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公开(公告)号:US20070148767A1
公开(公告)日:2007-06-28
申请号:US11318882
申请日:2005-12-28
申请人: Mei-Ju Yang , Wann-Hsin Chen , Pei-Ju Lin , Chun-Hung Chen , Hsing-Wen Sung
发明人: Mei-Ju Yang , Wann-Hsin Chen , Pei-Ju Lin , Chun-Hung Chen , Hsing-Wen Sung
CPC分类号: C12N5/0068 , C12N5/0606 , C12N5/067 , C12N2533/78
摘要: Provided herein are methods for producing multicellular spheroids or embryoid bodies suitable for providing cells in large scale for various medical applications. In one embodiment, a method of forming embryoid bodies is provided, which comprises culturing undifferentiated HES cells in a culture vessel pre-coated with cellulose and/or its derivatives. In another embodiment, a method of forming hepatic spheroids is provided, which comprises culturing hepatocytes in a culture vessel pre-coated with cellulose and/or its derivatives.
摘要翻译: 本文提供了适用于大规模提供用于各种医疗应用的细胞的多细胞球体或胚状体的方法。 在一个实施方案中,提供了形成胚状体的方法,其包括在预先包被纤维素和/或其衍生物的培养容器中培养未分化的HES细胞。 在另一个实施方案中,提供了形成肝球体的方法,其包括在预先涂有纤维素和/或其衍生物的培养容器中培养肝细胞。
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公开(公告)号:US20070092493A1
公开(公告)日:2007-04-26
申请号:US11256729
申请日:2005-10-24
申请人: Hsing-Wen Sung , Chun-Hung Chen , Hosheng Tu
发明人: Hsing-Wen Sung , Chun-Hung Chen , Hosheng Tu
CPC分类号: C12N5/0068 , C12N2539/10
摘要: A novel method, using a thermoreversible MC/PBS/Collagen hydrogel coated on the TCPS dish, for harvesting a living cell sheet with ECM. The coated hydrogel system is reusable and can be used for culturing a multi-layer cell sheet. The obtained living cell sheets may be used for tissue reconstructions.
摘要翻译: 一种新颖的方法,使用涂覆在TCPS皿上的热可逆MC / PBS /胶原水凝胶,用ECM收集活细胞片。 涂覆的水凝胶体系是可重复使用的,可用于培养多层细胞片。 获得的活细胞片可以用于组织重建。
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公开(公告)号:US08535640B1
公开(公告)日:2013-09-17
申请号:US13776111
申请日:2013-02-25
申请人: Hsing-Wen Sung , Ha Giang Thi Nguyen , Fang Yi Su , Li-Wen Hsu , Er-Yuan Chung , Hosheng Tu
发明人: Hsing-Wen Sung , Ha Giang Thi Nguyen , Fang Yi Su , Li-Wen Hsu , Er-Yuan Chung , Hosheng Tu
IPC分类号: A61K51/00
CPC分类号: A61K31/722 , A61K9/0007 , A61K9/0019 , A61K9/0024 , A61K9/19 , A61K9/4808 , A61K9/4891 , A61K9/5161 , A61K38/00 , A61K47/6935 , A61K47/6939 , A61K49/0043 , A61K49/0093 , B82Y5/00
摘要: The invention discloses a composition of nanoparticles composed of positively charged chitosan, one negatively charged substrate, and optionally a zero-charge compound or at least one tumor necrosis factor inhibitor for oral delivery. The chitosan-based nanoparticles are characterized with a positive surface charge and enhanced permeability for oral drug delivery.
摘要翻译: 本发明公开了一种由带正电荷的壳聚糖,一种带负电荷的底物和任选的零电荷化合物或至少一种用于口服递送的肿瘤坏死因子抑制剂组成的纳米颗粒组合物。 基于壳聚糖的纳米颗粒的特征在于具有正的表面电荷和增强的口服药物递送的渗透性。
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公开(公告)号:US08349799B1
公开(公告)日:2013-01-08
申请号:US13525307
申请日:2012-06-16
申请人: Hsing-Wen Sung , Kiran Sonaje , Ho-Ngoc Nguyen , Er-Yuan Chuang , Hosheng Tu
发明人: Hsing-Wen Sung , Kiran Sonaje , Ho-Ngoc Nguyen , Er-Yuan Chuang , Hosheng Tu
IPC分类号: A61K38/00
CPC分类号: A61K31/722 , A61K9/0019 , A61K9/19 , A61K9/4808 , A61K9/4891 , A61K9/5161 , A61K38/00 , A61K47/6935 , A61K47/6939 , A61K49/0043 , A61K49/0093 , B82Y5/00
摘要: The invention discloses a pharmaceutical composition of bioactive nanoparticles composed of chitosan, poly-glutamic acid, and a bioactive agent for oral delivery. The chitosan-based nanoparticles are characterized with a positive surface charge and enhanced permeability for drug delivery.
