公开(公告)号：US20150239956A1

公开(公告)日：2015-08-27

申请号：US14408458

申请日：2013-06-27

Applicant: ASAHI KASEI MEDICAL CO., LTD. ,  NOMADIC BIOSCIENCE CO., LTD.

Inventor： Ichiro Koguma ,  Kazuo Okuyama ,  Satoshi Sato

IPC: C07K16/00

CPC classification number: C07K16/00 ,  C07K1/22 ,  C07K16/18 ,  C07K16/30 ,  C07K2317/21 ,  C07K2317/92

Abstract: A method for manufacturing a high-affinity antibody including purifying an antibody to which a treatment of reducing the affinity for an antigen is not applied by a temperature responsive protein A medium, in which dissociation constant (KD value) to an antigen is smaller than the KD value of an antibody purified by an acid elution type protein A medium.

Abstract translation: 一种制备高亲和力抗体的方法，包括纯化抗体，其中抗原抗体的亲和力降低的抗体不通过对抗原的解离常数（KD值）小于 通过酸洗脱型蛋白A培养基纯化的抗体的KD值。

公开(公告)号：US20150191506A1

公开(公告)日：2015-07-09

申请号：US14410616

申请日：2013-06-28

Applicant: ASAHI KASEI MEDICAL CO., LTD. ,  NOMADIC BIOSCIENCE CO., LTD.

Inventor： Kazuo Okuyama ,  Ichiro Koguma ,  Tomokiyo Marumoto ,  Satoshi Sato

IPC: C07K1/22 ,  B01J20/24 ,  C08F16/06 ,  C07K16/00 ,  C07K14/195

CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01D15/3876 ,  B01J20/24 ,  C07K14/195 ,  C07K16/00 ,  C08F16/06 ,  Y10T428/2982

Abstract: It is an object of the present invention to provide an affinity chromatographic adsorbent using temperature-responsive protein A, wherein the adsorbent is capable of improving the culture productivity of the temperature-responsive protein A and the stability of the temperature-responsive protein A in cell disruption solution. According to the present invention, there is provided an adsorbent consisting of a carrier, to which a polypeptide comprising a tag peptide, a linker sequence, and a B-domain mutant derived from protein A from the N-terminal side thereof binds, wherein the linker sequence is an amino acid sequence that does not comprise a Val-Pro-Arg sequence and is composed of 7 to 12 amino acid residues; and the binding property of the B-domain mutant derived from protein A to an immunoglobulin can vary depending on temperature under conditions of pH 5 to 9 and a temperature of lower than 60° C.

Abstract translation: 本发明的目的是提供使用温度敏感性蛋白A的亲和层析吸附剂，其中吸附剂能够提高温度响应性蛋白A的培养生产率和细胞中温度敏感性蛋白A的稳定性 破解解决方案 根据本发明，提供了一种由载体组成的吸附剂，其包含标签肽的多肽，接头序列和从N-末端侧衍生自蛋白A​​的B结构域突变体结合，其中 接头序列是不包含Val-Pro-Arg序列并由7至12个氨基酸残基组成的氨基酸序列; 并且衍生自蛋白A​​的B结构域突变体与免疫球蛋白的结合性质可以根据pH5-9的条件下的温度和低于60℃的温度而变化。