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13 条记录 (耗时 0.018 秒)

    Methods for solid-phase nucleic acid amplification and sequencing
    6.
    发明授权
    Methods for solid-phase nucleic acid amplification and sequencing 失效
    固相核酸扩增和测序方法

    公开(公告)号：US06277604B1

    公开(公告)日：2001-08-21

    申请号：US09430035

    申请日：1999-10-29

    申请人： Christine Peponnet

    发明人： Christine Peponnet

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  C12Q1/6846 ,  C12Q2565/537 ,  C12Q2525/197

    摘要： Methods for amplifying and sequencing nucleic acids are disclosed. In addition, supports for the solid phase amplification or sequencing of nucleic acids are disclosed. The supports comprise a solid support, a linker arm having functional groups capable of binding to the solid support, and an oligonucleotide bound to the linker arm.

    摘要翻译： 公开了扩增和测序核酸的方法。 此外，公开了用于核酸的固相扩增或测序的载体。 载体包括固体支持物，具有能够结合固体支持物的官能团的连接臂和与连接臂结合的寡核苷酸。

    Method and apparatus for counting thrombocytes
    7.
    发明授权
    Method and apparatus for counting thrombocytes 有权
    计算凝血细胞的方法和装置

    公开(公告)号：US08699777B2

    公开(公告)日：2014-04-15

    申请号：US12769057

    申请日：2010-04-28

    申请人： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

    发明人： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

    IPC分类号： G06K9/00

    CPC分类号： G01N33/86

    摘要： A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

    摘要翻译： 一种对血液样品中含有的血小板计数的方法，所述方法具有以下步骤：将所述样品与适合于与凝血细胞特异性结合的荧光标记物混合; 和用于抑制所述血小板激活的药剂; 将样品（E）引入到具有至少一个透明面的流体室（CF）中; 在静止照明下通过荧光显微镜获取所述样品的至少一个数字图像（IN）; 以及通过图像处理计算机装置（O）计数存在于所述图像或每个图像中的血小板（T）。 用于实施该方法的装置。

    METHOD AND DEVICE FOR ELECTROWETTING GENETIC ANALYSIS
    8.
    发明申请
    METHOD AND DEVICE FOR ELECTROWETTING GENETIC ANALYSIS 审中-公开
    用于电化遗传分析的方法和装置

    公开(公告)号：US20120045798A1

    公开(公告)日：2012-02-23

    申请号：US13131021

    申请日：2009-11-19

    申请人： Dorothee Jary ,  Christine Peponnet ,  Valérie Van Wilder

    发明人： Dorothee Jary ,  Christine Peponnet ,  Valérie Van Wilder

    IPC分类号： C12P19/34 ,  C12M1/40

    CPC分类号： C12Q1/6846 ,  C12Q2565/629 ,  C12Q2537/143

    摘要： It relates to a method for amplifying at least one nucleotide molecule contained in a sample (E) comprising the following successive steps consisting of (a) subjecting said sample (E) to a 1st amplification step on an area (Z1) of a support; (b) bringing by electrowetting at least one portion of the sample (Ea) obtained after step (a) from the area (Z1) onto at least one area (Z2) of said support, distinct from the area (Z1); (c) subjecting the sample (Ea) to a 2nd amplification step on said area (Z2). It also relates to a device which may be applied within the scope of said method.

    摘要翻译： 本发明涉及用于扩增样品（E）中包含的至少一个核苷酸分子的方法，包括以下连续步骤：（a）使所述样品（E）在载体的区域（Z1）上进行第一扩增步骤; （b）通过将从步骤（a）获得的样品（Ea）的至少一部分从区域（Z1）电润湿到不同于区域（Z1）的所述支撑体的至少一个区域（Z2）; （c）使所述样品（Ea）在所述区域（Z2）上进行第二扩增步骤。 它还涉及可以在所述方法的范围内应用的装置。