公开(公告)号：US07632801B2

公开(公告)日：2009-12-15

申请号：US10337213

申请日：2003-01-06

Applicant: Peter M. Palese ,  Robert O'Neill ,  Ronald Harty

Inventor： Peter M. Palese ,  Robert O'Neill ,  Ronald Harty

IPC: A61K38/00 ,  A61K38/08 ,  A61K38/10 ,  C07K4/00 ,  C07K4/02 ,  C07K7/00

CPC classification number: C07K14/005 ,  A61K38/00 ,  C12N15/1055 ,  C12N2760/16122 ,  G01N33/56983 ,  G01N2333/11

Abstract: The present invention relates to the identification of host cell proteins that interact with viral proteins required for virus replication, and high throughput assays to identify compounds that interfere with the specific interaction between the viral and host cell protein. Interfering compounds that inhibit viral replication can be used therapeutically to treat viral infection. The invention is based, in part, on the Applicants' discovery of novel interactions between viral proteins and a human host cell proteins. One of these host cell proteins, referred to herein as NPI-1, interacts with influenza virus protein NP. Also, host cell proteins, referred to herein as NS1I-1 and NS1-BP interact with influenza virus protein NS1. In addition, host cell proteins containing WW domains that interact with viral proteins such as Rhabdoviral M protein are described. Compounds that interfere with the binding of the host cell and viral proteins, and inhibit viral replication can be useful for treating viral infection in vivo.

Abstract translation: 本发明涉及鉴定与病毒复制所需的病毒蛋白相互作用的宿主细胞蛋白质，以及用于鉴定干扰病毒和宿主细胞蛋白质之间特异性相互作用的化合物的高通量测定。 抑制病毒复制的干扰化合物可用于治疗性治疗病毒感染。 本发明部分地基于申请人发现病毒蛋白和人宿主细胞蛋白之间的新的相互作用。 这些宿主细胞蛋白之一，本文称为NPI-1，与流感病毒蛋白NP相互作用。 此外，本文称为NS1I-1和NS1-BP的宿主细胞蛋白与流感病毒蛋白NS1相互作用。 此外，描述了含有与病毒蛋白如Rhabdoviral M蛋白相互作用的WW结构域的宿主细胞蛋白质。 干扰宿主细胞和病毒蛋白结合并抑制病毒复制的化合物可用于在体内治疗病毒感染。

公开(公告)号：US06503703B1

公开(公告)日：2003-01-07

申请号：US09636791

申请日：2000-08-11

Applicant: Peter M Palese ,  Robert O'Neill ,  Ronald Harty

Inventor： Peter M Palese ,  Robert O'Neill ,  Ronald Harty

IPC: C12Q100

CPC classification number: C07K14/005 ,  A61K38/00 ,  C12N15/1055 ,  C12N2760/16122 ,  G01N33/56983 ,  G01N2333/11

Abstract: The present invention relates to the identification of host cell proteins that interact with viral proteins required for virus replication, and high throughput assays to identify compounds that interfere with the specific interaction between the viral and host cell protein. Interfering compounds that inhibit viral replication can be used therapeutically to treat viral infection. The invention is based, in part, on the Applicants' discovery of novel interactions between viral proteins and a human host cell proteins. One of these host cell proteins, referred to herein as NPI-1, interacts with influenza virus protein NP. Also, host cell proteins, referred to herein as NS1I-1 and NS1-BP interact with influenza virus protein NS1. In addition, host cell proteins containing WW domains that interact with viral proteins such as Rhabdoviral M protein are described. Compounds that interfere with the binding of the host cell and viral proteins, and inhibit viral replication can be useful for treating viral infection in vivo.