摘要:
A cloning and/or sequencing vector (1) comprises a nucleotide promoter sequence (3) and a nucleotide sequence (4) coding for a fusion protein active as a poison, which nucleotide sequence (4) is obtained by fusion of a coding nucleotide sequence (5) containing several unique cloning sites with a nucleotide sequence (6) coding for a poison protein, wherein the nucleotide sequences (3) and (4) are incorporated in an autonomous replication vector (2).
摘要:
The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.
摘要:
The present invention is related to specific sequences, preferably present in a diagnostic kit to identify if a female mammal comprises in her genome a mutation in the alpha-fetoprotein sequence or a partial or total deletion of this alpha-fetoprotein sequence, present heterozygously or homozygously (on both allele).
摘要:
The present invention is related to integrated method and tools to construct recombinant DNA molecules (to be used as DNA vaccine or gene therapy) without requiring the use of antibiotic(s) resistance gene(s) and without requiring the addition of one or more antibiotic(s) to the culture medium of cells submitted to this recombinant DNA method. The present invention allows to obtain the selection of recombinant host cell(s) transformed by a (exogenous) nucleic acid sequence of interest (extra-chromosomal vector containing the insert) and simultaneously stabilization (stable inheritance) of this (exogenous) nucleic acid sequence of interest into the transformed host cell(s) descendants (maintenance of the nucleic acid sequence of interest in the host cells population).
摘要:
The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.
摘要:
The present invention is related to a non-human genetically modified mammal comprising a mutation, a partial or total deletion of the genetic sequence encoding the wild type mammal alpha-fetoprotein.
摘要:
A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.
摘要:
A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.
摘要:
The present invention is related to a method for a high through-put screening detection of genetic modifications in genome engineering and a system for homologous recombination of an exogenous nucleotide sequence into a target cell genome sequence.
摘要:
A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.