公开(公告)号：US08699777B2

公开(公告)日：2014-04-15

申请号：US12769057

申请日：2010-04-28

Applicant: Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

Inventor： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

IPC: G06K9/00

CPC classification number: G01N33/86

Abstract: A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

Abstract translation: 一种对血液样品中含有的血小板计数的方法，所述方法具有以下步骤：将所述样品与适合于与凝血细胞特异性结合的荧光标记物混合; 和用于抑制所述血小板激活的药剂; 将样品（E）引入到具有至少一个透明面的流体室（CF）中; 在静止照明下通过荧光显微镜获取所述样品的至少一个数字图像（IN）; 以及通过图像处理计算机装置（O）计数存在于所述图像或每个图像中的血小板（T）。 用于实施该方法的装置。

公开(公告)号：US08426156B2

公开(公告)日：2013-04-23

申请号：US12763395

申请日：2010-04-20

Applicant: Myriam-Laure Cubizolles ,  Marie-Line Cosnier ,  Magalie Faivre ,  Patrick Pouteau

Inventor： Myriam-Laure Cubizolles ,  Marie-Line Cosnier ,  Magalie Faivre ,  Patrick Pouteau

IPC: C12Q1/32

CPC classification number: G01N33/54366 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2300/0822 ,  B01L2300/0877

Abstract: The present invention relates to a method for assaying catalytic plasma enzymes, such as transaminases and NADH-dependent enzymes, in a sample of whole blood, by measuring, in a microfluidic chamber, the decrease in the fluorescence of NADH consumed during the enzymatic reactions catalyzed by the NADH-dependent enzymes.

Abstract translation: 本发明涉及一种在全血样品中测定催化血浆酶如转氨酶和NADH依赖性酶的方法，通过在微流体室中测量催化的酶促反应中消耗的NADH的荧光的降低 通过NADH依赖性酶。

公开(公告)号：US20110002526A1

公开(公告)日：2011-01-06

申请号：US12769057

申请日：2010-04-28

Applicant: Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

Inventor： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

IPC: G06K9/78

CPC classification number: G01N33/86

Abstract: A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

Abstract translation: 一种对血液样品中含有的血小板计数的方法，所述方法具有以下步骤：将所述样品与适合于与凝血细胞特异性结合的荧光标记物混合; 和用于抑制所述血小板激活的药剂; 将样品（E）引入到具有至少一个透明面的流体室（CF）中; 在静止照明下通过荧光显微镜获取所述样品的至少一个数字图像（IN）; 以及通过图像处理计算机装置（O）计数存在于所述图像或每个图像中的血小板（T）。 用于实施该方法的装置。

公开(公告)号：US20100248278A1

公开(公告)日：2010-09-30

申请号：US12469200

申请日：2009-05-20

Applicant: Patrick Pouteau ,  Magalie Faivre ,  Philippe Peltie ,  Anne Planat-Chretien

Inventor： Patrick Pouteau ,  Magalie Faivre ,  Philippe Peltie ,  Anne Planat-Chretien

IPC: C12Q1/56 ,  C12Q1/02 ,  C12M1/34

CPC classification number: G01N33/86 ,  G01N21/49 ,  G01N21/75 ,  G01N33/4905 ,  G01N2201/06113 ,  G01N2201/12 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/30004 ,  G06T2207/30024

Abstract: A method of characterizing the coagulation or sedimentation dynamics of a fluid such as whole blood, a blood fraction, or blood plasma, the method comprising: illuminating a sample of said fluid with a beam of coherent light; acquiring a time series of images of a speckle pattern generated by interaction between said sample and said spatially coherent light beam; and processing said time series of images; wherein said processing step includes calculating a function representative of the variation in said speckle pattern between two or more images of the series. The invention also provides a device for implementing such a method.

Abstract translation: 表征诸如全血，血液分数或血浆的流体的凝结或沉降动力学的方法，所述方法包括：用相干光束照射所述流体的样品; 获取由所述样本和所述空间相干光束之间的相互作用产生的散斑图案的时间序列; 并处理所述时间序列的图像; 其中所述处理步骤包括计算代表该系列的两个或多个图像之间的所述斑纹图案的变化的函数。 本发明还提供了一种用于实现这种方法的装置。

公开(公告)号：US09494604B2

公开(公告)日：2016-11-15

申请号：US12469200

申请日：2009-05-20

Applicant: Patrick Pouteau ,  Magalie Faivre ,  Philippe Peltie ,  Anne Planat-Chretien

Inventor： Patrick Pouteau ,  Magalie Faivre ,  Philippe Peltie ,  Anne Planat-Chretien

IPC: G01N33/00 ,  B01L3/00 ,  G01N33/86 ,  G01N33/49 ,  G01N21/49 ,  G01N21/75 ,  G06T7/00

CPC classification number: G01N33/86 ,  G01N21/49 ,  G01N21/75 ,  G01N33/4905 ,  G01N2201/06113 ,  G01N2201/12 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/30004 ,  G06T2207/30024

Abstract: A method of characterizing the coagulation or sedimentation dynamics of a fluid such as whole blood, a blood fraction, or blood plasma is provided. The method includes illuminating a sample of the fluid with a beam of coherent light; acquiring a time series of images of a speckle pattern generated by interaction between the sample and the spatially coherent light beam; and processing the time series of images. The processing step includes calculating a function representative of the variation in the speckle pattern between two or more images of the series. The invention also provides a device for implementing such a method.

Abstract translation: 提供了表征全血，血液分数或血浆等流体的凝固或沉降动力学的方法。 该方法包括用相干光束照射流体的样品; 获取由样本和空间相干光束之间的相互作用产生的斑点图案的时间序列; 并处理图像的时间序列。 处理步骤包括计算表示该系列的两个或多个图像之间的散斑图案的变化的函数。 本发明还提供了一种用于实现这种方法的装置。