公开(公告)号：US20120267258A1

公开(公告)日：2012-10-25

申请号：US13452466

申请日：2012-04-20

申请人： Yukiharu URAOKA ,  Bin Zheng ,  Seigo SUZUKI ,  Hiroya KIRIMURA ,  Masayoshi SEIKE ,  Shigeki IWANAGA ,  Nobuyasu HORI

发明人： Yukiharu URAOKA ,  Bin Zheng ,  Seigo SUZUKI ,  Hiroya KIRIMURA ,  Masayoshi SEIKE ,  Shigeki IWANAGA ,  Nobuyasu HORI

IPC分类号： G01N27/26

CPC分类号： G01N33/5438 ,  G01N27/3275

摘要： In order to provide a method for electrochemically detecting an analyte which can detect an analyte with high detection sensitivity, when detecting an analyte S trapped on a working electrode, a label binding substance 90 in which a labeling substance 93 and a first binding substance 92 which traps the analyte S are at least retained on a support 91 composed of polypeptide is brought into contact with the analyte. Alternatively, a complex containing the analyte S and a label binding substance 290 in which a labeling substance 293 is retained on a binding substance 291 which binds to the analyte S via a modulator 292 is formed. Then, the labeling substance present on the working electrode is electrochemically detected.

摘要翻译： 为了提供用于电化学检测能够检测具有高检测灵敏度的分析物的分析物的方法，当检测到捕获在工作电极上的分析物S时，将标记物质93和第一结合物质92 截留分析物S至少保留在由多肽组成的支撑体91上与分析物接触。 或者，形成包含分析物S和标记结合物质290的复合物，其中标记物质293保留在结合物质291上，结合物质291经由调节器292与分析物S结合。 然后，电化学地检测存在于工作电极上的标记物质。

公开(公告)号：US20120161268A1

公开(公告)日：2012-06-28

申请号：US13338986

申请日：2011-12-28

申请人： Shigeki IWANAGA ,  Seigo SUZUKI ,  Masayoshi SEIKE ,  Nobuyasu HORI ,  Hiroya KIRIMURA

发明人： Shigeki IWANAGA ,  Seigo SUZUKI ,  Masayoshi SEIKE ,  Nobuyasu HORI ,  Hiroya KIRIMURA

IPC分类号： H01L31/0224 ,  H01L31/18

CPC分类号： G01N33/5438 ,  G01N27/305 ,  G01N27/3277

摘要： To provide an electrode and working electrode substrate capable of detecting a sample substance, and a manufacturing method capable of producing the electrode by simply operations, the present invention provides, in a photocurrent detection electrode, an adhesive layer containing linker molecules interposed between an electrode body constituted by a semiconductor for receiving electrons released from a test substance by photoexcitation, and a metal layer constituted by a metal.

摘要翻译： 为了提供能够检测样品物质的电极和工作电极基板，以及通过简单的操作能够制造电极的制造方法，本发明在光电流检测电极中提供粘合剂层，该粘合剂层含有介于电极体 由用于接收通过光激发从测试物质释放的电子的半导体和由金属构成的金属层构成。

公开(公告)号：US20120103836A1

公开(公告)日：2012-05-03

申请号：US13277712

申请日：2011-10-20

申请人： Nobuyasu HORI ,  Hiroya KIRIMURA

发明人： Nobuyasu HORI ,  Hiroya KIRIMURA

IPC分类号： G01N27/416 ,  G01N27/327 ,  G01N27/403 ,  G01N27/30

CPC分类号： G01N27/305 ,  G01N33/5438

摘要： A detection device for detecting a test substance which is capable of detecting a test substance and a sample substance with high sensitivity, an electrode substrate, a working electrode, an inspection tip, a method of detecting a test substance, and a method of detecting a sample substance are provided in which a reflective part (reflective layer) is disposed on the working electrode so as to reflect excitation light emitted from a light source and passing through the working electrode toward the working electrode.

