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    Device for the generation of a drive signal phase-shifted with respect to an external synchronization
    4.
    发明授权
    Device for the generation of a drive signal phase-shifted with respect to an external synchronization 失效
    用于产生相对于外部同步信号相移的驱动信号的装置

    公开(公告)号：US06198321B1

    公开(公告)日：2001-03-06

    申请号：US09326438

    申请日：1999-06-04

    申请人： Nicolas Lebouleux ,  Benoît Marchand ,  Corrine Ianigro ,  Nathalie Dubois

    发明人： Nicolas Lebouleux ,  Benoît Marchand ,  Corrine Ianigro ,  Nathalie Dubois

    IPC分类号： H03L706

    CPC分类号： H03L7/07

    摘要： A device for the generation of a drive signal phase-shifted with respect to an external synchronization signal includes a first digital phase-locked loop to give a reference signal, servo-linked to the external synchronization signal by a current phase among N phases of a high frequency signal. The device includes a second digital phase-locked loop including a measuring circuit to measure the position of an active edge of the drive signal or a derived signal that is delayed with respect to an active edge of the reference signal. The second phase-locked loop also includes a circuit to compute the phase shift to be made and a phase-shift circuit. The measurement circuit includes a circuit for the rough measurement of the position, controlled by a fixed phase of the high frequency signal independent of the present phase of locking in the first loop. The digital computation circuit accounts for this shift between the fixed phase and the present phase. The device applies to circuits for linear signal processing in a monitor.

    摘要翻译： 用于产生相对于外部同步信号相移的驱动信号的装置包括第一数字锁相环，用于给出参考信号，该参考信号通过当前相位被伺服连接到外部同步信号 高频信号。 该装置包括第二数字锁相环，其包括用于测量驱动信号的有效边沿的位置的测量电路或相对于参考信号的有效边沿被延迟的导出信号。 第二锁相环还包括计算要进行的相移的电路和相移电路。 测量电路包括用于粗略测量位置的电路​​，由与第一回路中的当前锁相相独立的高频信号的固定相控制。 数字计算电路解释了固定相位和当前相位之间的这种偏移。 该器件适用于监视器中的线性信号处理电路。

    HUMAN ANTIBODIES THAT HAVE MN BINDING AND CELL ADHESION-NEUTRALIZING ACTIVITY
    8.
    发明申请
    HUMAN ANTIBODIES THAT HAVE MN BINDING AND CELL ADHESION-NEUTRALIZING ACTIVITY 审中-公开
    具有MN结合和细胞粘附中和活性的人抗体

    公开(公告)号：US20100240100A1

    公开(公告)日：2010-09-23

    申请号：US12330504

    申请日：2008-12-08

    申请人： Toshihiko Takeuchi ,  Nathalie Dubois-Stringfellow ,  John E. Murphy ,  Julie Rinkenberger

    发明人： Toshihiko Takeuchi ,  Nathalie Dubois-Stringfellow ,  John E. Murphy ,  Julie Rinkenberger

    IPC分类号： C12P21/00 ,  C07H21/04 ,  C12N15/74 ,  C12N5/10

    CPC分类号： C07K16/3038 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/565 ,  C07K2317/732

    摘要： The invention is composed of monoclonal human MN antibodies or MN antibody fragments that target the GEEDLP repeat within the proteoglycan domain. The proteoglycan domain of the MN cell surface protein contains four of these identical GEEDLP repeats. Binding to the desired epitope is verified by competition ELISA, where ELISA signal can be attenuated by co-incubation with a peptide containing this repeat (PGEEDLPGEEDLP). This inhibition of binding can also be verified using Biacore assays, where binding of desired antibodies to immobilized MN or proteoglycan peptides can be inhibited by the peptide repeat. In addition to binding to the peptide repeat, human anti-MN antibodies can inhibit the cell adhesion of CGL-1 cells to MN coated plastic plates. Human anti-MN antibodies have been used to diagnose and quantify MN expression in cancer cells and tumors using FACS and immunohistochemical methods. An example is also provided where a human anti-MN IgG1 mediates tumor cell lysis though antibody-dependent cell-mediated cytotoxicity. Therefore, these antibodies will be useful for the treatment of cancers in which MN is upregulated or can be useful for the diagnosis of cancers in which MN is upregulated.

