Cloning and/or sequencing vector
    1.
    发明授权
    Cloning and/or sequencing vector 失效
    克隆和/或测序载体

    公开(公告)号:US5910438A

    公开(公告)日:1999-06-08

    申请号:US379614

    申请日:1995-07-20

    CPC分类号: C12N15/70

    摘要: A cloning and/or sequencing vector (1) comprises a nucleotide promoter sequence (3) and a nucleotide sequence (4) coding for a fusion protein active as a poison, which nucleotide sequence (4) is obtained by fusion of a coding nucleotide sequence (5) containing several unique cloning sites with a nucleotide sequence (6) coding for a poison protein, wherein the nucleotide sequences (3) and (4) are incorporated in an autonomous replication vector (2).

    摘要翻译: PCT No.PCT / BE93 / 00051 Sec。 371日期1995年7月20日 102(e)日期1995年7月20日PCT 1993年8月2日PCT公布。 第WO94 / 03616号公报 日期1994年2月17日克隆和/或测序载体(1)包含核苷酸启动子序列(3)和编码作为毒物活化的融合蛋白的核苷酸序列(4),该核苷酸序列(4)通过融合获得 的编码核苷酸序列(5),其含有编码毒蛋白的核苷酸序列(6)的几个独特的克隆位点,其中所述核苷酸序列(3)和(4)并入自主复制载体(2)中。

    Reversible, parallel and multitask cloning method and kit
    4.
    发明授权
    Reversible, parallel and multitask cloning method and kit 有权
    可逆,并行和多任务克隆方法和试剂盒

    公开(公告)号:US09309518B2

    公开(公告)日:2016-04-12

    申请号:US13660907

    申请日:2012-10-25

    摘要: The present invention is related to integrated method and tools to construct recombinant DNA molecules (to be used as DNA vaccine or gene therapy) without requiring the use of antibiotic(s) resistance gene(s) and without requiring the addition of one or more antibiotic(s) to the culture medium of cells submitted to this recombinant DNA method. The present invention allows to obtain the selection of recombinant host cell(s) transformed by a (exogenous) nucleic acid sequence of interest (extra-chromosomal vector containing the insert) and simultaneously stabilization (stable inheritance) of this (exogenous) nucleic acid sequence of interest into the transformed host cell(s) descendants (maintenance of the nucleic acid sequence of interest in the host cells population).

    摘要翻译: 本发明涉及构建重组DNA分子(用作DNA疫苗或基因治疗)而不需要使用抗生素抗性基因并且不需要添加一种或多种抗生素的综合方法和工具 到细胞的培养基中进行该重组DNA方法。 本发明允许获得通过(外源)感兴趣的核酸序列(含有插入片段的染色体外载体)转化的重组宿主细胞的选择,并同时稳定(稳定的遗传)该(外源)核酸序列 对转化的宿主细胞后代感兴趣(维持宿主细胞群体中感兴趣的核酸序列)。

    CLONING AND/OR SEQUENCING VECTOR
    7.
    发明申请
    CLONING AND/OR SEQUENCING VECTOR 审中-公开
    克隆和/或序列向量

    公开(公告)号:US20080299661A1

    公开(公告)日:2008-12-04

    申请号:US11842131

    申请日:2007-08-20

    IPC分类号: C12N15/74

    CPC分类号: C12N15/70

    摘要: A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.

    摘要翻译: 克隆和/或测序载体能够直接选择重组克隆。 载体编码融合蛋白,其包含蛋白质毒素。