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公开(公告)号：US20100172899A1
公开(公告)日：2010-07-08
申请号：US12727162
申请日：2010-03-18
IPC分类号： A61K39/395 , C07K16/00 , C07K17/10 , C07K14/47
CPC分类号： C07K16/3084 , C07K16/00 , C07K2317/21 , C07K2317/52 , C07K2317/734 , G01N33/6854 , G01N33/686
摘要： IgM can be obtained in the form of a pentamer by placing the genes encoding the H, L, and J chains on the same vector to transform appropriate host cells. The gene encoding the J chain may be introduced by co-transfection. When no J chain is expressed, the IgM is produced as a hexamer. The transformants obtained according to the present invention achieve a high yield of IgM. The present invention also provides methods which enable separation and quantification of polymeric IgM.
摘要翻译： 通过将编码H，L和J链的基因置于相同载体上以转化合适的宿主细胞，可以以五聚体的形式获得IgM。 编码J链的基因可以通过共转染来引入。 当没有J链表达时，IgM作为六聚体产生。 根据本发明获得的转化体获得高产量的IgM。 本发明还提供能够分离和定量聚合物IgM的方法。
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公开(公告)号：US07709615B2
公开(公告)日：2010-05-04
申请号：US10564665
申请日：2004-07-15
IPC分类号： C07H21/04
CPC分类号： C07K16/3084 , C07K16/00 , C07K2317/21 , C07K2317/52 , C07K2317/734 , G01N33/6854 , G01N33/686
摘要： IgM can be obtained in the form of a pentamer by placing the genes encoding the H, L, and J chains on the same vector to transform appropriate host cells. The gene encoding the J chain may be introduced by co-transfection. When no J chain is expressed, the IgM is produced as a hexamer. The transformants obtained according to the present invention achieve a high yield of IgM. The present invention also provides methods which enable separation and quantification of polymeric IgM.
摘要翻译： 通过将编码H，L和J链的基因置于相同载体上以转化合适的宿主细胞，可以以五聚体的形式获得IgM。 编码J链的基因可以通过共转染来引入。 当没有J链表达时，IgM作为六聚体产生。 根据本发明获得的转化体获得高产量的IgM。 本发明还提供能够分离和定量聚合物IgM的方法。
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公开(公告)号：US08257703B2
公开(公告)日：2012-09-04
申请号：US12727162
申请日：2010-03-18
IPC分类号： A61K39/395
CPC分类号： C07K16/3084 , C07K16/00 , C07K2317/21 , C07K2317/52 , C07K2317/734 , G01N33/6854 , G01N33/686
摘要： IgM can be obtained in the form of a pentamer by placing the genes encoding the H, L, and J chains on the same vector to transform appropriate host cells. The gene encoding the J chain may be introduced by co-transfection. When no J chain is expressed, the IgM is produced as a hexamer. The transformants obtained according to the present invention achieve a high yield of IgM. The present invention also provides methods which enable separation and quantification of polymeric IgM.
摘要翻译： 通过将编码H，L和J链的基因置于相同载体上以转化合适的宿主细胞，可以以五聚体的形式获得IgM。 编码J链的基因可以通过共转染来引入。 当没有J链表达时，IgM作为六聚体产生。 根据本发明获得的转化体获得高产量的IgM。 本发明还提供能够分离和定量聚合物IgM的方法。
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4.发明申请IGM production by transformed cells and methods of quantifying said IgM production 有权通过转化细胞的IGM产生和量化所述IgM产生的方法公开(公告)号：US20070154469A1
公开(公告)日：2007-07-05
申请号：US10564665
申请日：2004-07-15
申请人： Reiko Irie
发明人： Reiko Irie
IPC分类号： A61K39/395 , G01N33/53 , C12N5/06 , C07K16/18
CPC分类号： C07K16/3084 , C07K16/00 , C07K2317/21 , C07K2317/52 , C07K2317/734 , G01N33/6854 , G01N33/686
摘要： IgM can be obtained in the form of a pentamer by placing the genes encoding the H, L, and J chains on the same vector to transform appropriate host cells. The gene encoding the J chain may be introduced by co-transfection. When no J chain is expressed, the IgM is produced as a hexamer. The transformants obtained according to the present invention achieve a high yield of IgM. The present invention also provides methods which enable separation and quantification of polymeric IgM.
