利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.012 秒)

    Preparation of a multicombinatorial library of antibody gene expression vectors
    4.
    发明授权
    Preparation of a multicombinatorial library of antibody gene expression vectors 有权
    制备抗体基因表达载体的多单体文库

    公开(公告)号：US06174708B1

    公开(公告)日：2001-01-16

    申请号：US09101629

    申请日：1999-01-22

    申请人： Regis Sodoyer ,  Luc Aujame ,  Fr{acute over (e)}d{acute over (e)}rique Geoffroy ,  Annabelle Bouchardon

    发明人： Regis Sodoyer ,  Luc Aujame ,  Fr{acute over (e)}d{acute over (e)}rique Geoffroy ,  Annabelle Bouchardon

    IPC分类号： C12P1934

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C07K16/1063 ,  C07K2319/00 ,  C12N15/1037

    摘要： On the basis of a first repertoire of genes coding for a population of one of two kinds of polypeptides capable of being optionally covalently combined, particularly variable regions of either the antibody light chain type or the antibody heavy chain type, and at least one gene coding for the other type of polypeptide, particularly a variable region of the other type, an antibody chain or preferably a second repertoire of genes coding for a population of said other type, the genes from the first repertoire are inserted into a first vector to form a population of vectors carrying the various genes of said first repertoire, and said gene of said other type or the genes from said second repertoire is/are inserted into a second vector. Both starting vectors have means enabling each to exchange one part by one or more irreversible recombinations to generate recombinant final vectors of which one contains a gene of one of said types and a gene of the other type.

    摘要翻译： 基于编码能够任选共价组合的两种多肽之一的群体的基因的第一个基因，特别是抗体轻链型或抗体重链型的可变区，以及至少一个编码 对于另一种类型的多肽，特别是另一种类型的可变区，抗体链或优选编码所述其他类型群体的基因的第二组氨酸，将来自第一个家族的基因插入到第一载体中以形成 携带所述第一组合物的各种基因的载体的载体，以及所述其他类型的所述基因或来自所述第二个目录的基因的插入到第二载体中。 两个起始载体都具有使每个能够通过一个或多个不可逆重组交换一部分以产生其中包含所述类型之一的基因和另一种类型的基因的重组终末载体的方法。

    Self-Replicating Vector Lacking An Antibiotic-Resistance Gene
    5.
    发明申请
    Self-Replicating Vector Lacking An Antibiotic-Resistance Gene 审中-公开
    自身复制载体缺乏抗生素抗性基因

    公开(公告)号：US20110207173A1

    公开(公告)日：2011-08-25

    申请号：US13054427

    申请日：2009-07-09

    申请人： Regis Sodoyer ,  Denis Speck ,  Isabelle Peubez ,  Nicolas Chaudet

    发明人： Regis Sodoyer ,  Denis Speck ,  Isabelle Peubez ,  Nicolas Chaudet

    IPC分类号： C12P21/00 ,  C12N15/63 ,  C12N1/00 ,  C12N1/21 ,  C12N15/74

    CPC分类号： C12N15/70 ,  A61K2039/53 ,  C12N15/74

    摘要： The subject matter of the present invention is a novel vector and the use thereof for producing a heterologous protein or a gene of interest, that can be used, for example, in the context of an immunization or gene therapy programme and concerns in particular a self-replicating vector lacking an antibiotic-resistance gene, comprising a sequence encoding the ccdA protein functionally linked to a first promoter, the sequence of the Cer locus and a heterologous sequence, functionally linked to a second promoter.

    摘要翻译： 本发明的主题是新型载体及其用于产生异源蛋白质或感兴趣的基因的用途，其可以用于例如免疫或基因治疗程序的上下文中，特别涉及自身 - 缺少抗生素抗性基因的复制载体，其包含编码与第一启动子功能性连接的ccdA蛋白的序列，Cer位点的序列和与第二启动子功能性连接的异源序列。

    Process for preparing a multicombinatorial library of vectors for expressing antibody genes
    6.
    发明授权
    Process for preparing a multicombinatorial library of vectors for expressing antibody genes 失效
    制备用于表达抗体基因的载体多组合文库的方法

    公开(公告)号：US06599697B1

    公开(公告)日：2003-07-29

    申请号：US08693234

    申请日：1996-10-04

    申请人： Regis Sodoyer ,  Luc Aujame ,  Frederique Geoffroy

    发明人： Regis Sodoyer ,  Luc Aujame ,  Frederique Geoffroy

    IPC分类号： C12O170

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C07K16/10 ,  C07K16/1063 ,  C07K2319/00 ,  C12N15/1037

    摘要： On the basis of a first repertoire of genes coding for a population of one of two kinds of polypeptides capable of being combined, particularly an antibody light chain variable region, and at least one gene, and preferably a second repertoire coding for the other kind of polypeptide, particularly an antibody heavy chain variable region, the genes from the first repertoire are inserted into a first vector to form a vector population and the genes from the second repertoire are inserted into a second vector, at least one of said vectors being a recipient for the expression of both genes as polypeptides irreversibly combined with the outer surface of the product of said vector.

    摘要翻译： 基于编码能够组合的两种能够组合的多肽，特别是抗体轻链可变区之一的群体的基因的第一个基因，以及至少一个基因，优选地编码另一种 多肽，特别是抗体重链可变区，将来自第一集合的基因插入第一载体中以形成载体群体，并将来自第二文库的基因插入第二载体中，所述载体中的至少一个为受体 用于将两个基因作为与所述载体的产物的外表面不可逆地组合的多肽的表达。