公开(公告)号：US4997915A

公开(公告)日：1991-03-05

申请号：US333964

申请日：1989-04-06

申请人： Larry U. L. Tan ,  Dirk Alkema ,  Gail Jackson ,  Po S. Wah

发明人： Larry U. L. Tan ,  Dirk Alkema ,  Gail Jackson ,  Po S. Wah

IPC分类号： C12P21/00 ,  A61K38/36 ,  A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07K1/16 ,  C07K1/22 ,  C07K14/005 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C07K16/00 ,  C12R1/01

CPC分类号： C07K14/235 ,  A61K39/00

摘要： Lymphocytosis promoting factor (LPF) and filamentous hemaglutinin (FHA) are isolated from the growth medium of the Bordatella pertussis organism and purified by selecting adsorbing the LPF and FHA on a selective adsorbing medium, such as filter aids or gel filtration media, at low ionic strength and subsequently removing the adsorbed LPF and FHA at using an aqueous medium of high ionic strength, either simultaneously or sequentially. Prior to desorbtion of the LPF and FHA, the adsorbing medium may be contacted with an aqueous solution of a non-ionic detergent, which enables the LPF and FHA subsequently desorbed to be substantially free from contamination by lipopolysaccharides (LPS). The LPF and FHA may be further purified on hydroxyapatite. The LPF and FHA may be detoxified separately or together by contacting with a cross-linking agent, such as glutaraldehyde or formaldehyde, in the presence of an anti-aggregation agent. The resulting purified and detoxified LPF and FHA may be used to formulate a vaccine against pertussis.