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    Phage-dependent superproduction of biologically active protein and peptides
    2.
    发明授权
    Phage-dependent superproduction of biologically active protein and peptides 失效
    噬菌体依赖性生物活性蛋白和肽的超级生产

    公开(公告)号：US06773899B2

    公开(公告)日：2004-08-10

    申请号：US09929918

    申请日：2001-08-15

    申请人： Vitaliy A. Kordyum ,  Iryna Yu. Slavchenko ,  Svitlana I. Chernykh ,  Oleksandr F. Vozianov

    发明人： Vitaliy A. Kordyum ,  Iryna Yu. Slavchenko ,  Svitlana I. Chernykh ,  Oleksandr F. Vozianov

    IPC分类号： C12P2100

    CPC分类号： C12P21/02 ,  C07K14/50 ,  C12N15/63 ,  C12N15/70

    摘要： This invention relates to a method for enhancing the production of biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage &lgr; with or without the targeted gene(s). The targeted genes encoding the biologically active proteins are under the control of a T7 polymerase promoter and the bacteria also are capable of expressing the gene for T7 RNA polymerase. The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by the combination of the high level of expression achieved from the T7 polymerase promoter and by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.

    摘要翻译： 本发明涉及通过感染生产菌株的细菌细胞来增强细菌细胞中生物活性蛋白质和肽的生产的方法，所述细菌细胞含有具有一个或多个靶向基因的质粒，具有或不具有靶基因的噬菌体λ ）。 编码生物活性蛋白的靶基因处于T7聚合酶启动子的控制之下，细菌也能表达T7 RNA聚合酶的基因。 噬菌体增加靶向蛋白质的合成并诱导生产菌株细胞的裂解。 通过结合来自T7聚合酶启动子的高水平表达和通过延长噬菌体裂解发育的培养条件培养生产菌株细胞来实现超级产生。 随着生产菌株的细胞被噬菌体裂解，生物活性蛋白质和肽随后以可溶形式积累在培养基中。

    High bone mass gene of 11q13.3
    3.
    发明授权
    High bone mass gene of 11q13.3 失效
    11q13.3高骨质量基因

    公开(公告)号：US06770461B1

    公开(公告)日：2004-08-03

    申请号：US09544398

    申请日：2000-04-05

    申请人： John P. Carulli ,  Randall D. Little ,  Robert R. Recker ,  Mark L. Johnson

    发明人： John P. Carulli ,  Randall D. Little ,  Robert R. Recker ,  Mark L. Johnson

    IPC分类号： C12P2100

    CPC分类号： C07K14/47 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/505 ,  C07K14/705 ,  C07K14/775

    摘要： The present invention relates to methods and materials used to isolate and detect a high bone mass gene and a corresponding wild-type gene, and mutants thereof. The present invention also relates to the high bone mass gene, the corresponding wild-type gene, and mutants thereof The genes identified in the present invention are implicated in bone development. The invention also provides nucleic acids, including coding sequences, oligonucleotide primers and probes, proteins, cloning vectors, expression vectors, transformed hosts, methods of developing pharmaceutical compositions, methods of identifying molecules involved in bone development, and methods of diagnosing and treating diseases involved in bone development. In preferred embodiments, the present invention is directed to methods for treating, diagnosing and preventing osteoporosis.

    摘要翻译： 本发明涉及用于分离和检测高骨量基因和相应野生型基因的方法和材料及其突变体。 本发明还涉及高骨量基因，相应的野生型基因及其突变体。本发明鉴定的基因涉及骨发育。 本发明还提供核酸，包括编码序列，寡核苷酸引物和探针，蛋白质，克隆载体，表达载体，转化的宿主，开发药物组合物的方法，鉴定涉及骨发育的分子的方法，以及诊断和治疗涉及的疾病的方法 在骨发育。 在优选的实施方案中，本发明涉及治疗，诊断和预防骨质疏松症的方法。

    Transfection, storage and transfer of male germ cells for generation of transgenic species and genetic therapies
    4.
    发明授权
    Transfection, storage and transfer of male germ cells for generation of transgenic species and genetic therapies 失效
    用于产生转基因物种和遗传疗法的雄性生殖细胞的转染，储存和转移

    公开(公告)号：US06734338B1

    公开(公告)日：2004-05-11

    申请号：US09311599

    申请日：1999-05-13

    申请人： Carol W. Readhead ,  Robert Winston

    发明人： Carol W. Readhead ,  Robert Winston

    IPC分类号： C12P2100

    CPC分类号： A01K67/0275 ,  A01K67/027 ,  A01K2217/05 ,  A61K35/12 ,  A61K48/00 ,  C12N5/061 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2799/021 ,  C12N2799/022 ,  C12N2799/027

    摘要： A composition for in vitro and in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A method of genetically altering a vertebrate male germ cell in vivo employs a lentiviral-derived vector. A method of substantially depopulating a vertebrate testis employs a combination of a dose of an alkylating agent, such as busulfan, chlorambucil, cyclophosphamide, melphalan, or ethyl ethanesulfonic acid, and a dose of gamma radiation. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.

