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公开(公告)号:US4370418A
公开(公告)日:1983-01-25
申请号:US286372
申请日:1981-07-24
CPC分类号: C12M41/44 , C12M29/10 , C12M41/48 , G05D9/04 , Y10T137/731 , Y10T137/86348
摘要: Liquid (13, 13a) within a vessel (12, 12a) which receives an inflow is maintained at a predetermined level (43) by discharging liquid as necessary to compensate for the inflow. Discharge flow is drawn from below the surface (18) of the liquid to avoid clogging from scum or from other undesirable effects of surface draw off. Intake structure (34) for the discharge pump (33, 33a) has a branched flow path (47) that includes a first inlet (48) situated above the predetermined level, a second inlet (49) situated below the predetermined level and a flow junction (51) through which both inlets are communicated with the discharge pump, the junction being at the predetermined liquid level. If the liquid rises above the flow junction, the pump draws liquid through the second, subsurface inlet. If the liquid surface recedes below the flow junction, the pump aspirates air or other gas through the first inlet. Consequently liquid level is stabilized at the elevation of the flow junction. The system is applicable, for example, to chemostats (11, 11a) in which microorganisms are cultured in a liquid medium.
摘要翻译: 接收流入的容器(12,12a)内的液体(13,13a)通过根据需要排出液体而保持在预定水平(43)以补偿流入。 从液体的表面(18)的下方抽出排出流,以避免从浮渣或其他不希望的表面抽出的影响堵塞。 用于排放泵(33,33a)的进气结构(34)具有分支流路(47),其包括位于预定水平高度的第一入口(48),位于预定水平以下的第二入口(49) 接头(51),两个入口与排放泵连通,该接合处于预定液位。 如果液体在流动接头上方升起,泵将液体通过第二个次表面的入口吸入。 如果液面低于流动接头,泵将吸入空气或其他气体通过第一个入口。 因此,液位在流动接头的高度处稳定。 该系统适用于例如在液体培养基中培养微生物的恒化器(11,11a)。
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公开(公告)号:US5871955A
公开(公告)日:1999-02-16
申请号:US662735
申请日:1991-02-28
申请人: Curtis Lajoie , Peter F. Strom
发明人: Curtis Lajoie , Peter F. Strom
CPC分类号: C12R1/38 , C07K14/21 , C07K14/325 , C12P1/04
摘要: Recombinant microorganisms which produce non-adaptive proteins in a mixed, microbiologically competitive environment and which utilize a selective substrate are disclosed as field application vectors.
摘要翻译: 公开了在混合,微生物竞争环境中产生非适应性蛋白质并利用选择性底物的重组微生物作为野外施用载体。
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