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    METHODS OF REVERSIBLY BINDING A BIOTIN COMPOUND TO A SUPPORT
    1.
    发明申请
    METHODS OF REVERSIBLY BINDING A BIOTIN COMPOUND TO A SUPPORT 审中-公开
    将生物素化合物可逆地结合到支持物的方法

    公开(公告)号：US20080255004A1

    公开(公告)日：2008-10-16

    申请号：US11940914

    申请日：2007-11-15

    Applicant: Axl A. Neurauter ,  Lars Norderhaug ,  Ole Henrik Brekke ,  Pal Songe

    Inventor： Axl A. Neurauter ,  Lars Norderhaug ,  Ole Henrik Brekke ,  Pal Songe

    IPC: C40B50/18

    CPC classification number: G01N33/82 ,  C12M23/20 ,  C12M47/02 ,  C12N13/00 ,  C40B50/14 ,  G01N33/543 ,  G01N33/54326

    Abstract: Methods of reversal of the binding between a biotin compound and a biotin-binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. The strong interaction between streptavidin or avidin-biotin is made much weaker by using a combination of modified streptavidin or avidin and modified biotin like desthiobiotin or a derivative thereof like DSB-X Biotin. A protein, such as an antibody may be biotinylated with the modified biotin. When this protein is isolated by binding the modified biotin to the modified streptavidin or avidin bound to an solid surface, it may be released under very gently and very rapid conditions by addition of free biotin. In contrast to proteins obtained by the prior art release methods the protein obtained using the previously available release methods, the proteins obtained using the methods disclosed herein will maintain their native conformation. Uses of the methods in various procedures including cell detachment procedures and techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also described.

    Abstract translation: 公开了生物素化合物与生物素结合化合物之间的结合的逆转方法。 示出了从链霉抗生物素蛋白（或抗生物素蛋白）包被的载体中可逆释放生物素化部分的方法。 通过使用经修饰的链霉亲和素或抗生物素蛋白和修饰的生物素如脱硫生物素或其衍生物如DSB-X生物素的组合，链霉抗生物素蛋白或抗生物素蛋白 - 生物素之间的强相互作用变得非常弱。 蛋白质，例如抗体可以用修饰的生物素生物素化。 当通过将修饰的生物素结合到与固体表面结合的经修饰的链霉抗生物素蛋白或抗生物素蛋白来分离该蛋白质时，其可以通过添加游离生物素在非常温和和非常快速的条件下释放。 与通过现有技术释放方法获得的蛋白质相比，使用先前可获得的释放方法获得的蛋白质，使用本文公开的方法获得的蛋白质将保持其天然构象。 还描述了在各种方法中使用方法，包括细胞分离程序以及靶蛋白或核酸分子的检测，鉴定，测定，纯化，分离和/或分离技术。

    Polymer Particles
    2.
    发明申请
    Polymer Particles 审中-公开
    聚合物颗粒

    公开(公告)号：US20070299249A1

    公开(公告)日：2007-12-27

    申请号：US10599039

    申请日：2005-03-17

    Applicant: Pal Songe

    Inventor： Pal Songe

    IPC: C07K1/13 ,  C07K14/00

    CPC classification number: B01J20/26 ,  B01J20/265 ,  B01J20/28009 ,  B01J20/3242 ,  B01J20/3265 ,  B01J45/00

    Abstract: A process for covalently binding a tagged protein to a polymer particle comprising: contacting a tagged protein with a chelating agent-polymer particle conjugate wherein said tag comprises at least two histidine residues and at least two lysine residues and said chelating agent is tridentate, tetradentate or pentadentate and comprises at least two carboxyl groups and is coordinated by Co2+ ions, to form particle-chelating agent Co2+ complex: contacting said complex with a carbodiimide; and optionally removing the Co2+ ions.

    Abstract translation: 一种将标记蛋白质与聚合物颗粒共价结合的方法，包括：使标记的蛋白质与螯合剂 - 聚合物颗粒缀合物接触，其中所述标签包含至少两个组氨酸残基和至少两个赖氨酸残基，并且所述螯合剂是三齿的，四齿的或四 戊二酸酯，并且包含至少两个羧基并且被Co 2+ +离子配位以形成颗粒螯合剂Co 2+络合物：使所述络合物与碳二亚胺接触; 并任选地除去Co 2+离子。

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