摘要翻译： 通过将编码H，L和J链的基因置于相同载体上以转化合适的宿主细胞，可以以五聚体的形式获得IgM。 编码J链的基因可以通过共转染来引入。 当没有J链表达时，IgM作为六聚体产生。 根据本发明获得的转化体获得高产量的IgM。 本发明还提供能够分离和定量聚合物IgM的方法。
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公开(公告)号：US06589402B2
公开(公告)日：2003-07-08
申请号：US09765549
申请日：2001-01-19
申请人： Reiko Irie , Masayuki Suda , Toshihiko Sakuhara , Tatsuaki Ataka
发明人： Reiko Irie , Masayuki Suda , Toshihiko Sakuhara , Tatsuaki Ataka
IPC分类号： C25D1700
CPC分类号： B23H1/00 , B23H9/00 , C25D1/00 , C25D1/10 , Y10S204/12
摘要： A part fabricating apparatus has a holder for holding an object to be machined immersed in an electrolytic solution. A machining electrode subjects a surface of the object to an electrochemical reaction to electrolytically machine the surface of the object. The machining electrode has a sharp-edged tip and is coated with an insulator except for the sharp-edged tip. A spacing changing unit detects and changes a spacing between the surface of the object and the machining electrode. A potential/current control unit controls a potential/current on the machining electrode. An electrolytic solution changing unit supplies a first electrolytic solution for subjecting the surface of the object to be machined to a removal process to fabricate a cast mold, supplies a second electrolytic solution for depositing a first metal on a surface of the cast mold to form a first metal layer, and supplies a third electrolytic solution for depositing a second metal inside the cast mold to form a part.
摘要翻译： 零件制造装置具有用于将待加工物体保持浸入电解液中的保持器。 加工电极使物体的表面进行电化学反应，以电解加工物体的表面。 加工电极具有锋利边缘的尖端，并且除了锋利的尖端之外还涂覆有绝缘体。 间隔改变单元检测并改变物体的表面与加工电极之间的间隔。 电位/电流控制单元控制加工电极上的电位/电流。 电解液更换单元提供用于对被加工物的表面进行处理的第一电解液进行去除工艺以制造铸模，在铸模的表面上提供用于沉积第一金属的第二电解液，以形成 第一金属层，并且在铸模内部提供用于沉积第二金属的第三电解溶液以形成部分。
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公开(公告)号：US06221228B1
公开(公告)日：2001-04-24
申请号：US09204636
申请日：1998-12-02
申请人： Reiko Irie , Masayuki Suda , Toshihiko Sakuhara , Tatsuaki Ataka
发明人： Reiko Irie , Masayuki Suda , Toshihiko Sakuhara , Tatsuaki Ataka
IPC分类号： C25D110
CPC分类号： B23H1/00 , B23H9/00 , C25D1/00 , C25D1/10 , Y10S204/12
摘要： A part fabricating method comprises the steps of machining an object to fabricate a part cast mold, depositing a first metal on a surface of the cast mold to form a first metal layer, and depositing a second metal different in kind from the first metal inside the cast mold to form a part. The first metal layer is then selectively removed to take out the part formed inside the cast mold.
摘要翻译： 一种部件制造方法包括以下步骤：加工物体以制造部件铸模，在铸模的表面上沉积第一金属以形成第一金属层，以及将与第一金属不同种类的第二金属从第一金属 铸造模具组成部分。 然后选择性地去除第一金属层以取出在铸模内部形成的部分。
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