公开(公告)号：US20080271210A1

公开(公告)日：2008-10-30

申请号：US11664458

申请日：2005-10-01

申请人： Tatiana Kolesnik ,  Ritu Bhalla ,  Rengasamy Ramamoorthy ,  Srinivasan Ramachandran

发明人： Tatiana Kolesnik ,  Ritu Bhalla ,  Rengasamy Ramamoorthy ,  Srinivasan Ramachandran

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  C12N15/00 ,  C12N5/14

CPC分类号： C12N9/1205 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention is directed to a novel gene, Oslrk1, that is in involved in plant development, including root expansion. Methods of influencing this development are also described, as are transformed cells and transgenic plants comprising the described sequences.

摘要翻译： 本发明涉及涉及植物发育的新基因Oslrk1，其包括根扩展。 还描述了影响该发展的方法，以及包含所述序列的转化细胞和转基因植物也是如此。

公开(公告)号：US09273336B2

公开(公告)日：2016-03-01

申请号：US13164990

申请日：2011-06-21

申请人： Ritu Bhalla ,  Qi Chen ,  Qiong Cheng ,  Neeraj Pandey ,  Pierre E. Rouviere ,  Kristin Ruebling-Jass ,  Annapurna Sachan

发明人： Ritu Bhalla ,  Qi Chen ,  Qiong Cheng ,  Neeraj Pandey ,  Pierre E. Rouviere ,  Kristin Ruebling-Jass ,  Annapurna Sachan

IPC分类号： C12P21/06 ,  C12P21/02 ,  C12N1/02 ,  C12N9/10 ,  C12N15/70

CPC分类号： C12P21/02 ,  C12N1/02 ,  C12N9/1025 ,  C12N15/70

摘要： Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.

摘要翻译： 提供了获得具有增加重组微生物细胞的浮力密度或在重组微生物细胞内产生的包涵体的浮力密度的至少一种遗传修饰的重组微生物细胞的方法。 示例是增加表达异源肽和多肽的重组微生物细胞的浮力密度的遗传修饰。 在重组微生物细胞内增加基因ysaB，glyQ，glyS或其组合的表达产生具有较高浮力密度的细胞或包涵体。 通过减少或破坏内源性gltA基因的表达也可以获得类似的效果。 浮力密度的增加使肽生产在时间和成本方面更有效率。

公开(公告)号：US20120214202A1

公开(公告)日：2012-08-23

申请号：US13164990

申请日：2011-06-21

申请人： Ritu Bhalla ,  Qi Chen ,  Qiong Cheng ,  Neeraj Pandey ,  Pierre E. Rouviere ,  Kristin Ruebling-Jass ,  Annapurna Sachan

发明人： Ritu Bhalla ,  Qi Chen ,  Qiong Cheng ,  Neeraj Pandey ,  Pierre E. Rouviere ,  Kristin Ruebling-Jass ,  Annapurna Sachan

IPC分类号： C12P21/06 ,  C12N1/21

CPC分类号： C12P21/02 ,  C12N1/02 ,  C12N9/1025 ,  C12N15/70

摘要： Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.

摘要翻译： 提供了获得具有增加重组微生物细胞的浮力密度或在重组微生物细胞内产生的包涵体的浮力密度的至少一种遗传修饰的重组微生物细胞的方法。 示例是增加表达异源肽和多肽的重组微生物细胞的浮力密度的遗传修饰。 在重组微生物细胞内增加基因ysaB，glyQ，glyS或其组合的表达产生具有较高浮力密度的细胞或包涵体。 通过减少或破坏内源性gltA基因的表达也可以获得类似的效果。 浮力密度的增加使肽生产在时间和成本方面更有效率。