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    Sugar-containing hydrogel for immobilization
    4.
    发明授权
    Sugar-containing hydrogel for immobilization 有权
    含糖水凝胶用于固定

    公开(公告)号：US07785770B2

    公开(公告)日：2010-08-31

    申请号：US11444819

    申请日：2006-05-19

    Applicant: Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

    Inventor： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

    IPC: C12Q1/00

    CPC classification number: G01N33/548 ,  G01N33/54353

    Abstract: The use of sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. Unusually large sizes of interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. Sugar-containing hydrogels have extremely low non-specific absorption of labeled target molecules, providing low background levels. Some hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. Additionally, they nearly always experience non-specific absorption of labeled target molecules, limiting their usefulness in sensor applications. A method is provided for preparing sugar polyacrylate hydrogels with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

    Abstract translation: 使用含糖水凝胶作为用于固定寡核苷酸，肽，蛋白质，抗原，抗体，多糖和其他用于传感器应用的生物分子的非常高度多孔的水性支持材料。 异常大尺寸的相互连通的孔允许大的目标分子快速通过并通过凝胶并结合固定化的生物分子。 含糖水凝胶对标记的靶分子具有极低的非特异性吸收，提供低的背景水平。 一些水凝胶材料不具有这种类型的均匀相互连接的大孔隙度，因此大的靶分子不能容易地通过它们扩散。 此外，它们几乎总是经历对标记的靶分子的非特异性吸收，限制了它们在传感器应用中的用途。 提供了一种制备具有共价键合寡核苷酸和肽的功能化学基团的糖聚丙烯酸酯水凝胶的方法。 还提供了将丙烯酸酯封端的寡核苷酸与糖丙酯单体和二丙烯酸酯交联剂共聚的方法。

    Immobilization of oligonucleotides and proteins in sugar-containing hydrogels
    6.
    发明授权
    Immobilization of oligonucleotides and proteins in sugar-containing hydrogels 有权
    寡糖和蛋白质在含糖水凝胶中的固定

    公开(公告)号：US07785769B2

    公开(公告)日：2010-08-31

    申请号：US10627143

    申请日：2003-07-25

    Applicant: Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

    Inventor： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

    IPC: C12Q1/00

    CPC classification number: G01N33/548 ,  G01N33/54353

    Abstract: The use of sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. Unusually large sizes of interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. Sugar-containing hydrogels have extremely low non-specific absorption of labeled target molecules, providing low background levels. Some hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. Additionally, they nearly always experience non-specific absorption of labeled target molecules, limiting their usefulness in sensor applications. A method is provided for preparing sugar polyacrylate hydrogels with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

    Abstract translation: 使用含糖水凝胶作为用于固定寡核苷酸，肽，蛋白质，抗原，抗体，多糖和其他用于传感器应用的生物分子的非常高度多孔的水性载体材料。 异常大尺寸的相互连通的孔允许大的目标分子快速通过并通过凝胶并结合固定化的生物分子。 含糖水凝胶对标记的靶分子具有极低的非特异性吸收，提供低的背景水平。 一些水凝胶材料不具有这种类型的均匀相互连接的大孔隙度，因此大的靶分子不能容易地通过它们扩散。 此外，它们几乎总是经历对标记的靶分子的非特异性吸收，限制了它们在传感器应用中的用途。 提供了一种制备具有共价键合寡核苷酸和肽的功能化学基团的糖聚丙烯酸酯水凝胶的方法。 还提供了将丙烯酸酯封端的寡核苷酸与糖丙酯单体和二丙烯酸酯交联剂共聚的方法。

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