摘要翻译： 一种用于检测能够检测测试物质和具有高灵敏度的样品物质的检测物质的检测装置，电极基板，工作电极，检查头，检测物质的方法以及检测方法 提供了在工作电极上设置有反射部（反射层）的反射物质，以便将从光源发出的激发光反射并通过工作电极朝向工作电极。

公开(公告)号：US09057688B2

公开(公告)日：2015-06-16

申请号：US13277712

申请日：2011-10-20

申请人： Nobuyasu Hori ,  Hiroya Kirimura

发明人： Nobuyasu Hori ,  Hiroya Kirimura

IPC分类号： G01N27/416 ,  G01N27/30 ,  G01N33/543

CPC分类号： G01N27/305 ,  G01N33/5438

摘要： A detection device for detecting a test substance which is capable of detecting a test substance and a sample substance with high sensitivity, an electrode substrate, a working electrode, an inspection tip, a method of detecting a test substance, and a method of detecting a sample substance are provided in which a reflective part (reflective layer) is disposed on the working electrode so as to reflect excitation light emitted from a light source and passing through the working electrode toward the working electrode.

摘要翻译： 一种用于检测能够检测测试物质和具有高灵敏度的样品物质的检测物质的检测装置，电极基板，工作电极，检查头，检测物质的方法以及检测方法 提供了在工作电极上设置有反射部（反射层）的反射物质，以便将从光源发出的激发光反射并通过工作电极朝向工作电极。

公开(公告)号：US20120132543A1

公开(公告)日：2012-05-31

申请号：US13306710

申请日：2011-11-29

申请人： Seigo SUZUKI ,  Masayoshi SEIKE ,  Shigeki IWANAGA ,  Nobuyasu HORI ,  Hiroya KIRIMURA

发明人： Seigo SUZUKI ,  Masayoshi SEIKE ,  Shigeki IWANAGA ,  Nobuyasu HORI ,  Hiroya KIRIMURA

IPC分类号： G01N33/50 ,  G01N27/26

CPC分类号： G01N33/5438

摘要： A sample substance S is captured by a capture substance 10 which is immobilized on a working electrode body 161 to provide a method of electrochemically detecting a sample substance capable of detecting the sample substance with high sensitivity. A complex which includes, on a soluble carrier 21, a sample substance S and a labeled binder 20 that has a modified labeled substance 23 containing a labeled substance 20 bound to modified labeled substance 23 containing a labeled substance 24 and a binder 22 that binds to the sample substance S, formed on the working electrode 161. The soluble carrier 21 is dissolved, and the modified labeled substance 23 is attracted to the working electrode 161.

摘要翻译： 样品物质S被固定在工作电极体161上的捕获物质10捕获，以提供电化学检测能够以高灵敏度检测样品物质的样品物质的方法。 在可溶性载体21上包含样品物质S和标记粘合剂20的复合物，其具有与标记物质20结合的经修饰标记物质23的修饰标记物质23，所述标记物质20与经标记物质24和粘合剂22结合的修饰标记物质23结合， 样品物质S形成在工作电极161上。可溶性载体21溶解，改性标记物23被吸引到工作电极161。

公开(公告)号：US20120048747A1

公开(公告)日：2012-03-01

申请号：US13218113

申请日：2011-08-25

申请人： Masayoshi SEIKE ,  Nobuyasu HORI ,  Seigo SUZUKI ,  Shigeki IWANAGA ,  Hiroya KIRIMURA

发明人： Masayoshi SEIKE ,  Nobuyasu HORI ,  Seigo SUZUKI ,  Shigeki IWANAGA ,  Hiroya KIRIMURA

IPC分类号： G01N27/26

CPC分类号： G01N27/3278 ,  G01N27/745

摘要： In order to provide a method of electrochemically detecting a target substance, a method of electrochemically detecting an analyte, and a detection set which have a theoretical advantage in the measurement sensitivity obtained by a conventional electrochemical detection method using a working electrode with a trapping substance immobilized, can reuse the working electrode, and can detect an analyte regardless of the size thereof, there is provided a method including: attracting the target substance containing a labeling substance in a liquid sample to a working electrode in which a trapping substance for trapping the target substance containing a labeling substance is not present; and electrochemically detecting the target substance containing a labeling substance.

