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公开(公告)号：US07691620B2
公开(公告)日：2010-04-06
申请号：US11717004
申请日：2007-03-13
Applicant: Edward Green , Muhammad Javed , Namdar Baghaei-Yazdi
Inventor： Edward Green , Muhammad Javed , Namdar Baghaei-Yazdi
CPC classification number: C12R1/07 , C12N9/0006 , C12P7/065 , C12P7/56 , Y02E50/17
Abstract: Production of ethanol in a thermophilic bacterium can be achieved by transformation of the bacterium with a heterologous gene encoding pyruvate decarboxylase. The bacterium comprises native alcohol dehydrogenase function and does not comprise a gene encoding a functional lactate dehydrogenase. The bacterium may be grown at elevated temperatures to allow the ethanol to be conveniently removed in a vaporized form from the fermentation medium. Traditional mesophilic microorganisms are incapable of growth at the envisioned elevated temperatures.
Abstract translation: 可以通过用编码丙酮酸脱羧酶的异源基因转化细菌来实现嗜热细菌中的乙醇生产。 该细菌包含天然醇脱氢酶功能，并且不包含编码功能性乳酸脱氢酶的基因。 细菌可以在升高的温度下生长，从而可以从发酵培养基中以汽化形式方便地去除乙醇。 传统的嗜温微生物在预想的高温下不能生长。
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公开(公告)号：US20140212543A1
公开(公告)日：2014-07-31
申请号：US14111318
申请日：2012-04-13
Applicant: Warwick John Lywood , John Turner Pinkney , Muhammad Javed , James Mathew Edwards
Inventor： Warwick John Lywood , John Turner Pinkney , Muhammad Javed , James Mathew Edwards
IPC: A23K1/06
CPC classification number: A23K10/38 , A23K20/163 , C12P7/08 , C12P7/14 , Y02E50/16 , Y02P60/873
Abstract: A method for production of an animal feed product comprises: a) partial hydrolysis of a fermentation feedstock or the non-ethanol by-product of a fermentation process performed on a fermentation feedstock, which partial hydrolysis converts non starch polysaccharides to soluble oligomers and monomers; b) fermentation of the soluble oligomers and monomers in the partially hydrolysed feedstock or non-ethanol by-product to produce ethanol; e) recovery of the non-ethanol by-product from the fermentation of step b) to produce an animal feed product more specifically an animal feed product with improved nutritional content. A method for production of an animal feed product comprises: a) partial hydrolosis of the non ethanol by-product of a fermentation process performed on a fermentation feedstock, which partial hydrolysis converts non starch polysaccharides to soluble oligomers and monomers; b) recovery of the partially hydrolysed product from step a), to exclude the soluble oligomers and monomers, to produce an animal feed product, more specifically an animal feed product with improved nutritional content. The methods produce an animal feed product with improved nutritional content by virtue of decreased levels of pentose sugars, increased relative protein concentration, decreased relative fibre concentration, decreased levels of soluble oligomers and monomers or decreased levels of reducing sugars.
Abstract translation: 一种用于生产动物饲料产品的方法包括：a）部分水解发酵原料或在发酵原料上进行的发酵过程的非乙醇副产物，该部分水解将非淀粉多糖转化为可溶性低聚物和单体; b）在部分水解的原料或非乙醇副产物中发酵可溶性低聚物和单体以产生乙醇; e）从步骤b）的发酵回收非乙醇副产物，更具体地产生具有改善的营养成分的动物饲料产品的动物饲料产品。 一种用于生产动物饲料产品的方法包括：a）对发酵原料进行的发酵过程的非乙醇副产物的部分水合作用，其部分水解将非淀粉多糖转化为可溶性低聚物和单体; b）从步骤a）中回收部分水解的产物，以排除可溶性低聚物和单体，以产生动物饲料产品，更具体地，具有改善的营养成分的动物饲料产品。 该方法通过降低戊糖糖水平，相对蛋白质浓度增加，相对纤维浓度降低，可溶性低聚物和单体水平降低或还原糖水平降低，产生具有改善营养含量的动物饲料产品。
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公开(公告)号：US20110020890A1
公开(公告)日：2011-01-27
申请号：US12867037
申请日：2009-02-12
Applicant: Muhammad Javed , Brian S. Hartley , Namdar Baghaei-Yazdi
Inventor： Muhammad Javed , Brian S. Hartley , Namdar Baghaei-Yazdi
IPC: C12P7/06
CPC classification number: C12P7/065 , C12N9/0006 , C12P7/08 , C12P7/10 , C12Y101/01027 , Y02E50/16 , Y02E50/17
Abstract: Fermentation processes for production of ethanol include supplying a thermophilic microorganism lacking lactate dehydrogenase activity with sugars under conditions in which they metabolise them predominantly by the pyruvate-formate lyase pathway. Importantly, the processes also include supplying sufficient glycerol to convert all of the sugars to ethanol. A further embodiment of the invention includes supplying additional glycerol sufficient to convert the exogenous acetate present in biomass hydrolysates into ethanol. Any type of fermentation system can be used for these processes, but a preferred embodiment includes continuous cultures at high temperatures in which ethanol is removed continuously by vacuum evaporation.
