Method of forming multicellular spheroids from the cultured cells
    1.
    发明申请
    Method of forming multicellular spheroids from the cultured cells 审中-公开
    从培养细胞形成多细胞球体的方法

    公开(公告)号:US20070148767A1

    公开(公告)日:2007-06-28

    申请号:US11318882

    申请日:2005-12-28

    IPC分类号: C12N5/08 C12N5/06

    摘要: Provided herein are methods for producing multicellular spheroids or embryoid bodies suitable for providing cells in large scale for various medical applications. In one embodiment, a method of forming embryoid bodies is provided, which comprises culturing undifferentiated HES cells in a culture vessel pre-coated with cellulose and/or its derivatives. In another embodiment, a method of forming hepatic spheroids is provided, which comprises culturing hepatocytes in a culture vessel pre-coated with cellulose and/or its derivatives.

    摘要翻译: 本文提供了适用于大规模提供用于各种医疗应用的细胞的多细胞球体或胚状体的方法。 在一个实施方案中,提供了形成胚状体的方法,其包括在预先包被纤维素和/或其衍生物的培养容器中培养未分化的HES细胞。 在另一个实施方案中,提供了形成肝球体的方法,其包括在预先涂有纤维素和/或其衍生物的培养容器中培养肝细胞。

    Living cell sheet
    2.
    发明申请
    Living cell sheet 审中-公开
    活细胞片

    公开(公告)号:US20070092493A1

    公开(公告)日:2007-04-26

    申请号:US11256729

    申请日:2005-10-24

    IPC分类号: A61K35/12 C12N5/08

    CPC分类号: C12N5/0068 C12N2539/10

    摘要: A novel method, using a thermoreversible MC/PBS/Collagen hydrogel coated on the TCPS dish, for harvesting a living cell sheet with ECM. The coated hydrogel system is reusable and can be used for culturing a multi-layer cell sheet. The obtained living cell sheets may be used for tissue reconstructions.

    摘要翻译: 一种新颖的方法,使用涂覆在TCPS皿上的热可逆MC / PBS /胶原水凝胶,用ECM收集活细胞片。 涂覆的水凝胶体系是可重复使用的,可用于培养多层细胞片。 获得的活细胞片可以用于组织重建。

    Nanoparticles for protein drug delivery
    7.
    发明授权
    Nanoparticles for protein drug delivery 有权
    用于蛋白质药物递送的纳米颗粒

    公开(公告)号:US07993624B2

    公开(公告)日:2011-08-09

    申请号:US12931202

    申请日:2011-01-26

    IPC分类号: A61K9/50 A61K51/00

    摘要: The invention discloses nanoparticles composed of chitosan, poly-glutamic acid, and at least one bioactive agent characterized with a positive surface charge. The bioactive agent is hydrophobic or lipophilic in nature and is associated with micelles before being encapsulated in nanoparticles.

    摘要翻译: 本发明公开了由壳聚糖,聚谷氨酸和至少一种以正表面电荷为特征的生物活性剂组成的纳米颗粒。 生物活性剂本质上是疏水性或亲脂性的,并且在包封在纳米颗粒中之前与胶束相关。