摘要翻译： 为了提供电化学检测目标物质的方法，电化学检测分析物的方法和检测组，其具有通过常规电化学检测方法获得的测量灵敏度的理论上的优势，所述电化学检测方法使用固定有捕获物质的工作电极 可以重复利用工作电极，并且可以检测分析物而不考虑其大小，提供了一种方法，包括：将含有液体样品中的标记物质的目标物质吸引到用于捕获靶的捕获物质的工作电极 不含标签物质的物质不存在; 并电化学检测含有标记物质的目标物质。

公开(公告)号：US09157884B2

公开(公告)日：2015-10-13

申请号：US13218113

申请日：2011-08-25

申请人： Masayoshi Seike ,  Nobuyasu Hori ,  Seigo Suzuki ,  Shigeki Iwanaga ,  Hiroya Kirimura

发明人： Masayoshi Seike ,  Nobuyasu Hori ,  Seigo Suzuki ,  Shigeki Iwanaga ,  Hiroya Kirimura

IPC分类号： G01N27/26 ,  G01N27/327 ,  G01N27/74

CPC分类号： G01N27/3278 ,  G01N27/745

摘要： In order to provide a method of electrochemically detecting a target substance, a method of electrochemically detecting an analyte, and a detection set which have a theoretical advantage in the measurement sensitivity obtained by a conventional electrochemical detection method using a working electrode with a trapping substance immobilized, can reuse the working electrode, and can detect an analyte regardless of the size thereof, there is provided a method including: attracting the target substance containing a labeling substance in a liquid sample to a working electrode in which a trapping substance for trapping the target substance containing a labeling substance is not present; and electrochemically detecting the target substance containing a labeling substance.

摘要翻译： 为了提供电化学检测目标物质的方法，电化学检测分析物的方法和检测组，其具有通过常规电化学检测方法获得的测量灵敏度的理论上的优势，所述电化学检测方法使用固定有捕获物质的工作电极 可以重复利用工作电极，并且可以检测分析物而不考虑其大小，提供了一种方法，包括：将含有液体样品中的标记物质的目标物质吸引到用于捕获靶的捕获物质的工作电极 不含标签物质的物质不存在; 并电化学检测含有标记物质的目标物质。

公开(公告)号：US08920626B2

公开(公告)日：2014-12-30

申请号：US13306710

申请日：2011-11-29

申请人： Seigo Suzuki ,  Masayoshi Seike ,  Shigeki Iwanaga ,  Nobuyasu Hori ,  Hiroya Kirimura

发明人： Seigo Suzuki ,  Masayoshi Seike ,  Shigeki Iwanaga ,  Nobuyasu Hori ,  Hiroya Kirimura

IPC分类号： G01N27/00 ,  G01N33/543

CPC分类号： G01N33/5438

摘要： A sample substance S is captured by a capture substance 10 which is immobilized on a working electrode body 161 to provide a method of electrochemically detecting a sample substance capable of detecting the sample substance with high sensitivity. A complex which includes, on a soluble carrier 21, a sample substance S and a labeled binder 20 that has a modified labeled substance 23 containing a labeled substance 20 bound to modified labeled substance 23 containing a labeled substance 24 and a binder 22 that binds to the sample substance S, formed on the working electrode 161. The soluble carrier 21 is dissolved, and the modified labeled substance 23 is attracted to the working electrode 161.

摘要翻译： 样品物质S被固定在工作电极体161上的捕获物质10捕获，以提供电化学检测能够以高灵敏度检测样品物质的样品物质的方法。 在可溶性载体21上包含样品物质S和标记粘合剂20的复合物，其具有与标记物质20结合的经修饰标记物质23的修饰标记物质23，所述标记物质20与经标记物质24和粘合剂22结合的修饰标记物质23结合， 样品物质S形成在工作电极161上。可溶性载体21溶解，改性标记物23被吸引到工作电极161。