Abstract translation: 用于生产乙醇的发酵方法包括在主要由丙酮酸 - 甲酸裂合酶途径代谢的条件下，向糖中提供缺乏乳酸脱氢酶活性的嗜热微生物。 重要的是，该方法还包括提供足够的甘油以将所有的糖转化为乙醇。 本发明的另一个实施方案包括提供足以将存在于生物质水解产物中的外源乙酸酯转化为乙醇的另外的甘油。 任何类型的发酵系统可用于这些方法，但优选的实施方案包括在高温下的连续培养物，其中通过真空蒸发连续除去乙醇。
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公开(公告)号：US20050181492A1
公开(公告)日：2005-08-18
申请号：US11108870
申请日：2005-04-19
Applicant: Muhammad Javed , Fiona Cusdin , Paul Milner , Edward Green
Inventor： Muhammad Javed , Fiona Cusdin , Paul Milner , Edward Green
CPC classification number: C12N9/00 , C12N9/0004 , C12P7/065 , Y02E50/17
Abstract: The present invention relates to the production of ethanol as a product of bacterial fermentation. In particular this invention relates to a novel method of gene inactivation and gene expression based upon homologous recombination.
Abstract translation: 本发明涉及作为细菌发酵产物的乙醇的生产。 特别地，本发明涉及基于同源重组的基因失活和基因表达的新方法。
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公开(公告)号：US20090226992A1
公开(公告)日：2009-09-10
申请号：US12294084
申请日：2007-03-26
Applicant: Muhammad Javed , Namdar Baghaei-Yazdi
Inventor： Muhammad Javed , Namdar Baghaei-Yazdi
CPC classification number: C12P7/065 , C12N9/0008 , Y02E50/17
Abstract: A thermophilic microorganism lacks lactate dehydrogenase activity and preferably contains an active pyruvate formate lyase pathway. The thermophilic microorganism contains a gene encoding an NAD-linked formate dehydrogenase. The gene encoding an NAD-linked formate dehydrogenase is preferably a codon optimised version of the gene encoding a thermostable NAD-linked formate dehydrogenase. DNA constructs allow stable expression of the gene encoding an NAD-linked formate dehydrogenase in the thermophilic microorganism. The DNA constructs are based upon use of an insertion sequence to achieve stable expression or recombination to insert the gene encoding an NAD-linked formate dehydrogenase into the lactate dehydrogenase gene, thus achieving gene knockout and new functionality in a single step. The microorganisms are useful in fermentation of sugars to produce ethanol.
Abstract translation: 嗜热微生物缺乏乳酸脱氢酶活性，优选含有活性丙酮酸甲酸裂合酶途径。 嗜热微生物含有编码NAD连接的甲酸脱氢酶的基因。 编码NAD连接的甲酸脱氢酶的基因优选是编码热稳定的NAD连接的甲酸脱氢酶的基因的密码子优化版本。 DNA构建体允许在嗜热微生物中稳定表达编码NAD连接的甲酸脱氢酶的基因。 DNA构建体基于使用插入序列以实现稳定表达或重组以将编码NAD连接的甲酸脱氢酶的基因插入乳酸脱氢酶基因，从而在单步骤中实现基因敲除和新功能。 微生物可用于糖的发酵以产生乙醇。
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Patent Agency Ranking
公开(公告)号：US20060126608A1
公开(公告)日：2006-06-15
申请号：US11252768
申请日：2005-10-19
Applicant: Ashley Pereira , Muhammad Javed , Chun Lam , Chung-Yan Yuen
Inventor： Ashley Pereira , Muhammad Javed , Chun Lam , Chung-Yan Yuen
IPC: H04L12/50
CPC classification number: G05B13/042 , G05B23/0245 , H04L41/0853 , H04L41/0856 , H04L41/0866 , H04L41/147 , Y04S40/162
Abstract: A method and an apparatus provide intelligent monitoring and maintenance of a system. The method according to one embodiment accesses data relating to functional components of the system; extracts parameter information for functional components of the system, the step of extracting parameter information including performing inferential processing and trend recognition of the data using previous knowledge about the system, and simulating performance of the system using models of the system and previous knowledge about the system; identifies new information about the system present in extracted parameter information; and provides the new information to the step of extracting parameter information, to be used as previous knowledge.
