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公开(公告)号：US5656747A
公开(公告)日：1997-08-12
申请号：US145207
申请日：1993-11-03
CPC分类号： C07H13/06
摘要： The invention relates to a process for quantitatively purifying glycolipids, wherein the purification of the glycolipids is effected by acidifying the glycolipid-containing solution to pH .ltoreq.5.0, then heating the batch to 60.degree. C.-130.degree. C., and then cooling the batch down to .ltoreq.50.degree. C. and centrifuging it at .gtoreq.500 g in order to sediment the glycolipid-containing phase.
摘要翻译： 本发明涉及一种定量纯化糖脂的方法，其中糖脂的纯化是通过将含糖脂的溶液酸化至pH = 5.0，然后将批料加热至60℃-130℃，然后 将批料冷却至
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2.发明授权Pseudomonas aeruginosa and its use in a process for the biotechnological preparation of L-rhamnose 失效铜绿假单胞菌及其在L-鼠李糖生物技术制备过程中的应用
公开(公告)号：US5658793A
公开(公告)日：1997-08-19
申请号：US462027
申请日：1995-06-05
摘要： Bacterial strains are capable of the formation of rhamnolipids, which they generate in the culture solution. If bacteria of the type Pseudomonas aeruginosa are employed for the fermentation, these microorganisms synthesize rhamnolipids in a concentration of 70-120 g/l of culture solution. The L-rhamnose can be recovered directly from the culture solution by hydrolysis of the rhamnolipids, i.e. without a complicated separation of the cell material and without isolation of the rhamnolipids before hydrolysis.
摘要翻译： 细菌菌株能够形成它们在培养液中产生的鼠李糖脂。 如果将铜绿假单胞菌的细菌用于发酵，则这些微生物合成浓度为70-120g / l培养液的鼠李糖脂。 L-鼠李糖可以通过鼠李糖脂的水解从培养液中直接回收，即没有细胞材料的复杂分离，而在水解之前不分离鼠李糖脂。
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3.发明授权Process for reacting substrates with biocatalysts in a 2-phase liquid system 失效使底物与生物催化剂在2相液体体系中反应的方法
公开(公告)号：US4806477A
公开(公告)日：1989-02-21
申请号：US055541
申请日：1987-05-29
摘要： A process for reacting substrates with biocatalysts in a two-phase liquid system disposed in a columnar vessel having a top portion with an exit opening and a first filter disposed therein, a bottom portion with an entrance opening and a second filter disposed therein, a feed line connecting the exit and entrance openings, and a pump disposed in the feed line. The process comprises the steps of: stirring the two-phase liquid in the columnar vessel to accelerate sedimentation and minimize accumulation of the biocatalysts in the top portion of the vessel; selectively controlling the temperature of the two-phase liquid within the vessel; drawing, with the pump, a portion of the two-phase liquid through the exit opening and first filter; and passing the drawn-off portion of the two-phase liquid through the feed line and through the entrance opening and second filter into the vessel to enhance miscibility of the individual phases of the two-phase liquid with the vessel.
摘要翻译： 一种将底物与生物催化剂反应在两相液体系统中的方法，所述两相液体系统设置在具有顶部的柱状容器中，所述顶部具有出口和设置在其中的第一过滤器，底部具有入口和设置在其中的第二过滤器， 连接出口和入口的管线，以及设置在进料管线中的泵。 该方法包括以下步骤：搅拌柱状容器中的两相液体以加速沉淀并最小化生物催化剂在容器顶部的积聚; 选择性地控制容器内的两相液体的温度; 通过泵抽出一部分两相液体通过出口和第一过滤器; 并将两相液体的引出部分通过进料管线并通过入口和第二过滤器进入容器，以增强两相液体的各相与容器的混溶性。
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4.发明授权Pseudomonas aeruginosa and its use in a process for the biotechnological preparation of L-rhamnose 失效铜绿假单胞菌及其在L-鼠李糖生物技术制备过程中的应用
公开(公告)号：US5501966A
公开(公告)日：1996-03-26
申请号：US375230
申请日：1995-01-19
摘要： Pseudomonas aeruginosa and its use in a process for the biotechnological production of L-rhamnoseBacterial strains are capable of the formation of rhamnolipids, which they generate in the culture solution. If bacteria of the type Pseudomonas aeruginosa are employed for the fermentation, these microorganisms synthesize rhamnolipids in a concentration of 70-120 g/l of culture solution. The L-rhamnose can be recovered directly from the culture solution by hydrolysis of the rhamnolipids, i.e. without a complicated separation of the cell material and without isolation of the rhamnolipids before hydrolysis.
摘要翻译： 铜绿假单胞菌及其在L-鼠李糖生物技术生产过程中的应用细菌菌株能够在培养液中形成鼠李糖脂。 如果将铜绿假单胞菌的细菌用于发酵，则这些微生物合成浓度为70-120g / l培养液的鼠李糖脂。 L-鼠李糖可以通过鼠李糖脂的水解从培养液中直接回收，即没有细胞材料的复杂分离，而在水解之前不分离鼠李糖脂。
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