abstract: A ratchet wrench structure includes a wrench body defining a receiving hole receiving a ratchet wheel and a receiving recess receiving a locking pawl, a resting block, and two elastic members. The locking pawl has a first side formed with locking teeth engaging the engaging teeth of the ratchet wheel, and a second side formed with an arcuate inner face. The resting block has a first end rested on the arcuate resting face of the receiving recess, and a second end formed with a resting end rested on the arcuate inner face of the locking pawl. The two elastic members received in two sides of the resting block, wherein one elastic member is rested on the arcuate resting face of the receiving recess, and the other elastic member is rested on the arcuate inner face of the locking pawl.

Abstract translation: 棘轮扳手结构包括扳手主体，其限定容纳棘轮的容纳孔和容纳锁定爪的容纳凹槽，静止块和两个弹性构件。 锁定爪具有形成有与棘轮的啮合齿啮合的锁定齿的第一侧和形成有弧形内表面的第二侧。 所述搁置块具有搁置在所述接收凹部的弧形搁置面上的第一端，并且形成有搁置在所述锁定爪的弧形内表面上的搁置端的第二端。 两个弹性构件容纳在搁置块的两侧，其中一个弹性构件搁置在容纳凹部的弧形搁置面上，另一个弹性构件搁置在锁定爪的弓形内表面上。