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1.发明申请Methods for Obtaining Optically Active Glycidyl Ethers and Optically Active Vicinal Diols from Racemic Substrates 审中-公开从外消旋底物获得光学活性缩水甘油醚和光活性唾液二醇的方法公开(公告)号：US20080213833A1
公开(公告)日：2008-09-04
申请号：US11872589
申请日：2007-10-15
CPC分类号： C12P7/18 , C12P17/02 , C12P41/002
摘要： The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific glycidyl ether hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active glycidyl ethers and associated optically active vicinal diols.
摘要翻译： 本发明提供酵母菌株和由这种酵母菌株的基因编码的具有对映体特异性缩水甘油醚水解酶活性的多肽。 本发明还具有编码这种多肽的核酸分子，含有该核酸分子的载体和含有该载体的细胞。 本发明还包括获得光学活性缩水甘油醚和相关的光学活性连位二醇的方法。
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2.发明申请Methods of Obtaining Optically Active Epoxides and Vicinal Diols from Styrene Oxides 审中-公开从苯乙烯氧化物获得光活性环氧化物和蓖麻醇的方法公开(公告)号：US20090275077A1
公开(公告)日：2009-11-05
申请号：US12372525
申请日：2009-02-17
CPC分类号： C12N9/14 , C12P7/22 , C12P17/02 , C12P41/00 , C12Y303/02009
摘要： The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific meso-epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining
摘要翻译： 本发明提供酵母菌株和由这种酵母菌株的基因编码的具有对映体特异性中环环氧化物水解酶活性的多肽。 本发明还具有编码这种多肽的核酸分子，含有该核酸分子的载体和含有该载体的细胞。 本发明还包括获得含有这种核酸分子的方法，以及含有这种载体的细胞。 本发明还包括用于获得
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3.发明申请Methods for Obtaining Optically Active Epoxides and Diols from 2,3-Disubstituted and 2,3-Trisubstituted Epoxides 审中-公开从2,3-二取代和2,3-三取代环氧化物获得光活性环氧化物和二醇的方法公开(公告)号：US20080199912A1
公开(公告)日：2008-08-21
申请号：US11872544
申请日：2007-10-15
申请人： Adriana Leonora Botes , Jeanette Lotter , Michel Labuschagne , Robin Kumar Mitra , Robyn Roth , Nasreen Abrahams , Clinton Simpson , Christiaan Van Der Westhuyzen
发明人： Adriana Leonora Botes , Jeanette Lotter , Michel Labuschagne , Robin Kumar Mitra , Robyn Roth , Nasreen Abrahams , Clinton Simpson , Christiaan Van Der Westhuyzen
CPC分类号： C12N9/14 , C12P7/18 , C12P17/02 , C12P41/001 , C12Y303/0201
摘要： The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific internal epoxide hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active internal epoxides and corresponding optically active internal diols.
摘要翻译： 本发明提供酵母菌株和由这种酵母菌株的基因编码的具有对映体内部环氧化物水解酶活性的多肽。 本发明还具有编码这种多肽的核酸分子，含有该核酸分子的载体和含有该载体的细胞。 本发明还包括获得光学活性内部环氧化物和相应的光学活性内部二醇的方法。
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公开(公告)号：US20080171359A1
公开(公告)日：2008-07-17
申请号：US11872496
申请日：2007-10-15
申请人： Adriana Leonora Botes , Michel Labuschagne , Robyn Roth , Robin Kumar Mitra , Jeanette Lotter , Rajesh Lalloo , Deepak Ramduth , Neeresh Rohitlall , Clinton Simpson , Petrus Van Zyl
发明人： Adriana Leonora Botes , Michel Labuschagne , Robyn Roth , Robin Kumar Mitra , Jeanette Lotter , Rajesh Lalloo , Deepak Ramduth , Neeresh Rohitlall , Clinton Simpson , Petrus Van Zyl
CPC分类号： C12N9/14 , C12N1/04 , C12N9/96 , C12N11/16 , C12N15/815
摘要： The invention provides isolated Y. lipolytica cells and substantially pure cultures of Y. lipolytica cells containing exogenous nucleic acids encoding EH polypeptides, e.g., enantioselective EH polypeptides. Also featured by the invention are methods for the production of the EH polypeptides and methods for hydrolysing epoxides and for producing optically active vicinal diols and/or optically active epoxides. Also embodied by the invention are efficient integrative expression vectors.
摘要翻译： 本发明提供了分离的解脂耶氏酵母细胞和含有编码EH多肽的外源核酸例如对映选择性EH多肽的解脂耶氏酵母细胞的基本上纯的培养物。 本发明还特征在于生产EH多肽的方法和用于水解环氧化物和产生光学活性连位二醇和/或光学活性环氧化物的方法。 本发明还体现了有效的综合表达载体。
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公开(公告)号：US07026144B2
公开(公告)日：2006-04-11
申请号：US10312996
申请日：2001-06-11
申请人： Jennifer Ann Chaplin , Neil Stockenstrom Gardiner , Robin Kumar Mitra , Christopher John Parkinson , Madrie Portwig , Butana Andrew Mboniswa , Melanie Daryl Evans-Dickson , Dean Brady , Stephanus Francois Marais , Shavani Reddy
发明人： Jennifer Ann Chaplin , Neil Stockenstrom Gardiner , Robin Kumar Mitra , Christopher John Parkinson , Madrie Portwig , Butana Andrew Mboniswa , Melanie Daryl Evans-Dickson , Dean Brady , Stephanus Francois Marais , Shavani Reddy
CPC分类号： C07C29/88 , C07B2200/07 , C07B2200/09 , C07C29/095 , C07C2601/14 , C07C35/12 , C07C33/20
摘要： A process of separating a single desired stereoisomer from a racemic mixture of eight stereoisomers of a compound of formula (III), wherein R1 represents an isopropanol group, an isopropyl group or an isopropylene group, includes the steps of: contacting the racemic mixture in a suitable organic solvent with an esterifying agent and a stereospecific enzyme which stereoselectively esterifies the —OH group of the desired stereoisomer, for a time sufficient to convert a desired percentage of the desired stereoisomer to a compound of formula (IV), wherein R1 is as defined above and R4 is an alkyl or an aryl group, to give a first reaction product including the compound of formula (IV), the organic solvent, the unconverted stereoisomers of the compound of formula (III), excess esterifying agent and by-products of the reaction; and separating the compound of formula (IV) from the first reaction product. The process is of particular application for the production of (−)-menthol
摘要翻译： 将单一所需立体异构体与式（III）化合物的八种立体异构体的外消旋混合物分离，其中R 1表示异丙醇基，异丙基或异丙烯基的方法包括步骤 使外消旋混合物在合适的有机溶剂中与酯化剂和立体选择性酯化所需立体异构体的-OH基团的立体选择性酯反应足够的时间以将所需百分比的所需立体异构体转化为式（III）化合物 IV），其中R 1如上所定义，R 4是烷基或芳基，得到包含式（IV）化合物的第一反应产物， 有机溶剂，式（III）化合物的未转化的立体异构体，过量的酯化剂和反应的副产物; 并将式（IV）化合物与第一反应产物分离。 该方法特别适用于生产（ - ） - 薄荷醇
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