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公开(公告)号：US08318497B2
公开(公告)日：2012-11-27
申请号：US10526525
申请日：2003-09-03
摘要： The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.
摘要翻译： 本发明涉及可逆的，平行的和/或多任务的克隆方法和试剂盒，其改进在核酸构建体（例如载体或细胞的染色体）中克隆（优选多个）遗传元件，并且快速 以及所述构建体在体外或体内以正确整合所述遗传元件的有效选择。
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公开(公告)号：US09309518B2
公开(公告)日：2016-04-12
申请号：US13660907
申请日：2012-10-25
摘要： The present invention is related to integrated method and tools to construct recombinant DNA molecules (to be used as DNA vaccine or gene therapy) without requiring the use of antibiotic(s) resistance gene(s) and without requiring the addition of one or more antibiotic(s) to the culture medium of cells submitted to this recombinant DNA method. The present invention allows to obtain the selection of recombinant host cell(s) transformed by a (exogenous) nucleic acid sequence of interest (extra-chromosomal vector containing the insert) and simultaneously stabilization (stable inheritance) of this (exogenous) nucleic acid sequence of interest into the transformed host cell(s) descendants (maintenance of the nucleic acid sequence of interest in the host cells population).
摘要翻译： 本发明涉及构建重组DNA分子（用作DNA疫苗或基因治疗）而不需要使用抗生素抗性基因并且不需要添加一种或多种抗生素的综合方法和工具 到细胞的培养基中进行该重组DNA方法。 本发明允许获得通过（外源）感兴趣的核酸序列（含有插入片段的染色体外载体）转化的重组宿主细胞的选择，并同时稳定（稳定的遗传）该（外源）核酸序列 对转化的宿主细胞后代感兴趣（维持宿主细胞群体中感兴趣的核酸序列）。
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公开(公告)号：US20130115658A1
公开(公告)日：2013-05-09
申请号：US13660907
申请日：2012-10-25
摘要： The present invention is related to integrated method and tools to construct recombinant DNA molecules (to be used as DNA vaccine or gene therapy) without requiring the use of antibiotic(s) resistance gene(s) and without requiring the addition of one or more antibiotic(s) to the culture medium of cells submitted to this recombinant DNA method. The present invention allows to obtain the selection of recombinant host cell(s) transformed by a (exogenous) nucleic acid sequence of interest (extra-chromosomal vector containing the insert) and simultaneously stabilization (stable inheritance) of this (exogenous) nucleic acid sequence of interest into the transformed host cell(s) descendants (maintenance of the nucleic acid sequence of interest in the host cells population).
摘要翻译： 本发明涉及构建重组DNA分子（用作DNA疫苗或基因治疗）而不需要使用抗生素抗性基因并且不需要添加一种或多种抗生素的综合方法和工具 到细胞的培养基中进行该重组DNA方法。 本发明允许获得通过（外源）感兴趣的核酸序列（含有插入片段的染色体外载体）转化的重组宿主细胞的选择，并同时稳定（稳定的遗传）该（外源）核酸序列 对转化的宿主细胞后代感兴趣（维持宿主细胞群体中感兴趣的核酸序列）。
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公开(公告)号：US20130011898A1
公开(公告)日：2013-01-10
申请号：US13178402
申请日：2011-07-07
申请人： Cédric Szpirer
发明人： Cédric Szpirer
CPC分类号： C12N15/70 , C12N7/00 , C12N2795/10351 , C12N2795/10352 , C12P1/04
摘要： The present invention relates to a genetically modified phage and use thereof in a method for producing a biomolecule of interest.
摘要翻译： 本发明涉及遗传修饰的噬菌体及其在生产感兴趣的生物分子的方法中的用途。
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