摘要翻译: 本发明公开了一种由壳聚糖,聚谷氨酸和用于口服递送的生物活性剂组成的生物活性纳米粒子的药物组合物。 基于壳聚糖的纳米颗粒的特征在于具有正的表面电荷并增加药物递送的渗透性。
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公开(公告)号:US08287905B1
公开(公告)日:2012-10-16
申请号:US13438764
申请日:2012-04-03
申请人: Hsing-Wen Sung , Er-Yuan Chuang , Ha Giang Thi Nguyen , Fang Yi Su , Kiran Sonaje , Hosheng Tu
发明人: Hsing-Wen Sung , Er-Yuan Chuang , Ha Giang Thi Nguyen , Fang Yi Su , Kiran Sonaje , Hosheng Tu
IPC分类号: A61K9/66
CPC分类号: A61K9/5161 , A61K9/0019 , A61K9/19 , A61K9/4808 , A61K9/4891 , A61K38/00 , A61K47/6935 , A61K47/6939
摘要: The invention discloses a composition of chitosan-shelled nanoparticles and methods of manufacturing. The chitosan-shelled nanoparticles are characterized with a positive surface charge and enhanced epithelial permeability for oral drug delivery.
摘要翻译: 本发明公开了一种壳聚糖壳形纳米颗粒的组成及其制造方法。 壳聚糖壳的纳米粒子的特征在于具有正的表面电荷和增强的口服药物递送的上皮通透性。
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公开(公告)号:US08257740B1
公开(公告)日:2012-09-04
申请号:US13136912
申请日:2011-08-15
申请人: Hsing-Wen Sung , Kiran Sonaje , Ho-Ngoc Nguyen , Hosheng Tu
发明人: Hsing-Wen Sung , Kiran Sonaje , Ho-Ngoc Nguyen , Hosheng Tu
IPC分类号: A61K9/14
CPC分类号: A61K9/5161 , A61K9/0019 , A61K9/19 , A61K9/4808 , A61K9/4891 , A61K47/6935 , A61K47/6939
摘要: The invention discloses a composition of chitosan-shelled nanoparticles and methods of manufacturing. The chitosan-shelled nanoparticles are characterized with a positive surface charge and enhanced epithelial ermeability for oral drug delivery.
摘要翻译: 本发明公开了一种壳聚糖壳形纳米颗粒的组成及其制造方法。 壳聚糖壳的纳米颗粒的特征在于具有正的表面电荷和增强的口服药物递送的上皮可渗透性。
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公开(公告)号:US07993624B2
公开(公告)日:2011-08-09
申请号:US12931202
申请日:2011-01-26
申请人: Hsing-Wen Sung , Hosheng Tu
发明人: Hsing-Wen Sung , Hosheng Tu
CPC分类号: A61K9/5146 , A61K9/0043 , A61K9/5161 , A61K31/13 , A61K31/445 , A61K31/55
摘要: The invention discloses nanoparticles composed of chitosan, poly-glutamic acid, and at least one bioactive agent characterized with a positive surface charge. The bioactive agent is hydrophobic or lipophilic in nature and is associated with micelles before being encapsulated in nanoparticles.
摘要翻译: 本发明公开了由壳聚糖,聚谷氨酸和至少一种以正表面电荷为特征的生物活性剂组成的纳米颗粒。 生物活性剂本质上是疏水性或亲脂性的,并且在包封在纳米颗粒中之前与胶束相关。
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公开(公告)号:US07988949B2
公开(公告)日:2011-08-02
申请号:US12931251
申请日:2011-01-27
申请人: Hsing-Wen Sung , Hosheng Tu
发明人: Hsing-Wen Sung , Hosheng Tu
CPC分类号: A61K9/4891 , A61K9/19 , A61K9/5146 , A61K9/5161 , A61K31/13 , A61K31/445 , A61K31/55 , A61K47/61
摘要: The invention discloses the nanoparticles composed of chitosan, poly-glutamic acid, and at least one antibiotics or equivalent bioactive agent characterized with a positive surface charge and their enhanced permeability in oral drug delivery.
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公开(公告)号:US07919072B1
公开(公告)日:2011-04-05
申请号:US12462576
申请日:2009-08-05
申请人: Hsing-Wen Sung , Hosheng Tu
发明人: Hsing-Wen Sung , Hosheng Tu
CPC分类号: A61K31/13 , A61K9/0043 , A61K9/19 , A61K9/2077 , A61K9/2081 , A61K9/2806 , A61K9/4891 , A61K9/5146 , A61K9/5161 , A61K9/5192 , A61K31/445 , A61K31/55 , A61K47/26 , A61K47/34 , A61K47/36
摘要: The invention discloses the bioactive nanoparticles composed of chitosan, poly-glutamic acid, and at least one bioactive agent characterized with a positive surface charge and enhanced permeability in oral drug delivery.
摘要翻译: 本发明公开了由壳聚糖,聚谷氨酸和至少一种生物活性剂组成的生物活性纳米颗粒,其特征在于具有阳性表面电荷并且在口服药物递送中具有增强的渗透性。
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公开(公告)号:US07897585B1
公开(公告)日:2011-03-01
申请号:US12378976
申请日:2009-02-20
申请人: Hsing-Wen Sung , Hosheng Tu
发明人: Hsing-Wen Sung , Hosheng Tu
IPC分类号: A01N43/04
CPC分类号: A61K31/13 , A61K9/0043 , A61K9/19 , A61K9/4891 , A61K9/5146 , A61K9/5153 , A61K9/5161 , A61K9/5192 , A61K31/445 , A61K31/55 , A61K38/37 , A61K38/4846 , A61K47/26
摘要: The invention discloses the nanoparticles composed of chitosan, poly-glutamic acid, and at least one anti-hemophilic factor or bioactive agent characterized with a positive surface charge and their enhanced permeability in oral drug delivery.
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