    摘要翻译： 本发明由针对蛋白多糖结构域内的GEEDLP重复的单克隆人MN抗体或MN抗体片段组成。 MN细胞表面蛋白的蛋白多糖结构域包含四个这些相同的GEEDLP重复序列。 通过竞争ELISA验证与所需表位的结合，其中ELISA信号可以通过与含有该重复的肽（PGEEDLPGEEDLP）共孵育来减毒。 还可以使用Biacore分析验证这种结合抑制，其中所需抗体与固定化的MN或蛋白聚糖肽的结合可被肽重复抑制。 除了结合肽重复之外，人抗MN抗体可以抑制CGL-1细胞对MN涂覆的塑料板的细胞粘附。 已经使用人抗MN抗体来诊断和定量癌细胞和肿瘤中的MN表达，使用FACS和免疫组织化学方法。 还提供了一种实例，其中人抗MN IgG1通过抗体依赖性细胞介导的细胞毒性介导肿瘤细胞裂解。 因此，这些抗体可用于治疗其中MN被上调的癌症或可用于诊断MN被上调的癌症。

    Human antibodies that have MN binding and cell adhesion-neutralizing activity
    9.
    发明授权
    Human antibodies that have MN binding and cell adhesion-neutralizing activity 有权
    具有MN结合和细胞粘附中和活性的人抗体

    公开(公告)号：US07462696B2

    公开(公告)日：2008-12-09

    申请号：US10273541

    申请日：2002-10-18

    申请人： Toshihiko Takeuchi ,  Nathalie Dubois-Stringfellow ,  John E. Murphy ,  Julie Rinkenberger

    发明人： Toshihiko Takeuchi ,  Nathalie Dubois-Stringfellow ,  John E. Murphy ,  Julie Rinkenberger

    IPC分类号： C07K16/46 ,  C07K16/24

    CPC分类号： C07K16/3038 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/565 ,  C07K2317/732

    摘要： The invention is composed of monoclonal human MN antibodies or MN antibody fragments that target the GEEDLP (SEQ ID NO: 118) repeat within the proteoglycan domain. The proteoglycan domain of the MN cell surface protein contains four of these identical GEEDLP (SEQ ID NO: 118) repeats. Binding to the desired epitope is verified by competition ELISA, where ELISA signal can be attenuated by co-incubation with a peptide containing this repeat (PGEEDLPGEEDLP (SEQ ID NO: 119)). This inhibition of binding can also be verified using Biacore assays, where binding of desired antibodies to immobilized MN or proteoglycan peptides can be inhibited by the peptide repeat. In addition to binding to the peptide repeat, human anti-MN antibodies can inhibit the cell adhesion of CGL-1 cells to MN coated plastic plates. Human anti-MN antibodies have been used to diagnose and quantify MN expression in cancer cells and tumors using FACS and immunohistochemical methods. An example is also provided where a human anti-MN IgG1 mediates tumor cell lysis though antibody-dependent cell-mediated cytotoxicity. Therefore, these antibodies will be useful for the treatment of cancers in which MN is upregulated or can be useful for the diagnosis of cancers in which MN is upregulated.

    摘要翻译： 本发明由针对蛋白多糖结构域内的GEEDLP（SEQ ID NO：118）重复的单克隆人MN抗体或MN抗体片段组成。 MN细胞表面蛋白的蛋白聚糖结构域包含四个这些相同的GEEDLP（SEQ ID NO：118）重复序列。 通过竞争ELISA验证与所需表位的结合，其中ELISA信号可以通过与含有该重复的肽（PGEEDLPGEEDLP（SEQ ID NO：119））共孵育来减毒。 还可以使用Biacore分析验证这种结合抑制，其中所需抗体与固定化的MN或蛋白聚糖肽的结合可被肽重复抑制。 除了结合肽重复之外，人抗MN抗体可以抑制CGL-1细胞对MN涂覆的塑料板的细胞粘附。 已经使用人抗MN抗体来诊断和定量癌细胞和肿瘤中的MN表达，使用FACS和免疫组织化学方法。 还提供了一种实例，其中人抗MN IgG1通过抗体依赖性细胞介导的细胞毒性介导肿瘤细胞裂解。 因此，这些抗体可用于治疗其中MN被上调的癌症或可用于诊断MN被上调的癌症。