公开(公告)号：US08699777B2

公开(公告)日：2014-04-15

申请号：US12769057

申请日：2010-04-28

申请人： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

发明人： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

IPC分类号： G06K9/00

CPC分类号： G01N33/86

摘要： A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

摘要翻译： 一种对血液样品中含有的血小板计数的方法，所述方法具有以下步骤：将所述样品与适合于与凝血细胞特异性结合的荧光标记物混合; 和用于抑制所述血小板激活的药剂; 将样品（E）引入到具有至少一个透明面的流体室（CF）中; 在静止照明下通过荧光显微镜获取所述样品的至少一个数字图像（IN）; 以及通过图像处理计算机装置（O）计数存在于所述图像或每个图像中的血小板（T）。 用于实施该方法的装置。

公开(公告)号：US08426156B2

公开(公告)日：2013-04-23

申请号：US12763395

申请日：2010-04-20

申请人： Myriam-Laure Cubizolles ,  Marie-Line Cosnier ,  Magalie Faivre ,  Patrick Pouteau

发明人： Myriam-Laure Cubizolles ,  Marie-Line Cosnier ,  Magalie Faivre ,  Patrick Pouteau

IPC分类号： C12Q1/32

CPC分类号： G01N33/54366 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2300/0822 ,  B01L2300/0877

摘要： The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.

摘要翻译： 本发明涉及一种在全血样品中测定催化血浆酶如转氨酶和NADH依赖性酶的方法，通过在微流体室中测量催化的酶促反应中消耗的NADH的荧光的降低 通过NADH依赖性酶。

公开(公告)号：US20110104712A1

公开(公告)日：2011-05-05

申请号：US12763395

申请日：2010-04-20

申请人： Myriam-Laure Cubizolles ,  Marie-Line Cosnier ,  Magali Faivre ,  Patrick Pouteau

发明人： Myriam-Laure Cubizolles ,  Marie-Line Cosnier ,  Magali Faivre ,  Patrick Pouteau

IPC分类号： G01N33/573 ,  C12M1/40

CPC分类号： G01N33/54366 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2300/0822 ,  B01L2300/0877

摘要： The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.

摘要翻译： 本发明涉及一种在全血样品中测定催化血浆酶如转氨酶和NADH依赖性酶的方法，通过在微流体室中测量催化的酶促反应中消耗的NADH的荧光的降低 通过NADH依赖性酶。

公开(公告)号：US20110002526A1

公开(公告)日：2011-01-06

申请号：US12769057

申请日：2010-04-28

申请人： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

发明人： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

IPC分类号： G06K9/78

CPC分类号： G01N33/86

摘要： A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

摘要翻译： 一种对血液样品中含有的血小板计数的方法，所述方法具有以下步骤：将所述样品与适合于与凝血细胞特异性结合的荧光标记物混合; 和用于抑制所述血小板激活的药剂; 将样品（E）引入到具有至少一个透明面的流体室（CF）中; 在静止照明下通过荧光显微镜获取所述样品的至少一个数字图像（IN）; 以及通过图像处理计算机装置（O）计数存在于所述图像或每个图像中的血小板（T）。 用于实施该方法的装置。