公开(公告)号：US4316954A

公开(公告)日：1982-02-23

申请号：US141748

申请日：1980-04-18

申请人： Roy E. Snoke ,  Theodore W. Esders

发明人： Roy E. Snoke ,  Theodore W. Esders

IPC分类号： C12Q1/26 ,  C12Q1/34 ,  C12Q1/527 ,  C12Q1/00

CPC分类号： C12Q1/26 ,  C12Q1/34 ,  C12Q1/527 ,  G01N2333/988 ,  Y10S435/805

摘要： A composition and element for assaying a neuraminic acid comprises a neuraminic acid aldolase, pyruvate oxidase and an electron acceptor. A sample containing a neuraminic acid can be contacted with the composition and the product detected spectrophotometrically.

摘要翻译： 用于测定神经氨酸的组合物和元件包括神经氨酸醛缩酶，丙酮酸氧化酶和电子受体。 可以将含有神经氨酸的样品与组合物接触，并用分光光度法检测产物。

公开(公告)号：US3983005A

公开(公告)日：1976-09-28

申请号：US565897

申请日：1975-04-07

申请人： Charles T. Goodhue ,  Hugh A. Risley ,  Roy E. Snoke ,  Gary M. Underwood

发明人： Charles T. Goodhue ,  Hugh A. Risley ,  Roy E. Snoke ,  Gary M. Underwood

IPC分类号： C12Q1/60 ,  C12K1/04

CPC分类号： C12Q1/60 ,  Y10S435/805 ,  Y10S435/839 ,  Y10S435/897 ,  Y10S435/918

摘要： An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, a support. Cholesterol oxidase and a composition for the hydrolysis of cholesterol esters comprising lipase having cholesterol esterase activity and protease are included in the element such that cholesterol esters contained in a sample applied to the spreading layer are saponified to free cholesterol and free cholesterol is decomposed in the presence of cholesterol oxidase to produce a detectable change related to the total cholesterol content of the sample.

摘要翻译： 公开了用于分析其胆固醇含量的液体的整体分析元件。 该元件是包括至少两个叠加层的类型，包括扩散层和流体接触的试剂层，以及任选的支撑体。 胆固醇氧化酶和用于水解包含具有胆固醇酯酶活性的脂肪酶的胆固醇酯和蛋白酶的组合物包括在元件中，使得包含在施加到铺展层上的样品中的胆固醇酯被皂化以游离胆固醇并且游离胆固醇在存在下分解 的胆固醇氧化酶以产生与样品的总胆固醇含量相关的可检测的变化。

公开(公告)号：US4751178A

公开(公告)日：1988-06-14

申请号：US780508

申请日：1985-09-26

申请人： Bruce E. Babb ,  Roy E. Snoke

发明人： Bruce E. Babb ,  Roy E. Snoke

IPC分类号： C07D215/12 ,  C07D217/14 ,  C07D217/18 ,  C09B23/14 ,  C12Q1/48 ,  G01N33/52

CPC分类号： C09B23/145 ,  C12Q1/48 ,  Y10S435/805

摘要： New substrates for .gamma.-glutamyltransferase (.gamma.-GT) are disclosed. These subtrates are useful in the colorimetric determination of .gamma.-GT since the cleavage of the .gamma.-glutamyl group produces a detectable species--an aminostyrylquinolinium dye. The substrates have the formula: ##STR1## wherein: R and R.sub.1 are independently selected from hydrogen, alkyl and alkoxy andQ is ##STR2## wherein: R.sub.2 is alkyl having from 1 to 4 carbon atoms;R.sub.3 and R.sub.4 are independently selected from hydrogen, alkyl, alkoxy, nitro, cyano and halogen; andX.sup..crclbar. is an anion.

摘要翻译： 公开了γ-谷氨酰转移酶（γ-GT）的新底物。 由于γ-谷氨酰基的裂解产生可检测的物质 - 氨基苯乙烯基喹啉染料，这些底物可用于γ-GT的比色测定。 底物具有下式：其中：R和R 1独立地选自氢，烷基和烷氧基，Q是其中：R 2是具有1至4个碳原子的烷基; R 3和R 4独立地选自氢，烷基，烷氧基，硝基，氰基和卤素; 而X（ - ）是阴离子。

公开(公告)号：US4374930A

公开(公告)日：1983-02-22

申请号：US312474

申请日：1981-10-19

申请人： Roy E. Snoke ,  Charles T. Goodhue

发明人： Roy E. Snoke ,  Charles T. Goodhue

IPC分类号： C12N9/04 ,  C07G7/028

CPC分类号： C12N9/0006 ,  Y10S435/815

摘要： A method for the purification and recovery of cholesterol oxidase is disclosed. Cholesterol oxidase is purified by a method including the steps of:(a) contacting a solution of the cholesterol oxidase to be purified with a chromatography composition comprising an unbound water-insoluble steroid which is a nonsubstrate for cholesterol oxidase so as to adsorb the cholesterol oxidase on the steroid;(b) separating the solution from the steroid; and(c) contacting the steroid with an eluting solution containing a surfactant at a concentration between 0.001 percent and 10 percent so as to recover the adsorbed cholesterol oxidase.In a preferred embodiment, the solution containing the cholesterol oxidase to be purified is a microorganism growth medium having cholesterol oxidase activity and, prior to the step of contacting the solution with a steroid, the solution is contacted with an ion-exchange chromatography material. In yet another embodiment, the eluting solution containing the surfactant also contains a metal salt of a concentration of at least about 0.005 molar. There is also disclosed a method for forming a packed bed of a composition comprising the unbound steroid which comprises the step of first suspending the steroid in a water-miscible organic solvent.

摘要翻译： 公开了一种纯化和回收胆固醇氧化酶的方法。 胆固醇氧化酶通过包括以下步骤的方法纯化：（a）将待纯化的胆固醇氧化酶的溶液与包含未结合的水不溶性类固醇（其是胆固醇氧化酶的非底物）的色谱组合物接触，以吸附胆固醇氧化酶 对类固醇; （b）将溶液与类固醇分离; 和（c）使类固醇与含有0.001％至10％浓度的表面活性剂的洗脱溶液接触，以回收吸附的胆固醇氧化酶。 在优选的实施方案中，含有待纯化的胆固醇氧化酶的溶液是具有胆固醇氧化酶活性的微生物生长培养基，并且在将溶液与类固醇接触的步骤之前，将溶液与离子交换色谱材料接触。 在另一个实施方案中，含有表面活性剂的洗脱溶液还含有至少约0.005摩尔浓度的金属盐。 还公开了一种形成包含未结合类固醇的组合物的填充床的方法，该方法包括首先将类固醇悬浮在水混溶性有机溶剂中的步骤。