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    Proteins activating pro-phenoloxidase system and genes encoding the same
    4.
    发明授权
    Proteins activating pro-phenoloxidase system and genes encoding the same 有权
    蛋白激活前酚氧化酶系统和编码相同的基因

    公开(公告)号：US08729230B2

    公开(公告)日：2014-05-20

    申请号：US12526482

    申请日：2008-02-04

    申请人： Bok-Luel Lee ,  Ji-Won Park ,  Nam-Chul Ha ,  Chan-Hee Kim ,  Su-Jin Kim ,  Kyung-Baeg Roh

    发明人： Bok-Luel Lee ,  Ji-Won Park ,  Nam-Chul Ha ,  Chan-Hee Kim ,  Su-Jin Kim ,  Kyung-Baeg Roh

    IPC分类号： C07K14/00

    CPC分类号： C07K14/43504

    摘要： The present invention provides novel proteins activating pro-phenoloxidase (pro-PO) system of Tenebrio molitor, genes encoding the same, methods of detecting bacterial infection in a sample using the proteins, and kits for detecting bacterial infection in a sample using the proteins. The present invention also provides a method of preparing a soluble linearized Lys-type pep-tidoglycan (SLPG), useful for a standard substance for the kit.

    摘要翻译： 本发明提供了活化Tenebrio molitor的前酚氧化酶（pro-PO）系统的新型蛋白质，编码Tenebrio molitor的基因，使用蛋白质的样品中检测细菌感染的方法，以及使用蛋白质检测样品中的细菌感染的试剂盒。 本发明还提供了制备用于该试剂盒的标准物质的可溶性线性化Lys型pep-tidoglycan（SLPG）的方法。

    Method of Separating a Peptidoglycan Recognition Protein From a Hemolymph Tenebrio Molitor Larvae
    7.
    发明申请
    Method of Separating a Peptidoglycan Recognition Protein From a Hemolymph Tenebrio Molitor Larvae 有权
    从血淋巴细胞分离器幼虫分离肽聚糖识别蛋白的方法

    公开(公告)号：US20080312408A1

    公开(公告)日：2008-12-18

    申请号：US12160704

    申请日：2006-01-13

    申请人： Bok-Luel Lee ,  Ji-Won Park ,  Byung-Rok Je ,  Nam-Chul Ha

    发明人： Bok-Luel Lee ,  Ji-Won Park ,  Byung-Rok Je ,  Nam-Chul Ha

    IPC分类号： C07K9/00 ,  C07H21/04 ,  C07K1/22

    CPC分类号： C07K1/22 ,  C07K9/001 ,  C07K14/43504

    摘要： Provided is a method of separating a peptidoglycan-recognition protein from the hemolymph of Tenebrio molitor larvae, the method including: (a) preparing an affinity chromatography column by packing a column with a resin bound with at least one synthetic peptidoglycan; (b) applying the hemolymph of Tenebrio molitor larvae to the affinity chromatography column; (c) applying a mobile phase to the affinity chromatography column to obtain a peptidoglycan-recognition protein-containing solution eluted from the affinity chromatography column; and (d) purifying a peptidoglycan-recognition protein from the eluted solution.

    摘要翻译： 本发明提供了一种将聚乙烯吡咯烷酮识别蛋白与泰乐菌幼虫血淋巴分离的方法，该方法包括：（a）通过用至少一种合成肽聚糖结合的树脂填充柱制备亲和色谱柱; （b）将Tenebrio molitor幼虫的血淋巴应用于亲和层析柱; （c）将流动相施加到亲和色谱柱上，以获得从亲和色谱柱洗脱的含有聚肽聚糖识别蛋白的溶液; 和（d）从洗脱液中纯化肽聚糖识别蛋白。

    Thermostable phytase with 2.1 Å . . . crystal structure
    8.
    发明授权
    Thermostable phytase with 2.1 Å . . . crystal structure 失效
    具有2.1Å的热稳定性植酸酶。 。 。 晶体结构

    公开(公告)号：US06808909B2

    公开(公告)日：2004-10-26

    申请号：US10043623

    申请日：2002-01-09

    申请人： Tae Kwang Oh ,  Nam Chul Ha ,  Byung Ha Oh

    发明人： Tae Kwang Oh ,  Nam Chul Ha ,  Byung Ha Oh

    IPC分类号： C12N900

    CPC分类号： C12N9/16

    摘要： The present invention relates to a thermostable enzyme with a 2.1 Å crystal structure of a propeller type comprising six external blades that encompass the outer boundary of the crystal and six internal calcium-binding sites that are embedded inside the above crystal. Each of the six blades comprises 4 or 5 anti-parallel &bgr;-strands, and the six calcium binding sites consist of 3 high-affinity calcium binding sites and 3 low-affinity binding sites, which are involved in the enzyme's thermostability and catalytic activity, respectively. The above-mentioned Ca2+ binding motifs are expected to be utilized in synthesizing highly thermostable proteins and the elucidation of active sites of an enzyme from a three-dimensional structure can help to design new enzymes having those sites with the aid of recent advanced technology of protein engineering.