公开(公告)号：US20050048523A1

公开(公告)日：2005-03-03

申请号：US10766102

申请日：2004-01-27

Applicant: Patrick Schnable ,  Feng Liu ,  Yan Fu

Inventor： Patrick Schnable ,  Feng Liu ,  Yan Fu

IPC: C12N1/15 ,  C12N1/21 ,  C12N15/10 ,  C12N15/62 ,  C12Q1/68 ,  C07H21/04

CPC classification number: C12N15/1086 ,  C07K2319/21 ,  C12N15/1065 ,  C12N15/62

Abstract: Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5′ or 3′ to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3′ of the ATG start codon and 5′ of the 3-frame His-tag DNA sequence.

Abstract translation: 提供了用于鉴定包含开放阅读框架的核苷酸片段的组合物和方法。 组合物包含在三个可能的阅读框中的每一个中编码ATG起始密码子和组氨酸标签的核苷酸序列，以及包含这种核苷酸序列的载体。 可以向载体提供用于将感兴趣的5'或3'的核苷酸序列插入3框架His标签DNA序列的克隆位点。 载体还提供有用于插入ATG起始密码子的3'和3框His标签DNA序列的5'的感兴趣的核苷酸序列的克隆位点。

公开(公告)号：US20050268352A1

公开(公告)日：2005-12-01

申请号：US11167856

申请日：2005-06-27

Applicant: Basil Nikolau ,  Eve Wurtele ,  David Oliver ,  Robert Behal ,  Patrick Schnable ,  Jinshan Ke ,  Jerry Johnson ,  Carolyn Allred ,  Beth Fatland ,  Isabelle Lutziger ,  Tsui-Jung Wen

Inventor： Basil Nikolau ,  Eve Wurtele ,  David Oliver ,  Robert Behal ,  Patrick Schnable ,  Jinshan Ke ,  Jerry Johnson ,  Carolyn Allred ,  Beth Fatland ,  Isabelle Lutziger ,  Tsui-Jung Wen

IPC: A01H1/00 ,  C12N5/04 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N15/113 ,  C12N15/82

CPC classification number: C12N15/1137 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/8243

Abstract: The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.

Abstract translation: 本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂解酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。 本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织， 其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。 理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。 此外，本发明提供了包含编码丙酮酸脱羧酶（PDC），pPDH的E1alpha亚基，pPDH的E1beta亚基，pPDH的E2亚基，线粒体丙酮酸脱氢酶（mtPDH）的核酸序列的反义序列的重组载体， 或醛脱氢酶（ALDH）或可以切割编码PDC，E1alpha pPDH，E 1βpPDH，E2 pPDH，mtPDH或ALDH的RNA分子的核酶。