Abstract translation: 一种方法和装置提供系统的智能监控和维护。 根据一个实施例的方法访问与系统的功能组件有关的数据; 提取系统的功能组件的参数信息，提取参数信息的步骤，包括使用先前关于系统的知识来执行数据的推理处理和趋势识别，以及使用系统模型和关于系统的先前知识来模拟系统的性能 ; 识别提取的参数信息中存在的系统的新信息; 并提供新信息提取参数信息的步骤，作为先前的知识使用。
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公开(公告)号：US08192977B2
公开(公告)日：2012-06-05
申请号：US12617177
申请日：2009-11-12
Applicant: Edward Green , Muhammad Javed , Namdar Baghaei-Yazdi
Inventor： Edward Green , Muhammad Javed , Namdar Baghaei-Yazdi
CPC classification number: C12R1/07 , C12N9/0006 , C12P7/065 , C12P7/56 , Y02E50/17
Abstract: This invention relates to ethanol production as a product of bacterial fermentation.
Abstract translation: 本发明涉及作为细菌发酵产物的乙醇生产。
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公开(公告)号：US20070292928A1
公开(公告)日：2007-12-20
申请号：US11717004
申请日：2007-03-13
Applicant: Edward Green , Muhammad Javed , Namdar Baghaei-Yazdi
Inventor： Edward Green , Muhammad Javed , Namdar Baghaei-Yazdi
CPC classification number: C12R1/07 , C12N9/0006 , C12P7/065 , C12P7/56 , Y02E50/17
Abstract: Production of ethanol in a thermophilic bacterium can be achieved by transformation of the bacterium with a heterologous gene encoding pyruvate decarboxylase. The bacterium comprises native alcohol dehydrogenase function and does not comprise a gene encoding a functional lactate dehydrogenase. The bacterium may be grown at elevated temperatures to allow the ethanol to be conveniently removed in a vaporized form from the fermentation medium. Traditional mesophilic microorganisms are incapable of growth at the envisioned elevated temperatures.
Abstract translation: 在嗜热细菌中生产乙醇可以通过用编码丙酮酸脱羧酶的异源基因转化细菌来实现。 该细菌包含天然醇脱氢酶功能，并且不包含编码功能性乳酸脱氢酶的基因。 细菌可以在升高的温度下生长，从而可以从发酵培养基中以汽化形式方便地去除乙醇。 传统的嗜温微生物在预想的高温下不能生长。
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公开(公告)号：US20050106694A1
公开(公告)日：2005-05-19
申请号：US10484035
申请日：2002-07-18
Applicant: Edward Green , Muhammad Javed , Renia Gemmell
Inventor： Edward Green , Muhammad Javed , Renia Gemmell
Abstract: The present invention relates to a bacterium capable of converting sugars into lactic acid or a salt thereof. The invention also relates to a method for producing lactic acid or a salt thereof comprising culturing the bacterium of the present invention. In particular, the present invention provides a thermophilic bacterium capable of converting at least 70% (w/w) of a monosaccharide sugar and a disaccharide sugar into lactic acid or a salt thereof.
Abstract translation: 本发明涉及能够将糖转化为乳酸的细菌或其盐。 本发明还涉及一种生产乳酸或其盐的方法，其包括培养本发明的细菌。 特别地，本发明提供能够将至少70％（w / w）的单糖和二糖转化成乳酸或其盐的嗜热细菌。
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公开(公告)号：US06664076B2
公开(公告)日：2003-12-16
申请号：US09851419
申请日：2001-05-09
Applicant: Edward M. Green , Fiona S. Cusdin , Namdar Baghaei-Yazdi , Muhammad Javed
Inventor： Edward M. Green , Fiona S. Cusdin , Namdar Baghaei-Yazdi , Muhammad Javed
IPC: C12P2100
CPC classification number: C12N15/102 , C12N15/63 , C12N15/75 , C12N15/87
Abstract: Sporulation-deficient variants of thermophilic, facultatively anaerobic, Gram-positive bacteria can be generated using a plasmid transformation system based on a novel method of in vivo methylation. Such bacteria exhibit improved ethanol production-related characteristics.
Abstract translation: 可以使用基于体内甲基化新方法的质粒转化系统产生嗜热，兼性，厌氧，革兰氏阳性菌的孢子形成缺陷型变体。 这种细菌表现出改善的乙醇生产相关特征。
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