    摘要翻译： 用于脊椎动物雄性生殖细胞的体外和体内转染的组合物包含核酸或转基因，以及基因递送系统和任选的保护性内化剂，例如内体裂解剂，病毒或病毒组分，其是 通过细胞与转基因一起内化，并且通过细胞质增强基因转移到雄性生殖细胞的细胞核。 在体内遗传改变脊椎动物雄性生殖细胞的方法采用慢病毒衍生载体。 一种基本上减少脊椎动物睾丸的方法采用剂量的烷化剂如白消安，苯丁酸氮芥，环磷酰胺，美法仑或乙基乙磺酸以及一定剂量的γ辐射的组合。 药物制剂和转移试剂盒利用组合物。 将多核苷酸引入脊椎动物雄性生殖细胞的方法包括向脊椎动物施用组合物。 分离或选择转染细胞的方法利用报道基因，并且用于转染的雄性生殖细胞的施用方法利用已经在体外转染的雄性生殖细胞。

    Methods of refolding proteins
    7.
    发明授权
    Methods of refolding proteins 有权
    重新折叠蛋白质的方法

    公开(公告)号：US06596511B1

    公开(公告)日：2003-07-22

    申请号：US09507289

    申请日：2000-02-18

    申请人： Steven M. Vicik

    发明人： Steven M. Vicik

    IPC分类号： C12P2100

    CPC分类号： C07K1/1133 ,  C07K1/1136 ,  C07K14/495 ,  C07K14/51

    摘要： Methods are disclosed for refolding proteins of the TGF-&bgr; family of proteins. The methods employ as refolding agents one or more compounds which are non-detergent zwitterionic compounds, such as sulfobetaines, substituted pyridines, substituted pyrroles and acid substituted aminocyclohexanes.

    摘要翻译： 公开了用于重折叠蛋白质的TGF-β家族蛋白质的方法。 该方法使用一种或多种非洗涤剂两性离子化合物的化合物作为重折叠剂，例如磺基甜菜碱，取代的吡啶，取代的吡咯和酸取代的氨基环己烷。

    Stem cell inhibitor
    8.
    发明授权
    Stem cell inhibitor 失效
    干细胞抑制剂

    公开(公告)号：US06558925B2

    公开(公告)日：2003-05-06

    申请号：US09151450

    申请日：1998-09-11

    申请人： Gerard Graham ,  Ian Pragnell

    发明人： Gerard Graham ,  Ian Pragnell

    IPC分类号： C12P2100

    CPC分类号： C07K14/523 ,  A61K38/00

    摘要： The present invention provides a Stem Cell Inhibitor (SCI) protein which comprises at least one amino acid alteration from its native form which protein does not significantly aggregate but which retains substantially unaltered stem cell inhibitory activity. The alteration is preferably a conservative subsitution of a charged amino acid residue. Such proteins may be used in treating stem cells in a patient undergoing chemotherapy.

    摘要翻译： 本发明提供了一种干细胞抑制剂（SCI）蛋白质，其包含至少一种其天然形式的氨基酸改变，该蛋白质不显着聚集，但保留基本上未改变的干细胞抑制活性。 改变优选是带电荷的氨基酸残基的保守取代。 这些蛋白质可用于治疗接受化疗的患者的干细胞。

    Synthetic constructs encoding syndecan
    10.
    发明授权
    Synthetic constructs encoding syndecan 失效
    编码syndecan的合成结构

    公开(公告)号：US06531295B1

    公开(公告)日：2003-03-11

    申请号：US08471970

    申请日：1995-06-06

    申请人： Scott Saunders ,  Merton Bernfield ,  Masato Kato

    发明人： Scott Saunders ,  Merton Bernfield ,  Masato Kato

    IPC分类号： C12P2100

    CPC分类号： C07K16/28 ,  A61K38/00 ,  C07K14/4725 ,  C07K14/475 ,  C07K14/495 ,  C07K14/50 ,  C07K14/705 ,  C07K14/78 ,  C07K2319/00 ,  C08B37/0075 ,  C12N15/1138 ,  C12P19/26

    摘要： A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic region between said cytoplasmic and extracellular regions, a protease susceptible cleavage sequence extracellularly adjacent the transmembrane region of the peptide; and at least one glycosylation site for attachment of a heparan sulfate chain to said extracellular region, said glycosylation site comprising a heparan sulfate attachment sequence represented by a formula Xac-Z-Ser-Gly-Ser-Gly, where Xac represents an amino acid residue having an acidic sidechain, and Z represents from 1 to 10 amino acid residues. Additional peptides having this glycosylation site and genetic information useful for preparing a number of variations based on this glycosylation site are also provided.

    摘要翻译： 纯化的哺乳动物蛋白聚糖和编码这种蛋白聚糖的遗传信息，其核心多肽分子量约为30kD至约35kD，并且包含亲水氨基末端胞外区，亲水性羧基末端胞质区，所述胞质之间的跨膜疏水区 和细胞外区域，在肽跨膜区附近胞外邻近的蛋白酶易裂解序列; 以及用于将硫酸乙酰肝素链连接到所述细胞外区域的至少一个糖基化位点，所述糖基化位点包含由式Xac-Z-Ser-Gly-Ser-Gly表示的硫酸乙酰肝素附着序列，其中Xac表示氨基酸残基 具有酸性侧链，Z表示1〜10个氨基酸残基。 还提供了具有这种糖基化位点的附加肽和用于制备基于该糖基化位点的多种变异的遗传信息。