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公开(公告)号：US08501451B2
公开(公告)日：2013-08-06
申请号：US13238227
申请日：2011-09-21
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶和制备和使用它们的方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US20090162906A1
公开(公告)日：2009-06-25
申请号：US11868909
申请日：2007-10-08
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶和制备和使用它们的方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US07026144B2
公开(公告)日：2006-04-11
申请号：US10312996
申请日：2001-06-11
申请人： Jennifer Ann Chaplin , Neil Stockenstrom Gardiner , Robin Kumar Mitra , Christopher John Parkinson , Madrie Portwig , Butana Andrew Mboniswa , Melanie Daryl Evans-Dickson , Dean Brady , Stephanus Francois Marais , Shavani Reddy
发明人： Jennifer Ann Chaplin , Neil Stockenstrom Gardiner , Robin Kumar Mitra , Christopher John Parkinson , Madrie Portwig , Butana Andrew Mboniswa , Melanie Daryl Evans-Dickson , Dean Brady , Stephanus Francois Marais , Shavani Reddy
CPC分类号： C07C29/88 , C07B2200/07 , C07B2200/09 , C07C29/095 , C07C2601/14 , C07C35/12 , C07C33/20
摘要： A process of separating a single desired stereoisomer from a racemic mixture of eight stereoisomers of a compound of formula (III), wherein R1 represents an isopropanol group, an isopropyl group or an isopropylene group, includes the steps of: contacting the racemic mixture in a suitable organic solvent with an esterifying agent and a stereospecific enzyme which stereoselectively esterifies the —OH group of the desired stereoisomer, for a time sufficient to convert a desired percentage of the desired stereoisomer to a compound of formula (IV), wherein R1 is as defined above and R4 is an alkyl or an aryl group, to give a first reaction product including the compound of formula (IV), the organic solvent, the unconverted stereoisomers of the compound of formula (III), excess esterifying agent and by-products of the reaction; and separating the compound of formula (IV) from the first reaction product. The process is of particular application for the production of (−)-menthol
摘要翻译： 将单一所需立体异构体与式（III）化合物的八种立体异构体的外消旋混合物分离，其中R 1表示异丙醇基，异丙基或异丙烯基的方法包括步骤 使外消旋混合物在合适的有机溶剂中与酯化剂和立体选择性酯化所需立体异构体的-OH基团的立体选择性酯反应足够的时间以将所需百分比的所需立体异构体转化为式（III）化合物 IV），其中R 1如上所定义，R 4是烷基或芳基，得到包含式（IV）化合物的第一反应产物， 有机溶剂，式（III）化合物的未转化的立体异构体，过量的酯化剂和反应的副产物; 并将式（IV）化合物与第一反应产物分离。 该方法特别适用于生产（ - ） - 薄荷醇
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公开(公告)号：US20110263839A1
公开(公告)日：2011-10-27
申请号：US13169853
申请日：2011-06-27
申请人： Grace DESANTIS , Jennifer Ann CHAPLIN , Ellen CHI , Aileen MILAN , Jay M. SHORT , David WEINER , Mark MADDEN , Darcy MADDEN , Mark J. BURK , Dan E. ROBERTSON
发明人： Grace DESANTIS , Jennifer Ann CHAPLIN , Ellen CHI , Aileen MILAN , Jay M. SHORT , David WEINER , Mark MADDEN , Darcy MADDEN , Mark J. BURK , Dan E. ROBERTSON
IPC分类号： C07H21/04
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US20120009633A1
公开(公告)日：2012-01-12
申请号：US13238227
申请日：2011-09-21
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶及其制备和使用方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US08034595B2
公开(公告)日：2011-10-11
申请号：US11868909
申请日：2007-10-08
申请人： Mark Madden , David Weiner , Jennifer Ann Chaplin
发明人： Mark Madden , David Weiner , Jennifer Ann Chaplin
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention provides nitrilases and methods for making and using them, and in one aspect, provides methods for producing enantiomerically pure α-substituted carboxylic acids, such as, for example, α-amino acids and α-hydroxy acids. In one aspect, methods of the invention combine an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity, to stereoselectively hydrolyze the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the carboxylic acid.
摘要翻译： 本发明提供腈水解酶及其制备和使用方法，一方面，提供了制备对映体纯的α-取代羧酸的方法，例如α-氨基酸和α-羟基酸。 在一个方面，本发明的方法在腈水解酶或具有腈水解酶活性的多肽的存在下，将醛或酮与氰化物和氨或铵盐或胺组合，在条件下立体选择性水解氨基腈或氰醇中间体 足以产生羧酸。
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公开(公告)号：US20090176976A1
公开(公告)日：2009-07-09
申请号：US12397204
申请日：2009-03-03
申请人： Grace DESANTIS , Jennifer Ann Chaplin , Ellen Chi , Aileen Milan , Jay M. Short , David Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson , Darcy Madden
发明人： Grace DESANTIS , Jennifer Ann Chaplin , Ellen Chi , Aileen Milan , Jay M. Short , David Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson , Darcy Madden
IPC分类号： C12N15/11
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US08334125B2
公开(公告)日：2012-12-18
申请号：US13169853
申请日：2011-06-27
申请人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Darcy Madden, legal representative , Mark Burk , Dan Robertson
发明人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Mark Burk , Dan Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , C12Q1/68 , A61K38/00 , C07H21/04 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US07993901B2
公开(公告)日：2011-08-09
申请号：US12629818
申请日：2009-12-02
申请人： Grace Desantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Marcy Madden, legal representative , Mark Burk , Dan Robertson
发明人： Grace Desantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay Short , David Weiner , Mark Madden , Mark Burk , Dan Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , C12Q1/68 , A61K38/00 , C07H21/04 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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公开(公告)号：US20100086986A1
公开(公告)日：2010-04-08
申请号：US12629818
申请日：2009-12-02
申请人： Grace DESANTIS , Jennifer Ann CHAPLIN , Ellen CHI , Aileen MILAN , Jay M. SHORT , David WEINER , Mark MADDEN , Darcy MADDEN , Mark J. BURK , Dan E. ROBERTSON
发明人： Grace DESANTIS , Jennifer Ann CHAPLIN , Ellen CHI , Aileen MILAN , Jay M. SHORT , David WEINER , Mark MADDEN , Darcy MADDEN , Mark J. BURK , Dan E. ROBERTSON
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。
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